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Preparation method of poultry plasma protein

A technology of plasma protein and plasma protein powder, which is applied in the field of plasma protein processing, can solve the problems of limiting the industrialization and application range of plasma protein, poor flexibility of plasma protein structure, and reduced overall solubility, so as to improve the overall solubility and reduce particle size. The effect of small and soft shaking conditions

Active Publication Date: 2018-11-16
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the tight and highly folded molecular structure of plasma protein, the flexibility of plasma protein structure is poor, and the overall solubility is correspondingly reduced. Furthermore, due to the poor dissolution environment, the functional properties such as emulsification and foaming properties are affected, which greatly limits plasma protein. Industrialization promotion and application scope
So far, most studies on plasma proteins are still focused on direct utilization, and there is a lack of in-depth research on improving the functional properties of meat products.

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  • Preparation method of poultry plasma protein

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preparation example Construction

[0032] The invention provides a method for preparing poultry plasma protein, comprising the steps of:

[0033] Step 1, taking poultry blood, and obtaining plasma liquid through processing;

[0034] Step 2: Perform ultrasonic treatment on the plasma liquid, the ultrasonic power is 100-300W, and the ultrasonic time is 10-30min;

[0035] Step 3: Adjust the pH of the ultrasonically treated plasma to 8.5-9.5, and then perform ultrafiltration at an ultrafiltration pressure of 0.3-0.6 MPa, with a molecular weight cut-off of 5,000-10,000 Da of plasma, and an ultrafiltration cycle time of 10-100 Da. 30min;

[0036] Step 4, drying the plasma liquid obtained in Step 3 to obtain poultry plasma protein powder.

[0037] The invention conducts ultrasonic treatment on plasma liquid, which can effectively destroy the highly folded and compact structure of plasma protein molecules, change the flexibility of plasma protein structure, improve its overall solubility, have good emulsification and...

Embodiment 1

[0055] A method for preparing poultry plasma protein, comprising the steps of:

[0056] (1) Blood collection: fresh and healthy poultry blood is collected with a vacuum blood collection knife, and a compound anticoagulant is added. The final concentration of the agent in the blood is 0.8g / mL, mix well, and store under refrigerated conditions.

[0057] (2) Separation at rest: the blood was left at rest at 5° C., and centrifuged at 2500 g for 15 minutes to obtain the upper layer of plasma.

[0058] (3) Ultrasonic treatment is performed on the upper plasma liquid mixture, the ultrasonic power is 200W, and the ultrasonic time is 20min;

[0059] (4) Use NaOH / NaHCO 3 Adjust the pH of plasma liquid to 9.0, 0.45MPa ultrafiltration to the end, desalination, plasma liquid with a molecular weight cut-off of 7500Da, ultrafiltration cycle time is 20min.

[0060] (5) Drying: Pre-freeze the treated plasma liquid at -35°C, and freeze-dry at -45°C for 60 hours in a cold trap to obtain poult...

Embodiment 2

[0062] A method for preparing poultry plasma protein, comprising the steps of:

[0063] (1) Blood collection: fresh and healthy poultry blood is collected with a vacuum blood sampling knife, and a compound anticoagulant is added, which includes sodium citrate, sodium citrate, and heparin with a mass ratio of 1:1:1, so that The final concentration of the agent in the blood is 0.6g / mL, mix well, and store under refrigerated conditions.

[0064] (2) Static separation: the blood was allowed to stand at 4°C, and centrifuged at 2000g for 10 minutes to obtain the upper layer of plasma.

[0065] (3) Ultrasonic treatment is performed on the upper plasma liquid mixture, the ultrasonic power is 100W, and the ultrasonic time is 10min;

[0066] (4) Use NaOH / NaHCO 3 Adjust the pH of the plasma solution to 8.5, 0.3MPa ultrafiltration to the end, desalination, the plasma solution with a molecular weight cut-off of 5000Da, and an ultrafiltration cycle time of 10 minutes.

[0067] (5) Drying...

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Abstract

The invention discloses a preparation method of poultry plasma protein. The preparation method comprises the following steps of step I, taking poultry blood, and performing treatment to obtain plasmalipid; step II, performing ultrasonic treatment on the plasma lipid, wherein the power for ultrasonic treatment is 100-300W, and the time for ultrasonic treatment is 10-30min; step III, regulating thepH of the plasma lipid after ultrasonic treatment to 8.5-9.5, then performing ultrafiltration under the ultrafiltration pressure of 0.3-0.6MPa, cutting off the plasma lipid of which the molecule is 5000-10000Da, wherein the ultrafiltration circulation time is 10-30min; and step IV, drying the plasma lipid obtained in the step III, so as to obtain the poultry plasma protein powder. The solubilityof the poultry plasma protein prepared by the method disclosed by the invention is increased to 85-88% from 50-56%. The plasma protein prepared by the method has better antioxidant activity.

Description

technical field [0001] The invention belongs to the technical field of plasma protein processing and relates to a preparation method of poultry plasma protein. Background technique [0002] Animal blood is a by-product of the slaughtering and processing industry of livestock and poultry. It is rich in protein, contains a variety of nutrients and biologically active components, and is known as "liquid meat". As the world's largest meat production country, my country is rich in poultry and livestock blood resources, with an annual output of about 400×10 4 t, but the waste of livestock and poultry blood resources in our country is very serious. Especially in my country's poultry slaughtering enterprises, about 90% of them basically do not use them effectively. The direct discharge of blood not only causes waste of valuable protein resources, but also brings serious environmental pollution. [0003] Plasma protein powder is a product obtained by using the blood of slaughtered ...

Claims

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Application Information

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IPC IPC(8): A23J1/06A23J3/00
CPCA23J1/06A23J3/00
Inventor 邹烨王道营李鹏鹏张新笑杨恒卞欢吴海虹诸永志徐为民
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES