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A silk fibroin/gelatin interpenetrating network hydrogel capable of cell encapsulation and its preparation method

A silk fibroin, interpenetrating network technology, applied in the field of tissue engineering materials, can solve the problems of uncontrollable biological factors, poor mechanical properties of hydrogels, etc. The effect of the preparation process

Active Publication Date: 2021-11-30
CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, physical factors have the disadvantages of poor mechanical properties of hydrogels, chemical factors have the introduction of toxic cross-linking reagents, and biological factors have many uncontrollable conditions.

Method used

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  • A silk fibroin/gelatin interpenetrating network hydrogel capable of cell encapsulation and its preparation method
  • A silk fibroin/gelatin interpenetrating network hydrogel capable of cell encapsulation and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1, a kind of preparation method of the silk fibroin / gelatin interpenetrating network hydrogel that can realize cell wrapping, comprises the steps:

[0035] 1) Mix the silk fibroin solution with a mass fraction of 8% and the modified gelatin solution with a mass fraction of 8% according to a mass ratio of 10:0 to form a prepolymer solution, and add photoinitiator 2-hydroxyl to the prepolymer solution -4'-(2-hydroxyethoxy)-2-methylpropiophenone, the mass volume ratio (g / ml) of photoinitiator and prepolymer solution is 0.1%;

[0036] 2) Ultrasonic treatment step 1) The ultrasonic time of the obtained solution is 35s, and the solution has fluidity and transparency after ultrasonication;

[0037] 3) According to the ratio of the number of cells added and the volume ratio of the solution obtained in step 2) to 1 million cells / mL, the solution obtained in step 2) was pipetted repeatedly with the cells to mix evenly to obtain a cell pre-aggregation solution, and 130uL of cells ...

Embodiment 2

[0039] 1, a kind of preparation method of the silk fibroin / gelatin interpenetrating network hydrogel that can realize cell wrapping, comprises the steps:

[0040] 1) Mix the silk fibroin solution with a mass fraction of 8% and the modified gelatin solution with a mass fraction of 8% according to a mass ratio of 9::1 to form a prepolymer solution, and add photoinitiator 2- Hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone, the mass volume ratio (g / ml) of photoinitiator and prepolymer solution is 0.1%;

[0041] 2) Ultrasonic treatment step 1) The ultrasonic time of the obtained solution is 40s, and the solution has fluidity and transparency after ultrasonication;

[0042] 3) According to the ratio of the number of cells added and the volume ratio of the solution obtained in step 2) to 1 million cells / mL, the solution obtained in step 2) was pipetted repeatedly with the cells to mix evenly to obtain a cell pre-aggregation solution, and 130uL of cells was taken / The pre-polymer...

Embodiment 3

[0044] 1, a kind of preparation method of the silk fibroin / gelatin interpenetrating network hydrogel that can realize cell wrapping, comprises the steps:

[0045] 1) Mix the silk fibroin solution with a mass fraction of 8% and the modified gelatin solution with a mass fraction of 8% according to a mass ratio of 8:2 to form a prepolymer solution, and add photoinitiator 2-hydroxyl to the prepolymer solution -4'-(2-hydroxyethoxy)-2-methylpropiophenone, the mass volume ratio (g / ml) of photoinitiator and prepolymer solution is 0.1%;

[0046] 2) Ultrasonic treatment step 1) The ultrasonic time of the obtained solution is 49s, and the solution has fluidity and transparency after ultrasonication;

[0047] 3) According to the ratio of the number of cells added and the volume ratio of the solution obtained in step 2) to 1 million cells / mL, the solution obtained in step 2) was pipetted repeatedly with the cells to mix evenly to obtain a cell pre-aggregation solution, and 130uL of cells w...

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Abstract

The invention discloses a method for preparing a silk fibroin / gelatin interpenetrating network hydrogel capable of encapsulating cells, comprising the following steps: 1) mixing a silk fibroin solution and a modified gelatin solution to form a prepolymer solution, and feeding Adding a photoinitiator to the prepolymer solution; 2) Ultrasonic treatment of the solution obtained in step 1), the solution has fluidity and transparency after ultrasonic treatment; 3) The solution obtained in step 2) is mixed evenly with cells to obtain a cell prepolymerization solution, and further Place the cell pre-polymerization solution in a petri dish; 4) put the petri dish obtained in step 3) under ultraviolet light for photo-crosslinking to form a gel, and then place it in a 37°C incubator until the mold solution turns milky white. . The method can realize cell adhesion proliferation and three-dimensional encapsulation in situ, and is beneficial to the three-dimensional construction of cartilage tissue in vitro. The maximum swelling rate of the prepared hydrogel is 11.3754±0.29; the maximum elastic modulus can reach 600kPa±5kpa; it has strong enzymatic degradation resistance.

Description

technical field [0001] The invention belongs to the field of tissue engineering materials, and in particular relates to a silk fibroin / gelatin interpenetrating network hydrogel which can realize cell wrapping and a preparation method thereof. Background technique [0002] Cartilage tissue is a special connective tissue that does not contain blood vessels, lymph and nerves. Once damaged, it is very easy to induce diseases such as arthritis, thereby making its function dysfunction. It is difficult to achieve self-repair after cartilage damage mainly because of the inability to provide blood vessels, resulting in the inability to spontaneously gather fibrous clots after the defect. And because of the strong differentiation ability of chondrocytes, it is difficult to move and grow in the missing part after injury. And this ability to divide will gradually weaken with age, and the replacement ability of cell matrix will also decrease. Therefore, the treatment of cartilage defect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071A61L27/22A61L27/38A61L27/52
CPCA61L27/222A61L27/227A61L27/3817A61L27/52A61L2430/06C08L89/00
Inventor 肖文谦李加乐谭云飞月相智任博跃
Owner CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY
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