Low-temperature degradation straw composite microbial agent containing Saccharomyces cerevisiae and application thereof

A compound bacterial agent and lactic acid bacteria technology, applied in the field of microbiology, can solve the problems of short planting time of farmers in autumn plowing, blockage of rivers and road traffic, and market information blockage, so as to improve food digestibility and biological value, and maintain micro-ecological balance , the effect of increasing appetite

Active Publication Date: 2018-11-13
JILIN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the improvement of crop yields, the total amount of straw has increased rapidly. In order to catch up with the farming season, rush to sow seeds, and save trouble, the majority of farmers have begun to focus on the phenomenon of straw burning in most areas. A headache. In the past, due to the lack of development and utilization technology, market information blockage, and the short time for farmers to sow in autumn, most of the straw was discarded in the river,

Method used

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  • Low-temperature degradation straw composite microbial agent containing Saccharomyces cerevisiae and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The mutagenic screening of embodiment 1 verrucous Myromyces

[0021] (1) Morphological identification and screening

[0022] The soil in the forest area of ​​Changbai Mountain Nature Reserve was collected and cultivated on PDA medium by conventional methods. The mycelium of the strain was white flocculent at first, and grew divergently towards the periphery. After 8 days of culture, the color of conidia continued to deepen, and glue dots appeared; after 10 days of culture, the colony was in the shape of concentric rings, the conidia showed black glue spots, and the back of the colony appeared Hazel radial folds.

[0023] Use a puncher to get a 1cm-diameter bacterial block, inoculate it on an aniline blue selective medium, and culture it in an aerobic incubator at 30°C for 10 days, observe the fading of aniline blue, and select a strain with a fast fading rate and strong ability as candidate strains.

[0024] At the same time, in order to directional screen lignin-deg...

Embodiment 2

[0030] The low-temperature acclimatization of embodiment 2 bacterial strains

[0031] (1) Enrichment culture strains

[0032] Inoculate the strain into a Erlenmeyer flask filled with 150mL of PDA medium, add filter paper pieces with a length of 5cm and a width of 2cm, incubate at a constant temperature at 30°C for 1 hour and mix evenly, and make 10 -1 -10 -9 For dilution, 1 mL of each dilution was inoculated in PDA medium with filter paper as the only carbon source, and static enrichment culture was carried out at 28°C for 4 generations. Screen out the materials with short breakage time and high degree of ulceration of filter paper. PDA medium: potato 200g / L, glucose 20g / L, agar 15g / L, enrichment culture for 4 generations.

[0033] (2) Preliminary screening and domestication of low-temperature cellulose-degrading composite strains

[0034] Using the filter paper disintegration method, the above-mentioned enriched culture was inoculated into the filter paper strip medium to...

Embodiment 3

[0044] The preparation of embodiment 3 composite bacterial agents

[0045] Cellulase-producing Paenibacillus, xylanase-producing Alternaria, laccase-producing Mycobacterium verrucosus, lignin-peroxidase-producing Bacillus colloid, manganese-peroxidase-producing Lys Bacteria and lactic acid bacteria were activated in a conventional manner and cultured until the number of viable bacteria in the bacterial solution reached 2.0×10 8 individual / mL.

[0046] Mix the bacterial solution according to the following mass ratio: 20% of Paenibacillus cellulase-producing bacteria, 15% of Alternaria xylanase-producing bacteria, 20% of Laccase-producing Mycobacterium verrucosus, lignin peroxidase glue 10% Bacillus plasmogenus, 10% manganese peroxidase-producing Laysia actinomycetes, 25% lactic acid bacteria, fully stirred and mixed to obtain a mixed bacterial liquid.

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Abstract

The invention provides a low-temperature degradation straw composite microbial agent containing Saccharomyces cerevisiae. The composite bacterial agent uses 6 strains, so that the corn stalk maturitydays can be increased by 2 to 3 days in an environment with the outdoor temperature of 1 to 6 DEG C, and then the composite bacterial agent is particularly suitable for northern regions. Compared withthe control group, the average milk yield of cows fed with corn straw treated with compound bacteria is increased by 8.9kg, the content of crude fibers in cow manure is decreased by 3.4%, and the average daily intake is increased by 2.3kg, because the compound bacteria for degrading lignocelluloses is added, and a large amount of Saccharomyces cerevisiae is contained in the compound bacteria, thecorn straw has soft texture and acid aroma, and the palatability is obviously improved to increase the appetite of livestock. Furthermore, the Saccharomyces cerevisiae can promote animal growth, regulate normal gastrointestinal flora and maintain microecological balance, thereby having the effects of improving gastrointestinal functions, increasing food digestibility and biological values, reducing serum cholesterol, controlling endotoxin, inhibiting the growth of spoilage bacteria in intestinal tracts and improving the immunity of bodies and the like so as to further increase the daily output.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a low-temperature straw-degrading straw composite bacterial agent containing lactic acid bacteria and its application. Background technique [0002] With the improvement of crop yields, the total amount of straw has increased rapidly. In order to catch up with the farming season, rush to sow seeds, and save trouble, the majority of farmers have begun to focus on the phenomenon of straw burning in most areas. A headache. In the past, due to the lack of development and utilization technology, market information blockage, and the short time for farmers to sow in autumn, most of the straw was discarded in the river, rotted by the roadside, or directly burned in the field, which not only blocked the river and road traffic. Moreover, it seriously pollutes the environment and causes a huge waste of resources. [0003] In recent years, with the continuous deepening of lignocellulose biodeg...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14C05F15/00C05F17/00A23K50/10A23K10/12C12R1/07C12R1/865C12R1/645C12R1/01
CPCA23K10/12A23K50/10C05F11/00C05F17/00C12N1/14C12N1/18C12N1/20C05F11/08Y02W30/40
Inventor 孙旸陈光地里拜尔努尔孙春玉高祎妍张斯童苏瑛杰苟泽昌地里拜尔 努尔
Owner JILIN AGRICULTURAL UNIV
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