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Tissue culture and rejuvenizing method for adult male populus tomentosa

A technology of Populus tomentosa and optimization methods, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve problems such as failure to pay enough attention to reproductive materials, unfavorable growth genetic transformation, and difficulty in ensuring juvenile sex, etc. To achieve the effect of facilitating the expansion of reproduction, avoiding the effect of maturation, and obvious effect of juvenile

Inactive Publication Date: 2018-11-02
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the tissue culture production process of Populus tomentosa, insufficient attention has not been paid to the juvenile nature of the propagating material. In order to achieve the purpose of rapid emergence and early profit, tissue culture mostly directly picks branches from the mother tree or uses water After accelerating the germination of the cultivated branches, take the stem tip or the stem section with axillary buds as explants. It is difficult to guarantee the juvenile nature of such explants, and it is easy to age during the subculture process, and it is not conducive to future growth and genetic transformation operations. caused huge economic losses to forestry production

Method used

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  • Tissue culture and rejuvenizing method for adult male populus tomentosa
  • Tissue culture and rejuvenizing method for adult male populus tomentosa
  • Tissue culture and rejuvenizing method for adult male populus tomentosa

Examples

Experimental program
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Effect test

Embodiment 1

[0029]Embodiment 1 A kind of adult male Populus tomentosa tissue culture juvenile method, comprises the following steps:

[0030] 1) Collect the root shoots of 30-year-old male Populus tomentosa grown in the current year. The root shoots need to be moderately lignified (semi-lignified). Cut the strips into 3cm and rinse them with tap water. Move the rinsed root strips to the operating table, soak and sterilize them with 10% sodium hypochlorite for 10 minutes, and then cut the sterilized root strips into 2cm sections. The stem segment has 1 bud to obtain the explant;

[0031] 2) Inoculate the explants into MS medium for basic culture. The culture conditions are as follows: the culture temperature is 23°C, the light time is 12h / d, and the light intensity for culture is 2000lx; sterile vaccine;

[0032] 3) Cut the sterile seedlings into 1.0cm-sized budded stem segments, inoculate them into MS medium containing 0.7mg / L6-BA and 0.01mg / LNAA for 3 generations of subculture, and tra...

Embodiment 2

[0035] Embodiment 2 A kind of adult male Populus tomentosa tissue culture juvenile method, comprises the following steps:

[0036] 1) Collect the 200-year-old male Populus tomentosa in Qianxinfang Village, Daxing District, Beijing as the object, and take the root shoots that were born in that year as propagules. The root shoots need to be moderately lignified (semi-lignified), and the root shoots are removed. If the buds are not full at the top and bottom, take the root shoots from the middle section and cut them into 4cm, rinse them with tap water, move the rinsed root shoots to the operating table, soak and sterilize them with 0.1% mercuric chloride solution for 7 minutes, and then put the sterilized The root shoots of the root sprouts are cut into 2cm segments, and the root shoots stem section has 2 buds to obtain explants;

[0037] 2) Inoculate the explants into MS medium for basal culture. The culture conditions are as follows: the culture temperature is 25°C, the light t...

Embodiment 3

[0041] Example 3 Subculture cycle and growth of 200-year-old male Populus tomentosa tissue-cultured clones

[0042] In embodiment 2, after the buds are transferred to the proliferation medium, the clones that are moved into the proliferation medium are recorded as 1 generation, and the growth situation of each generation clones is continuously observed, and the graftable buds that are greater than 1.0cm in the subculture stage are regarded as effective buds. Calculate the proliferation coefficient of the buds, record the number of bottle seedlings with a height of ≥3cm and ≤3cm, and observe the stem height growth of the multiplication culture clone bottle seedlings. When the growth is stable, it can be regarded as a subculture cycle.

[0043] attached by figure 1 It can be seen that with the increase of the number of subcultures, the subculture period is gradually shortened from 63d in the first generation to 44d in the fourth generation, and the growth period is shortened by ...

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Abstract

The invention discloses a tissue culture and rejuvenizing method for adult male populus tomentosa, and belongs to the technical field of vegetative propagation of populus tomentosa. The tissue cultureand rejuvenizing method for the adult male populus tomentosa comprises the following steps: carrying out basic culturing by taking a root coppice shoot stem of male adult superior populus tomentosa at the age of 30-200 as an explant to obtain a sterile seedling which grows well; shearing the sterile seedling into stems with buds with the sizes being 1.0-1.5 cm, inoculating the stems with buds into a proliferation medium and carrying out 2-4-generation subculture to obtain a sterile strong seedling; carrying out rooting culture on the sterile seedling to obtain a sterile bred seedling; and finally, carrying out domestication culture on the sterile bred seedling to obtain a rejuvenized bred seedling to finish tissue culture and rejuvenizing of the adult male populus tomentosa. By the method, rejuvenizing of propagules of the populus tomentosa can be realized, effective induction and regeneration are implemented, mass propagation and forestation can be carried out, meanwhile, excellent properties of seed trees are maintained, the problems of maturation effect of the nursery-grown plants, poor quality of the nursery-grown plants and the like are avoided, and the tissue culture and rejuvenizing method for the adult male populus tomentosa has important guiding significance on production practice.

Description

technical field [0001] The invention belongs to the technical field of asexual reproduction of poplar tomentosa, and in particular relates to a tissue culture juvenile method of adult male poplar tomentosa. Background technique [0002] Poplar is one of the main afforestation tree species through vegetative propagation in my country and even in the world. Populus tomentosa (Populustomentosa) is an excellent timber and landscaping tree species, and is widely cultivated in northern China. Populus tomentosa is a dioecious plant, and the female plant's flying catkins every year has caused great environmental problems. Therefore, it is of great significance to study the reproductive technology of poplar tomentosa. [0003] At present, the common method of asexual reproduction of Populus tomentosa is cutting, but this method is not easy to take root, and the survival rate is unstable. Other methods include burying, burying roots, grafting, etc., but the above-mentioned methods use...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02P60/40Y02A40/22
Inventor 刘勇张劲李国雷贺国鑫薛敦孟邢立霞邵鹏
Owner BEIJING FORESTRY UNIVERSITY
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