Preparation process of lyophilized human thrombin
A technology of human thrombin and preparation process, applied in the field of preparation technology of human thrombin, can solve the problems of long activation time, unstable thrombin, protein denaturation, etc. simple effect
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[0069] Embodiment 1: Taking 250 liters of plasma as an example, the specific preparation process is as follows:
[0070] (1) Preparation of cryoprecipitated plasma
[0071] Raw material plasma collected according to the requirements of Part Three of the 2015 edition of the "Chinese Pharmacopoeia", which has passed the re-inspection and passed the quarantine period, is sterilized with 75% ethanol in the pre-melting room, and then rinsed with water for injection below 35 °C to exhaust the ethanol. , to break the bag. After the bag is broken, it is transported to the melting tank, and the circulating water of 30-35 °C is used for interlayer circulation to melt the slurry; after the plasma is melted, the hot water circulation is stopped in time, and the temperature of the plasma is controlled between 0-4 °C, and the sample is sent for quality inspection. Anti-HIV, HBsAg, anti-HCV, anti-HBs and microbial limit inspection; after filtration or centrifugation, the centrifugation speed ...
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