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Decomposition agent for composting and preparation method and application thereof

A decomposing agent and composting technology, which is applied in the field of microbial fermentation, can solve the problems of uneven quality and high product prices, and achieve the effects of a comprehensive combination of bacteria agents, saving water and electricity costs, and a high number of effective viable bacteria

Inactive Publication Date: 2018-11-13
湖南慧谷农业生态研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many kinds of decomposing agents on the market, the quality of which is uneven, and the price of products with better quality is relatively expensive. Therefore, it is more meaningful to develop a decomposing agent with good decomposing effect and low price.

Method used

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  • Decomposition agent for composting and preparation method and application thereof
  • Decomposition agent for composting and preparation method and application thereof
  • Decomposition agent for composting and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The application relates to a decomposing agent for composting, the preparation method of which is as follows:

[0054] The number of Bacillus amyloliquefaciens is ACCC19745, the number of Bacillus subtilis is ACCC19374, the number of Trichoderma harzianum is ACCC30371, the number of lactic acid bacteria is ACCC10295, and the number of Saccharomyces cerevisiae is ACCC20251.

[0055] 1) Bacillus mixed fermentation

[0056] a. Bacillus amyloliquefaciens and Bacillus subtilis were activated and cultivated on nutrient agar medium for 36 hours;

[0057] b. Bacillus amyloliquefaciens and Bacillus subtilis were inoculated into LB medium (tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1L, pH7.0-7.5), at 160r / min, 35 Cultivate at ℃ for 18 hours to obtain the shake flask seed liquid; inoculate the shake flask seed liquid with an inoculation amount of 3% by volume to the fermenter for cultivation, and the culture medium of the fermenter is as follows: the cul...

Embodiment 2

[0079] The application relates to a decomposing agent for composting, wherein the Bacillus mixed bacteria fermentation medium is:

[0080] 97.8 parts of bran, 1 part of glucose, 0.5 parts of soybean meal, 0.5 parts of NaH2PO; 0.1 parts of MnSO4, 0.1 parts of MgSO4;

[0081] Its preparation method is the same as that of Example 1, and the fermentation time of bacillus mixed bacteria is 5 days.

Embodiment 3

[0083] The application relates to a decomposing agent for compost, wherein the Bacillus mixed bacteria fermentation medium is:

[0084] 90.5 parts of bran, 3 parts of glucose, 3.5 parts of soybean meal, 42 parts of NaH2PO; 0.5 parts of MnSO4, 0.5 parts of MgSO4;

[0085] Its preparation method is the same as that of Example 1, and the fermentation time of bacillus mixed bacteria is 4 days.

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Abstract

The invention relates to a decomposition agent for composting and a preparation method and application thereof. The preparation method comprises the steps that S1, a bacillus mixed fermentation dryeris prepared; S2, a trichoderma harzianum, brewer's yeast and lactic acid bacteria mixed dryer is prepared; S3, a decomposition agent is compounded, the bacillus mixed fermentation dryer prepared in the step S1 and the trichoderma harzianum, brewer's yeast and lactic acid bacteria mixed dryer prepared in the step S2 are mixed evenly according to the proportion to obtain the decomposition agent. Thedecomposition agent has the advantages that the decomposition agent is produced by adopting the method of adopting bacillus playing a main role for mixed fermentation, a selected culture medium doesnot need sterilization treatment, the living bacteria count of the bacillus mixture obtained through mixed fermentation can reach 20 billion cfu / g or above. The decomposition agent is mainly applied to composting fermentation excrements of livestock, dreg substances, straw and the like.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a decomposing agent for compost and its preparation method and application. Background technique [0002] my country is a large agricultural country, and a large amount of agricultural and animal husbandry waste is produced every year. my country is rich in straw resources, with an annual output of 384 million tons in 1980, and 535 million tons in 1990. In 2012, the annual output of straw resources reached 707 million tons, and the annual increase trend is maintained according to statistics. In addition, according to statistics, the physical volume of the main livestock and poultry manure resources in my country exceeds 4.7 billion tons. At present, about 2.4 billion tons of livestock and poultry manure in my country have not been treated as resources. These resources that have not been well utilized have become a potential hidden danger to the protection of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F17/00C12R1/125C12R1/07C12R1/865C12R1/01C12R1/885
CPCC05F17/20C12N1/14C12N1/16C12N1/20Y02W30/40
Inventor 谭石勇罗志威谭武贵郭帅郑双凤聂勇
Owner 湖南慧谷农业生态研究院有限公司
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