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Application of serum lipid biomarkers in the early diagnosis of nsclc

A biomarker, serum lipid technology, applied in the application field of serum lipid biomarkers in the early diagnosis of NSCLC, can solve the problems of limited research scope and inability to analyze heat unstable metabolites

Active Publication Date: 2021-06-15
HUZHOU CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gas chromatography / mass spectrometry (GC-MS) has high sensitivity, but its research scope is limited to the analysis of volatile substances, and cannot analyze metabolites with thermal instability and large molecular weight

Method used

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  • Application of serum lipid biomarkers in the early diagnosis of nsclc
  • Application of serum lipid biomarkers in the early diagnosis of nsclc
  • Application of serum lipid biomarkers in the early diagnosis of nsclc

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Embodiment Construction

[0044] The present invention will be further described below in conjunction with specific examples, but the present invention is not limited to these specific implementations. Those skilled in the art will realize that the present invention covers all alternatives, modifications and equivalents as may be included within the scope of the claims.

[0045] see figure 1 The application method of serum lipid biomarkers of the present invention in the early diagnosis of NSCLC includes two parts, specifically as follows:

[0046] 1. Non-target NSCLC lipidomics detection and analysis based on UHPLC-Q-TOF / MS technology

[0047] (1) Instruments and reagents

[0048] ① Instrument

[0049] Ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry, including Agilent 1290 ultra-high performance liquid chromatography and ABSciex 6600 triple time-of-flight mass spectrometer (AB Sciex, USA), Phenomenex Kinetex C18 100A chromatographic column (1.7μm, 2.1×100mm ...

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Abstract

The application method of serum lipid biomarkers in the early diagnosis of NSCLC, including: collecting serum samples of NSCLC patients, patients with benign lung lesions and normal people; Time mass spectrometry method was used to detect lipid metabolism markers in serum samples; NSCLC-related differential lipid metabolism markers were screened by multivariate pattern recognition analysis; KEGG analysis and metabolic pathway analysis of NSCLC differential lipid metabolites were used, Screen out the key metabolic pathways with the highest correlation with lipid metabolites; conduct "gene-enzyme-reaction-metabolite" network analysis of NSCLC differential lipid metabolites to obtain NSCLC differential lipid metabolite network diagram; synthesize NSCLC differential lipid metabolites Based on the results of lipid metabolism marker screening and metabolic pathway analysis, serum lipid biomarkers for early diagnosis of NSCLC were screened.

Description

technical field [0001] The invention relates to an application method of serum lipid biomarkers in the early diagnosis of NSCLC. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world, and it has become the first cause of death from malignant tumors in urban population in my country. Non-small cell lung cancer (NSCLC) includes squamous cell carcinoma (squamous cell carcinoma), adenocarcinoma, and large cell carcinoma. Compared with small cell carcinoma, the growth and division of its cancer cells are slower, and the spread and metastasis are relatively late. Non-small cell lung cancer accounts for about 80% of all lung cancers, and about 75% of the patients are in the advanced stage when they are discovered, and the 5-year survival rate is very low. Traditional imaging and sputum exfoliation cytology are still the main means of screening for early lung cancer, but there are certain missed and misdiagnosed cases. Although pathologic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N30/02G01N30/72
CPCG01N30/02G01N30/72G01N33/574G01N33/57423G01N33/57484G01N2570/00G01N2800/12
Inventor 陈莹蓉戴利成马志红沈雄荣
Owner HUZHOU CENT HOSPITAL
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