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Kit for detecting polymorphism of OCA2 (Oculocutaneous Albinism II) gene

A gene polymorphism and kit technology, used in recombinant DNA technology, microbial determination/test, DNA/RNA fragments, etc. Effect

Inactive Publication Date: 2018-11-13
无锡正则精准医学检验有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defect that the method for detecting OCA2 gene polymorphism in the prior art is complicated to operate, and a kind of test kit for detecting OCA2 gene polymorphism is provided

Method used

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  • Kit for detecting polymorphism of OCA2 (Oculocutaneous Albinism II) gene
  • Kit for detecting polymorphism of OCA2 (Oculocutaneous Albinism II) gene
  • Kit for detecting polymorphism of OCA2 (Oculocutaneous Albinism II) gene

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Embodiment

[0040] A kit for detecting polymorphisms of the OCA2 gene, including a total of 0.4 μL of specific primers for detecting the rs1800414 site on the OCA2 gene at a concentration of 10 μM, of which 0.2 μL each for the forward primer and reverse primer; the concentration is 5 μM for detection A total of 0.2 μL of specific fluorescent probes for the rs1800414 site on the OCA2 gene, including 0.1 μL of wild-type probes and 0.1 μL of mutant probes; a total of 0.4 μL of specific primers for the detection site of the internal reference gene β-actin at a concentration of 10 μM , wherein the sense primer and antisense primer are 0.2 μL each; the concentration is 5 μM of the specific fluorescent probe for the detection site of the internal reference gene β-actin, a total of 0.2 μL; the concentration is 5 units / μl of Taq DNA polymerase 0.2 μL; PCR reaction Buffer 15 μL, including MgCl2: 25 mmol / L; dNTPs (dATP, dCTP, dGTP, dTTP): 20 mmol / L; deionized water 20 μL.

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Abstract

The invention discloses a kit for detecting the polymorphism of an OCA2 (Oculocutaneous Albinism II) gene. The kit is prepared from a specific primer and a specific fluorescent probe which are used for detecting an No.rs1800414 locus on the OCA2 gene, a specific primer and the specific fluorescent probe on detection loci of a reference gene beta-actin, a positive quality control product, a negative quality control product, Taq DNA (Deoxyribonucleic Acid) polymerase, a PCR (Polymerase Chain Reaction) buffer solution and the like. The kit disclosed by the invention can be used for detecting thepolymorphism of the OCA2 gene, detection primers and probes can be designed aiming at specific SNP (Single Nucleotide Polymorphism) loci of the OCA2 gene, the sensitivity is high, and the specificityis strong; a detection object is derived from an oral swab, and the whole detection process is in non-invasive detection; a new kit is provided for detecting the polymorphism of the OCA2 gene, and theapplication is convenient.

Description

technical field [0001] The invention relates to a kit for detecting OCA2 gene polymorphism. Background technique [0002] Hyperpigmentation is the pigmentation change of human skin in different colors, ranges and shades due to various reasons. Variations in pigmentation are caused by biochemical differences that affect the number of melanosomes produced, the type of melanin, and the size and shape of the melanosomes. The cause of skin hyperpigmentation is usually the formation of black skin pigmentation after being stimulated by sunlight, ultraviolet radiation or cosmetics, drugs and other substances. Skin pigmentation can be divided into grade I as mild photoaging, with no visible plain pigmentation, and no need to use concealer to cover the spots; grade II as moderate photoaging, with more and more obvious pigmentation, Brown spots, uneven skin tone, freckles, etc. appear; Grade III is high degree of photoaging. At this time, the pigmentation problem is aggravated, and ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 刘小曼姚鲁帅王莎
Owner 无锡正则精准医学检验有限公司
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