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Gene related to late bolting characters of radishes and application of gene

A radish and trait technology, applied in the field of genetic engineering, can solve the problem of not finding the gene sequence, and achieve the effect of saving the cost and time of field management of plants

Active Publication Date: 2018-10-26
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are obvious sequence variations at the RsFLC gene locus, but no new gene sequences related to the late bolting trait of radish were found

Method used

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  • Gene related to late bolting characters of radishes and application of gene
  • Gene related to late bolting characters of radishes and application of gene
  • Gene related to late bolting characters of radishes and application of gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Obtaining of Gene Sequences Related to Bolting Resistance Traits in Radish

[0037] The present invention uses conventional map-based cloning methods to identify and clone genes related to bolting resistance traits of radish.

[0038] 1. Genetic segregation population construction and phenotype identification

[0039] Taking the late bolting material "Ninengo" from Japan and the Chinese early bolting material "Maer" as the parents, the F with 183 individual plants was constructed. 2 group. In the spring of 2016 to parents, F 1 , F 2 The bolting and flowering traits of individual plants in the population were investigated. The method of investigation is to investigate the budding and flowering conditions of individual plants every other day. The budding time is the number of days from planting until the bud is visible to the naked eye; the flowering time is the number of days from planting to the opening of the first flower. The budding time and flowering time of the late bo...

Embodiment 2

[0061] Application of the 1627bp insert sequence in RsFLC2 in the identification of late bolting and early bolting materials of radish

[0062] 1. Identification of the 1627bp insert sequence in RsFLC2 in late bolting and early bolting radish

[0063] 1) DNA extraction

[0064] The conventional CTAB method was used to extract the genomic DNA of 183 kinds of radish materials to be tested in Table 2. The 183 kinds of radish materials to be tested in Table 2 were F1 generations obtained by crossing parents Ninengo and Maer, and F2 individual plants obtained by F1 selfing.

[0065] 2) PCR amplification and detection

[0066] Use 1F (5'-ATGGGAAGAAAAAAACTAGAGAT-3') and 1R (5'-TGCATTAATCCGTGGTAAATT-3') primers for PCR amplification of the radish material to be tested.

[0067] PCR reaction system: contains 100ng genomic DNA, 2μl 10×PCR Buffer, 1.6μl dNTPs, 0.4μl each (10μM) of the upstream and downstream primers, 1.5UTaq enzyme, plus ddH 2 O to 30μl. The PCR amplification procedure is: 94°C pr...

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Abstract

The invention provides a gene related to late bolting characters of radishes and application of the gene, and belongs to the technical field of gene engineering. The gene is provided with nucleotide sequences shown as SEQ ID No.1. The gene and the application have the advantage that the radishes can bolt and bloom late by the aid of the gene.

Description

Technical field [0001] The present invention relates to the technical field of genetic engineering, in particular to a gene related to late bolting traits of radish and its application. Background technique [0002] Radish originated in my country and is an important vegetable crop in my country. It has long been the "home-dish" of urban and rural residents in my country. The annual planting area is 16 million mu, accounting for 6% of my country's entire vegetable production. The radish is a long-day plant. After low-temperature vernalization, it turns from vegetative growth to reproductive growth under long-day conditions, thereby bolting and flowering. The most important effect of immature bolting and flowering on radish production is the transformation from vegetative growth to reproductive growth, which inhibits the expansion of fleshy roots, resulting in a decline in yield and quality. The discovery of late bolting genes in turnips and the cultivation of late bolting varie...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张丽王庆彪
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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