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Tetracycline antibiotics degrading arthrobacterium and application thereof

A tetracycline and antibiotic technology, applied in bacteria, microorganisms, microorganisms, etc., can solve the problem of limited antibiotic-degrading strains

Active Publication Date: 2018-10-26
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Overall, the antibiotic-degrading strains obtained through screening are relatively limited, especially the strains that can degrade multiple antibiotics at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] 1. Arthrobacter Arthrobacter sp. The method for domestication, screening and identification comprises the following steps:

[0029] 1) Take about 5g of sludge and add them to nutrient-poor enrichment medium and nutrient-enrichment medium containing target antibiotics (1, oxytetracycline, 50mg / L) respectively, and put them in a constant temperature shaker (30°C, 150°C -180 rpm) for one week in the dark.

[0030] 2) After the culture of the previous stage was allowed to stand, 5 mL of the supernatant was taken and transferred to the same resistance-enriched medium. After culturing for 7 days, the residual concentration of antibiotics was determined. A total of 3 cycles of enrichment were performed.

[0031] 3) Compare the antibiotic residue concentration in the oligotrophic enrichment culture and the eutrophic enrichment culture, select the enrichment culture with a higher antibiotic degradation rate, and after standing for 5-10min, take 0.2mL of the culture and spread ...

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PUM

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Abstract

The invention relates to the field of environment protection, and discloses a tetracycline antibiotics degrading arthrobacterium and application thereof. The classification name of the tetracycline antibiotics degrading arthrobacterium is Arthrobacter sp.. The Arthrobacter sp. is tetracycline antibiotics degrading arthrobacterium, can effectively degrade various tetracycline antibiotics, has the best oxytetracycline degrading effect, and has the good degrading effect for tetracycline and aureomycin. The bacterial strain OTC-16 can grow under the pH of 6.0-9.0 and under the temperature of 20-35DEG C, the application range is wide, and the tetracycline antibiotics degrading arthrobacterium can be used for repairing tetracycline antibiotics pollution.

Description

technical field [0001] The invention relates to the field of environmental protection, in particular to a tetracycline antibiotic degrading Arthrobacter and its application. Background technique [0002] Antibiotic residues are common in the environment. Antibiotics have been detected in soil, sewage sludge, surface water, and animal and plant foods, causing harm to the human body and the ecological environment. Many studies have shown that the detectable concentration of antibiotics in environmental soil is close to the content level of other pesticide organic pollutants in soil. How to effectively remove antibiotic residues in the environment has become an important environmental problem to be solved urgently. [0003] Biodegradation is an effective means to solve the environmental residues of antibiotics, which has attracted extensive attention of scholars at home and abroad. Currently, tetracycline-degrading bacteria have been reported. Tetracycline antibiotic-degradi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C02F3/34B09C1/10C12R1/06
CPCB09C1/10C02F3/34C12N1/20C12N1/36C12N1/205C12R2001/06
Inventor 林辉张昕马军伟孙万春叶静俞巧钢王强邹平陈照明符建荣
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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