Preservation method of lily bulb tissue
A preservation method, lily technology, applied in the direction of plant preservation, botany equipment and methods, applications, etc., can solve the problems affecting the survival rate and preservation effect of plants, and achieve the improvement of recovery growth rate, significant optimization of preservation effect, and air permeability Good results
Inactive Publication Date: 2018-11-02
沿河后花园农业观光旅游综合开发有限公司
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Problems solved by technology
The vitrification method has the advantages of simple and fast operation, low cost, wide variety of suitable preservation materials, stable heredity of preservation materials, and good preservation effect. However, the selection of vitrification solutions and exogenous substances for different plants or cells or tissues will directly affect the quality of the plants. The survival rate and preservation effect of
Method used
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Experimental program
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Effect test
Embodiment
[0031] A method for preserving lily bulb tissue, including the following steps:
[0032] 1) Pre-cultivation: Clean the lily bulbs, slice them, disinfect them, coat them with a layer of mica mixture, and store them in an ice bucket for 0.5 to 4 hours;
[0033] 2) Dehydration: Transfer the lily bulb tissue to a cryotube, treat it with vitrification solution at room temperature for 20 to 35 minutes, and then treat it with PVS2 stock solution at 0°C for 10 to 50 minutes;
[0034] 3) Liquid nitrogen storage: remove the PVS2 stock solution, encapsulate the lily bulb tissue with a wax block, seal it in a freezing tube, and directly put it into liquid nitrogen for cryopreservation;
[0035] 4) Thaw.
[0036] The lily bulb tissue is sealed in liquid nitrogen for 24 hours.
[0037] According to the above steps, the tissues of lily bulbs were divided into experimental group and control group.
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The invention relates to the technical field of plant bulb tissue preservation, in particular to a preservation method of lily bulb tissue. In the pre-culturing process, by coating lily bulb tissue with mica, the stress resistance and survival rate of the lily bulb tissue can be improved; starch and antifreeze glycoprotein are adopted as allogenic materials and added into a vitrified solution to use to prevent lily bulb cytomembrane from damage, and the recovery growth rate of the lily bulb tissue is improved.
Description
Technical field [0001] The invention relates to the technical field of plant bulb tissue preservation, in particular to a method for preserving lily bulb tissue. Background technique [0002] Lily is a herbaceous plant belonging to the genus Lilium in the Liliaceae family. It is a world-famous bulbous flower with the characteristics of rich colors and graceful posture. Lilies are widely used in garden scenery, cut flowers, flowers and potted plants. As the distribution center of lilies, China is rich in germplasm resources, but most wild species are still endangered plants. As a perennial bulbous herb, lily cannot be stored in a low-temperature seed bank. At present, the main storage method is field planting and preservation. Field preservation is prone to browning and rot during the preservation process. Therefore, the medium and long-term preservation problem of lily needs to be solved urgently. [0003] Cryopreservation is a modern in vitro preservation technology for germplas...
Claims
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IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 黄霞
Owner 沿河后花园农业观光旅游综合开发有限公司