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Method for preparing lotus corniculatus feed through microorganism fermentation

A technology for microbial fermentation and alfalfa grass is applied in the directions of animal feed, animal feed, application, etc., which can solve the problems of complicated operation and high cost, and achieve the effect of low cost and easy operation.

Inactive Publication Date: 2018-11-06
何怡江
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This technology has the disadvantages of high cost and cumbersome operation

Method used

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Embodiment 1

[0031] Embodiment 1, a kind of method that microbial fermentation prepares alfalfa forage, carries out following steps successively:

[0032] 1), first crush the alfalfa until it can pass through a 50-mesh sieve;

[0033] Then according to the liquid-to-material ratio of 3ml / g, add the alfalfa and nutrient salt solution after the above pulverization into the container, seal the container mouth and sterilize (sterilize at 121°C for 20min), then cool to room temperature to obtain the raw material solution;

[0034] The preparation method of described nutrient salt solution is: take by weighing 1g KH 2 PO 4 , 2.5g NaCl, 0.5g MgSO 4 ·7H 2 O, 1g (NH 4 ) 2 SO 4 , 0.5g CaCl 2 , mixed, dissolved in water, made up to 1 L, and adjusted to pH 7.0 by adding 2 g / L NaOH solution.

[0035] 2), prepare aspergillus oryzae bacterium liquid, the preparation method of aspergillus oryzae bacterium liquid is to carry out following steps successively:

[0036] ①, strain activation:

[0037...

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Abstract

The invention discloses a method for preparing a lotus corniculatus feed through microorganism fermentation. The method comprises the following steps of (1) adding a nutritive salt solution to crushedlotus corniculatus according to the material-liquid ratio of 1-5ml / g, performing sterilization under the sealed condition, and then performing cooling to room temperature so as to obtain raw materialliquid; (2) according to the inoculum concentration that every 100g of the lotus corniculatus is inoculated with 4-16ml of bacterial liquid, adding aspergillus oryzae liquid of which the spore concentration is 10<6>-10<7> / mL to the raw material liquid, performing placing in a constant-temperature shaking culture box of 26-32 DEG C, and performing fermentation and culturing for 3-10 days; and (3)sterilizing fermentation products obtained in the step (2), then adding water, performing water extraction at 95+ / -10 DEG C for 1+ / -0.5h, wherein the material-liquid ratio of the lotus corniculatus tothe water is 1g to (8-12)ml, performing solid-liquid separation on substances obtained through water extraction, and drying residues so as to obtain the lotus corniculatus feed. Through adoption of the method disclosed by the invention, the protein hydrolysis degree can be increased.

Description

technical field [0001] The invention relates to the field of feed, in particular to a method for preparing alfalfa feed by microbial fermentation. Background technique [0002] Alfalfa (Latin name: Lotus corniculatus L), is a general term for plants of the genus Medicago, native to Europe and America, alfalfa is a perennial flowering plant. The most famous of these is alfalfa (Medicago sativa) used as pasture grass, which is livestock feed. [0003] As the forage with the largest planting area in the world, alfalfa has the advantages of high yield, drought resistance, cold resistance, strong regeneration, high nutritional value, and good palatability. It is an important forage for livestock and poultry. [0004] Alfalfa is known as the "King of Forage Grass". It has high nutritional value, high yield, strong regeneration, and the forage value ranks first among all kinds of pasture. It is an important forage for livestock and poultry, especially for the milk production and l...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/30
CPCA23K10/12A23K10/30
Inventor 何怡江
Owner 何怡江
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