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Tissue repair material with oxygen release ability, preparation method of tissue repair material and application of tissue repair material

A technology for tissue repair and oxygen release, applied in tissue repair materials and its preparation, application in repair and regeneration, in the field of biological oxygen release, can solve problems such as low oxygen release capacity, promote tissue repair, solve uncontrollable and Biotoxicity, the effect of broad clinical application prospects

Active Publication Date: 2018-11-13
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned technical problems in the related art, the present invention provides a tissue repair material with oxygen-releasing ability and its preparation method and application. The problem of low oxygen release capacity can be used to promote the rapid repair of tissue defects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] A tissue repair material with oxygen releasing ability, prepared by the following method:

[0057] (1) Add 0.208g YCl to the three-necked flask 3 , 0.11g YbCl 3 and 0.0078g ErCl 3 After dissolving in a mixed solvent of 6mL oleic acid and 15mL octadecene, add 0.1g NaOH and 0.148g NH 4 After F, the temperature was raised to 300 °C for 1 h under the protection of argon, and the upconversion nanoparticles UCNPs coated with oleic acid were obtained after washing.

[0058] (2) After dissolving 0.05 g of oleic acid-coated UCNPs in toluene, 0.1 g of polylysine in DMSO solution was added, heated to 180 °C for 4 h under the protection of argon, and cooled and washed to obtain water-soluble surface-aminated UCNPs.

[0059] (3) Take 5 mg of surface-aminated UCNPs and redisperse them in 5 mL of MES buffer solution, add 1.5 mg of N-hydroxysuccinimide biotin ester, and react overnight at 4°C to obtain biotinylated UCNPs.

[0060] (4) put 10 5 Disperse photosynthetic bacteria in 5...

Embodiment 2

[0064] A tissue repair material with oxygen releasing ability, prepared by the following method:

[0065] (1) Add 0.208g YCl to the three-necked flask 3 , 0.16g YbCl 3 and 0.0156g TmCl 3 After dissolving in a mixed solvent of 6mL oleic acid and 15mL octadecene, add 0.1g NaOH and 0.148g NH 4 After F, the temperature was raised to 300 °C for 1 h under the protection of argon, and the upconversion nanoparticles UCNPs coated with oleic acid were obtained after washing.

[0066] (2) After dissolving 0.05 g of oleic acid-coated UCNPs in toluene, 0.1 g of polyethylenediamine in DMSO solution was added, heated to 180 °C for 4 h under the protection of argon, and cooled and washed to obtain water-soluble surface-aminated UCNPs.

[0067] (3) Take 5 mg of surface-aminated UCNPs and redisperse them in 5 mL of MES buffer solution, add 1.5 mg of N-hydroxysuccinimide biotin ester, and react overnight at 4°C to obtain biotinylated UCNPs.

[0068] (4) put 10 5 Disperse photosynthetic bact...

Embodiment 3

[0072] A tissue repair material with oxygen releasing ability, prepared by the following method:

[0073] (1) Add 0.208g YCl to the three-necked flask 3 , 0.22g YbCl 3 and 0.0078g ErCl 3 After dissolving in a mixed solvent of 6mL oleic acid and 15mL octadecene, add 0.1g NaOH and 0.148g NH 4 After F, the temperature was raised to 300 °C for 1 h under the protection of argon, and the upconversion nanoparticles UCNPs coated with oleic acid were obtained after washing.

[0074] (2) After dissolving 0.05 g of oleic acid-coated UCNPs in toluene, 0.1 g of polychitosan in DMSO solution was added, heated to 180 °C for 4 h under the protection of argon, and water-soluble surface-aminated UCNPs were obtained after cooling and washing.

[0075] (3) Take 5 mg of surface-aminated UCNPs and redisperse them in 5 mL of MES buffer solution, add 1.5 mg of N-hydroxysuccinimide biotin ester, and react overnight at 4°C to obtain biotinylated UCNPs.

[0076] (4) put 10 5 Disperse photosynthetic...

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PUM

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Abstract

The invention provides a tissue repair material with the oxygen release ability, a preparation method of the tissue repair material and an application of the tissue repair material. The tissue repairmaterial comprises oxygen release particles and organic polymers, the oxygen release particles upconversion nano-particles and photosynthetic bacteria which are conjugated. The preparation method of the tissue repair material includes the steps: firstly, preparing the upconversion nano-particles; modifying surfaces of the upconversion nano-particles; conjugating the upconversion nano-particles onthe surfaces of the photosynthetic bacteria to form bio-conjugates; loading the bio-conjugates through bio-macromolecules to prepare the tissue repair material. The tissue repair material has an in-situ oxygen release function under irradiation of infrared light, so that early tissue repair is facilitated, and the tissue repair material solves the problem that conventional chemical oxygen releaseis uncontrollable and has biotoxicity. The tissue repair material is a porous tissue engineering support material which can efficiently facilitate tissue repair, and the tissue repair material has a wide clinical application prospect.

Description

technical field [0001] The present invention relates to the technical field of biomedical materials, in particular, to a tissue repair material with oxygen-releasing ability and its preparation method and application, especially to a biological oxygen-releasing material through the synergistic effect of upconversion nanoparticles and photosynthetic bacteria A tissue repair material, a preparation method thereof, and an application of the tissue repair material in the repair and regeneration of bone / cartilage defects. Background technique [0002] The repair and regeneration of bone / cartilage defects has always been a major clinical problem. Although bone tissue has a certain ability to regenerate, large-volume bone defects cannot achieve self-repair and still require the implantation of bone repair materials. Millions of cases of bone grafting are needed every year in the world, resulting in a huge gap in clinical demand for implant materials. Therefore, using the principl...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/04A61L27/22A61L27/24A61L27/20A61L27/50A61L27/56
CPCA61L27/047A61L27/20A61L27/222A61L27/24A61L27/3637A61L27/365A61L27/3654A61L27/50A61L27/56A61L2300/412A61L2430/02A61L2430/06C08L5/08C08L1/02
Inventor 谢辉刘浩明殷豪
Owner XIANGYA HOSPITAL CENT SOUTH UNIV