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Method for extracting and separating cyclic adenosine monophosphate from jujube

A technology for cyclic adenosine monophosphate and jujube, which is applied in the field of extraction and separation of cyclic adenosine monophosphate, can solve problems such as difficulty in separation, and achieves the effects of simple process operation and low price

Inactive Publication Date: 2018-10-23
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is an urgent need to provide a fast, convenient, and low-cost pectin-removing treatment method to solve the difficult problem of cAMP separation.

Method used

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  • Method for extracting and separating cyclic adenosine monophosphate from jujube
  • Method for extracting and separating cyclic adenosine monophosphate from jujube

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A method for extracting and isolating cyclic adenosine monophosphate from jujube, the method comprises the steps of:

[0023] (1) Remove the core of the non-mildew jujube, freeze it with liquid nitrogen, and then crush it, add ultrapure water according to the ratio of material to liquid 1:25-35, adjust the aqueous solution to pH 2.5-3.5 with hydrochloric acid, and heat it in a water bath at 90-100°C for 45 -55min, centrifuge to take the supernatant, repeat the extraction once, and combine the two supernatants to obtain cyclic adenosine monophosphate extract A.

[0024] (2) Adjust the pH of the supernatant to 10.0-12.0 with sodium hydroxide, and let it stand at room temperature for 50-60 minutes to obtain the extract B.

[0025] (3) Use hydrochloric acid to adjust the pH of the supernatant after standing to 4.0-4.5, according to V (supernatant): V (CaCl 2 solution)=10:1 by adding CaCl 2 The solution was allowed to stand at room temperature for 30-40 minutes, and after ...

Embodiment 2

[0030] According to a kind of method for extracting and separating cyclic adenosine monophosphate from jujube described in embodiment 1, the method comprises the steps:

[0031] A method for extracting and isolating cyclic adenosine monophosphate from jujube, the method comprises the steps of:

[0032] (1) Remove the core of the non-mildew jujube and freeze it with liquid nitrogen, then crush it, add ultrapure water according to the ratio of material to liquid 1:25, adjust the pH of the aqueous solution to 2.5 with hydrochloric acid, heat it in a water bath at 90°C for 45 minutes, and centrifuge to take the supernatant , repeat the extraction once, and combine the two supernatants to obtain cyclic adenosine monophosphate extract A.

[0033] (2) Adjust the pH of the supernatant to 10.0 with sodium hydroxide, and let it stand at room temperature for 50 minutes to obtain the extract B.

[0034] (3) Use hydrochloric acid to adjust the pH of the supernatant after standing to 4.0, ...

Embodiment 3

[0039] According to embodiment 1 or 2, a kind of method for extracting and isolating cyclic adenosine monophosphate from jujube, the method comprises the steps:

[0040] A method for extracting and isolating cyclic adenosine monophosphate from jujube, the method comprises the steps of:

[0041] (1) De-pitted non-mildew jujube, frozen with liquid nitrogen, crushed, added ultrapure water according to the ratio of material to liquid 1:35, adjusted the pH of the aqueous solution to 3.5 with hydrochloric acid, heated in a water bath at 90-100°C for 55 minutes, and centrifuged to take the The supernatant was repeatedly extracted once, and the two supernatants were combined to obtain cyclic adenosine monophosphate extract A.

[0042] (2) Adjust the pH of the supernatant to 12.0 with sodium hydroxide, and let it stand at room temperature for 60 minutes to obtain the extract B.

[0043] (3) Use hydrochloric acid to adjust the pH of the supernatant after standing to 4.5, and add CaCl a...

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Abstract

The invention discloses a method for extracting and separating cyclic adenosine monophosphate from jujube. The method comprises the following steps: grinding jujube with an ultrafine grinder, adjusting the pH of an extraction solvent to 3.0 with hydrochloric acid, adding ultrapure water and performing water-bath extraction, centrifuging and taking a supernatant so as to obtain a crude extract of cyclic adenosine monophosphate; regulating the pH of the crude extract to 12.0 with NaOH, standing at room temperature for 60 min, regulating the pH of the crude extract to 4.5 with hydrochloric acid,adding a CaCl2 solution, standing at room temperature for 30 min, carrying out centrifugal separation, and collecting a supernatant; using H103 type macroporous adsorption resin to carry out column chromatography testing, wherein the process parameters are as follows: pH of a sample solution is 5, concentration of the sample solution is 30 mcirograms / mL, flow rate of the sample solution is 2 mL / min; and eluting with a 30% ethanol solution at the elution rate of 2 mL / min. Materials and reagents required for the method for extracting and separating cyclic adenosine monophosphate from jujube arelow in cost, and the technological operation is simple. The method is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of methods for extracting and isolating cyclic adenosine monophosphate from jujube, in particular to a method for extracting and isolating cyclic adenosine monophosphate from jujube. Background technique [0002] Cyclic Adenosine monophosphate (cAMP) is the abbreviation of "adenosine-3', 5'-cyclized monophosphate", also known as "cyclic adenosine monophosphate" and "cyclized adenosine monophosphate". cAMP is a cyclic nucleotide, which is formed by condensation of adenosine triphosphate (ATP) after being catalyzed by adenylyl cyclase (AC). Soluble in water, stable to acid and heat, it is an important small molecular substance that participates in the regulation of intracellular substance metabolism and biological functions, and is the "second messenger" for the transmission of life information. cAMP can regulate the biosynthesis of sugar, fat and protein in cells, participate in the metabolic process of vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/213C07H1/08
CPCC07H1/08C07H19/213
Inventor 李景明倪元颖马丽艳崔慧温海超刘璇君
Owner CHINA AGRI UNIV
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