Screening of synergistic flora of carbon, nitrogen, and phosphorus for promoting root salt resistance and preparation and application of synergistic bacterial agents

Abstract

A screening of synergistic flora of carbon, nitrogen, and phosphorus that promotes root resistance to salt and the preparation and application of synergistic bacterial agents belong to the field of environmental biotechnology. In this method, the collected root samples of wild plants in saline-alkali land are firstly treated with an activity-maintaining buffer solution. The root microbial sample suspension is obtained by cleaning, and then the root microbial sample suspension is collected by centrifugal sedimentation and microporous membrane interception method to collect rhizosphere microbial samples and make solid microbial inoculation raw materials; then use specialized high-salt carbon-deficient liquid medium and Biologically active and inert carbon sources, to screen out the synergistic salt-resistant bacteria group of the heterogeneous carbon source utilization type in the solid-state microbial inoculation raw materials; further use the specialized phosphorus-dissolving and nitrogen-deficient liquid medium to screen out the synergistic salt-resistant bacteria group of the heterogeneous carbon source utilization type Salt-tolerant carbon, nitrogen, phosphorus synergistic flora in the bacterial flora; the salt-tolerant carbon, nitrogen, and phosphorus synergistic flora is mixed with a microbial activity maintenance carrier to prepare a solid preparation of the carbon, nitrogen, and phosphorus synergistic flora that can promote root salt resistance. Promotes salt tolerance of crop roots and improves their growth.

Description

Technical field [0001] The present invention belongs to the field of environmental biological technology, and more particularly to the screening of carbonaceous phosphorus and syndrome with a root resistant salt and preparation and application of synergists. Background technique [0002] At present, my country's salted land is as high as 180 million mu, and it has incremented year by year. Taking the facility vegetable soil as an example, the content of salting in the surface soil is 7.9 times the exposed ground, and the total amount of soil salt in the facilities of the facility has increased by 69.3%. Soil salting has seriously hindered the growth of crops and the sustainable development of planting industry. Therefore, how to improve crop salt tolerance is one of the important issues facing the benign development of my country's growing development. [0003] Freshwater resources shortages are an important factor in restricting my country's agricultural development. In particul...

AI Technical Summary

Problems solved by technology

[0006] The existing microbial agents and their preparation methods for improving the salt tolerance of crop roots are mostly focused on the screening of salt-tolerant strains, but the problems of nitrogen, phosphorus, and carbon uptake by plant roots are often ignored.

Images