Method for identifying Japanese ginseng rhizomes and non-generic confusion or counterfeit species of Japanese ginseng rhizomes based on ITS2

Abstract

The invention relates to a method for identifying Japanese ginseng rhizomes and non-generic confusion or counterfeit species of the Japanese ginseng rhizomes based on ITS2, and discloses a method foridentifying the Japanese ginseng rhizomes and the non-generic confusion or counterfeit species of the Japanese ginseng rhizomes according to DNA (deoxyribonucleic acid) level. The method includes thesteps: 1) extracting Japanese ginseng rhizome DNA of a sample to be measured; 2) taking the DNA of the sample to be measured as a template and performing PCR (polymerase chain reaction) amplificationon fragments of an ITS2 sequence; 3) positively sequencing a PCR product and performing ITS2 database shearing to obtain the ITS2 sequences of the Japanese ginseng rhizomes; 4) building an NJ phylogenetic tree, analyzing intraspecific and interspecific average genetic distances and comparing secondary structural features. Results indicate that the ITS2 sequences of the Japanese ginseng rhizomes are clustered together, the intraspecific average genetic distances of the Japanese ginseng rhizomes are remarkably shorter than the interspecific average genetic distances of the non-generic confusionor counterfeit species of the Japanese ginseng rhizomes, secondary structures of the Japanese ginseng rhizomes widely differ from those of the non-generic confusion or counterfeit species, and the ITS2 sequences are used for rapidly and accurately identifying the Japanese ginseng rhizomes and the non-generic confusion or counterfeit species of the Japanese ginseng rhizomes. The method is simple tooperate, high in resolution ratio and strong in applicability and repeatability, and the Japanese ginseng rhizomes and the non-generic confusion or counterfeit species of the Japanese ginseng rhizomes can be rapidly and accurately identified.

Description

technical field [0001] The invention relates to a method for quickly identifying non-congener imitation products in which bamboo joints are involved based on the ITS2 sequence. Background technique [0002] Bamboo ginseng is a plant of the Araliaceae genus Panax ginseng ( Panax japonicus The dry rhizome of C. A. Mey.), also known as Beisanqi, Zhujiesanqi, etc., belongs to the rare and endangered Chinese herbal medicine in my country, and is called "the king of herbs". Bamboo ginseng is warm in nature, sweet in taste, slightly bitter, and returns to the liver, spleen, and lung meridian. It also has the effects of northern medicine ginseng tonic and strength, and southern medicine Sanqi invigorating blood circulation and removing blood stasis. It is mainly used for post-illness weakness and injury from falls . Modern pharmacological studies have shown that saponins, volatile oils, sugars, amino acids and other chemical substances are the medicinal components of bamboo ginse...

AI Technical Summary

Problems solved by technology

[0006] Purpose of the present invention: In order to overcome the limitations and complex technical deficiencies of methods such as the traditional traits of Panax ginseng, tissue microscopic identification, etc., provide an ITS2 fragment of ribosomal DNA to identify Panax ginseng and its non-congenus mixed counterfeit products Methods

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