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Method for measuring contents of four active components in healthcare food Liuweigelan Jiangzhi tablets

An active ingredient, the technology of Lanjiangzhi Tablets, applied in the field of medicine, can solve problems such as difficulty in comprehensive and accurate control of drug quality, instability of other components, and few detection indicators, achieving good reproducibility and stability, simple method, Simple operation effect

Inactive Publication Date: 2018-10-16
GUANGXI UNIV OF CHINESE MEDICINE
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  • Claims
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Problems solved by technology

At present, domestic methods for the determination of one or two active ingredients in Liuwei Gelan Jiangzhi Tablets have been published. These methods have few detection indicators, which may lead to instability or lack of other components, making it difficult to fully and accurately control the quality of the drug

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  • Method for measuring contents of four active components in healthcare food Liuweigelan Jiangzhi tablets
  • Method for measuring contents of four active components in healthcare food Liuweigelan Jiangzhi tablets
  • Method for measuring contents of four active components in healthcare food Liuweigelan Jiangzhi tablets

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present invention, but the present invention is not limited thereto.

[0022] 1 Materials and Instruments

[0023] 1.1 Materials Puerarin reference substance was purchased from Shanghai Ronghe Pharmaceutical Technology Co., Ltd. (batch number 170109, purity > 98%); daidzein reference substance was purchased from Chengdu Mansite Biotechnology Co., Ltd. (Lot number MUST-17101202, purity is 99.99%); Orange cassiatin (Cheysophanol) reference substance is purchased from China Institute for Food and Drug Control, (Lot number is 111900-201303, purity is 99.1%); Chrysophanol (Aurantio-obtusin) The reference substance was purchased from Chengdu Mansite Biotechnology Co., Ltd. (batch number MUST-17041310, with a purity of 99.50%); 10 batches of Liuwei Gelan Jiangzhi Tablets were provided by the Pha...

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Abstract

The invention discloses a method for simultaneously measuring contents of four active components puerarin, daidzein, aurantio-obtusin and chrysophanol in Liuweigelan Jiangzhi tablets by an HPLC method. The method adopts the following chromatographic conditions: a chromatographic column is Phenomenex Gemini C18 column (250 mm*4.6 mm, 5 Mum); a flow rate is 1 ml.min-1; a detection wavelength is 286nm; a column temperature is 30 DEG C; and a sample amount is 15 MuL; under the chromatographic conditions, chromatographic peaks are well separated; concentrations and peak areas of the puerarin, daidzein, aurantio-obtusin and chrysophanol show a good linear relationship. The method is simple, rapid, accurate, good in reproducibility, and capable of providing a theoretical basis for comprehensiveevaluation and control of quality of the Liuweigelan Jiangzhi tablets.

Description

technical field [0001] The invention relates to a method for detecting the content of four active ingredients in Liuwei Gelan Jiangzhi Tablets, a health food, in particular to a method for simultaneously measuring the four active ingredients in Liuwei Gelan Jiangzhi Tablets, which belongs to the technical field of medicine. Background technique [0002] Hyperlipidemia is a common and frequently occurring metabolic and endocrine disease, which seriously endangers human health; Western medicines currently used for the treatment of hyperlipidemia (such as statins, fibrates, almoxes, niacin, biliary Acid chelating agents, etc.) are favored by everyone because of their quick onset and definite curative effect, but Western medicine has limitations such as single target, single lipid-lowering effect, and varying degrees of toxic and side effects. However, traditional Chinese medicine has the unique advantages of multi-pharmacological activities, multi-targets, and multi-link coordi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 蒋林农毅清周雅琪
Owner GUANGXI UNIV OF CHINESE MEDICINE
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