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Method for detecting doping of antibiotic filter residues in rapeseed meal

A technology of antibiotics and rapeseed meal, which is applied in the field of detecting whether rapeseed meal is mixed with antibiotic filter residue, can solve the problems of endangering the health of farmed animals and affecting food safety, and achieve the effects of short detection time, accurate discrimination and non-destructive testing

Active Publication Date: 2018-10-23
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the filter residue contains incompletely extracted antibiotic residues and secondary metabolites that have not been evaluated for safety, the use of feed materials containing antibiotic filter residues in the breeding process will endanger the health of farmed animals, affect food safety, and induce bacterial resistance, etc. potential risks

Method used

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  • Method for detecting doping of antibiotic filter residues in rapeseed meal
  • Method for detecting doping of antibiotic filter residues in rapeseed meal
  • Method for detecting doping of antibiotic filter residues in rapeseed meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Sample pretreatment

[0039] Rapeseed meal, streptomycin filter residue, and rapeseed meal mixed with streptomycin filter residue (10%, m / m) were respectively pulverized by cyclone mill, and the particle size after pulverization reached 18 mesh. Weigh 0.5 g of crushed samples into clean glass headspace bottles respectively, seal with rubber stoppers, and seal with aluminum caps to ensure that the headspace bottles are airtight.

[0040] (2) Static headspace volatile component collection

[0041] Place the headspace vials filled with samples on a heat incubator for heating and incubation to promote the release of volatile substances in the sample into the headspace. The heat incubation temperature is 90°C, the incubation time is 10min, and the incubator vibration speed is 500rpm. After the thermal incubation, the volatile components in the static headspace were aspirated with a sampling needle, with a volume of 500 μL and an injection speed of 100 μL / s.

[0042] (3...

Embodiment 2

[0061] (1) Sample pretreatment

[0062] The rapeseed meal, the cephalosporin filter residue, and the rapeseed meal mixed with the cephalosporin filter residue (10%, m / m) were respectively crushed by a cyclone mill, and the particle size after crushing reached 18 mesh. Weigh 0.5g of crushed samples into clean glass headspace vials, seal with rubber stoppers, and seal with aluminum caps to ensure that the headspace vials are airtight.

[0063] (2) Static headspace volatile component collection

[0064] Place the headspace bottle filled with the sample on the heat incubator for heating and incubation to promote the release of volatile substances in the sample to the headspace. The heat incubation temperature is 90°C, the incubation time is 10min, and the vibration speed of the incubator is 500rpm. After the thermal incubation, the volatile components in the static headspace were aspirated with a sampling needle, with a volume of 500 μL and an injection speed of 100 μL / s.

[006...

Embodiment 3

[0082] (1) Sample pretreatment

[0083] Rapeseed meal, colistin sulfate filter residue, and rapeseed meal mixed with colistin sulfate filter residue (10%, m / m) were respectively pulverized by a cyclone mill, and the particle size after pulverization reached 18 mesh. Weigh 0.5 g of the pulverized samples into clean glass headspace bottles, seal with rubber stoppers, and seal with aluminum caps to ensure that the headspace bottles are airtight.

[0084] (2) Static headspace volatile component collection

[0085] Place the headspace bottle filled with the sample on the heat incubator for heating and incubation to promote the release of volatile substances in the sample to the headspace. The heat incubation temperature is 90°C, the incubation time is 10min, and the vibration speed of the incubator is 500rpm. After the thermal incubation, the volatile components in the static headspace were aspirated with a sampling needle, with a volume of 500 μL and an injection speed of 100 μL / ...

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Abstract

The invention discloses a method for detecting whether antibiotic filter residues are doped in rapeseed meal, comprising the following steps: detecting whether rapeseed meal contains characteristic markers of antibiotic filter residues, and detecting the characteristic markers. That is to say, antibiotic filter residues are doped in the rapeseed meal. The method of the invention specifically contains the following steps: pretreatment of samples, collection of volatile components, gas chromatography separation, and ion mobility spectrum detection for detecting whether there are characteristic markers of antibiotic filter residues in a sample so as to determine whether antibiotic filter residues are doped in rapeseed meal. The method of the invention has high separating power of gas chromatography and high sensitivity of ion mobility spectrum. Meanwhile, by a static headspace sampling mode, accurate and nondestructive testing of trace volatile components can be achieved. The method can be adopted to detect characteristic markers of streptomycin filter residue, cephalosporins filter residue and colistin sulfate filter residue to identify adulteration of rapeseed meal.

Description

technical field [0001] The invention relates to a method for detecting whether rapeseed meal is mixed with antibiotic filter residue, and belongs to the field of feed quality and safety detection. Background technique [0002] Rapeseed meal is a by-product of oil extraction from rapeseed. Its protein content is between 34% and 38%, and the proportion of methionine and lysine in the amino acid composition is relatively high. [0003] Antibiotic filter residue is a kind of high-concentration industrial waste containing a variety of refractory organic substances formed during the production of antibiotics by biological fermentation. High protein content. Since the filter residue contains incompletely extracted antibiotic residues and secondary metabolites that have not been evaluated for safety, the use of feed materials containing antibiotic filter residues in the breeding process will endanger the health of farmed animals, affect food safety, and induce bacterial resistance,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N30/60G01N27/64
CPCG01N1/28G01N27/64G01N30/60G01N2030/025
Inventor 李阳樊霞肖志明贾铮索德成刘晓露王石
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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