The invention provides a method for rapidly cultivating
Bletilla striata seedlings under natural light, and relates to the technical field of
plant bioengineering. The method includes the following steps: (1) selecting mature capsules of
Bletilla striata as explants; (2) inoculating the capsules on a
germination medium after disinfection treatment, seeds turning to green after 7-10 days, and protocorms being formed after 40-50 days; (3) inoculating the protocorms on a subculture multiplication and differentiation medium to cultivate; and (4)
cutting cluster buds off multiplicated and differentiated seedlings, performing subculture multiplication, inoculating seedlings whose
plant heights are above 3 cm to
seedling hardening and rooting mediums, and
transplanting the seedlings when the seedlings grow to 5-7 cm. 1-5 g / L of self-made
artemisia argyi dry leaf
powder is added in subculture multiplication and rooting culture phases; the cultivation process of
test tube seedlings is to cultivate the seedlings under outdoor natural light; and therefore, the
stress resistance of
tissue culture seedlings can be strengthened, production costs can be reduced, a large amount of sterile proliferating seedlings can be obtained, and the seedlings can be put into production one year in advance under artificially controlled conditions; and compared with traditional breeding methods, the method has a
transplanting survival rate increased by about 30% and a production cost reduced by 20%, and is suitable for large scale production of seedlings.