Double-recognition locus glycoprotein surface imprinting nano material as well as preparation method and application thereof

A nanomaterial, surface imprinting technology, applied in chemical instruments and methods, other chemical processes, etc., can solve the problems of poor adsorption effect of boron affinity recognition, cumbersome preparation process, etc., to achieve selective affinity recognition and easy raw materials. Obtain and improve the effect of weak binding

Active Publication Date: 2018-11-16
合肥九州龙腾科技成果转化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the cumbersome preparation process of boron affinity surface imprinted adsorbents in the prior art, and the defects of poor adsorption effect of single boron affinity recognition, and provide a double recognition site glycoprotein surface imprinted nanomaterial and its preparation method

Method used

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  • Double-recognition locus glycoprotein surface imprinting nano material as well as preparation method and application thereof
  • Double-recognition locus glycoprotein surface imprinting nano material as well as preparation method and application thereof
  • Double-recognition locus glycoprotein surface imprinting nano material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Preparation of polyglycidyl methacrylate (PSG) nanoparticle dispersion:

[0047] Add 1.5 mL of GMA, 0.05 mL of DVB and 0.2 mL of St into a 250 mL three-neck flask, then add 100 mL of double-distilled water and insert a thermometer and a nitrogen tube, and continue stirring and heating under a nitrogen atmosphere. When the temperature reached 71 °C, 1.5 mL of 0.03 g / mL potassium persulfate solution was added to initiate polymerization, and the reaction was continued for 6.0 h. After the reaction, the product was separated by centrifugation, washed three times with ethanol and double-distilled water, and then dispersed in 100 mL of double-distilled water to obtain a polyglycidyl methacrylate (PSG) nanoparticle dispersion for later use.

[0048] (2) Surface chelated Cu 2+ Polyglycidyl methacrylate nanoparticles (PSG / IDA-Cu 2+ ) preparation:

[0049] Add 1.0 g IDA and 1.0 g NaOH to a 250 mL three-necked flask, and then add 100 mL of the polyglycidyl methacrylate (PSG...

Embodiment 2

[0057] (1) Preparation of polyglycidyl methacrylate (PSG) nanoparticle dispersion:

[0058] Add 2.348 mL of GMA, 0.064 mL of DVB and 0.46 mL of St into a 250 mL three-neck flask, then add 100 mL of double-distilled water, insert a thermometer and a nitrogen tube, and continue stirring and heating under a nitrogen atmosphere. When the temperature reached 71 °C, 2.0 mL of 0.03 g / mL potassium persulfate solution was added to initiate polymerization, and the reaction was carried out for 8.0 h. After the reaction, the product was separated by centrifugation, and the product was washed three times with ethanol and double distilled water, and then the product was dispersed in 100 mL double distilled water, separated, and the product was washed three times with ethanol and double distilled water, and then the product Disperse in 100 mL double-distilled water to obtain a polyglycidyl methacrylate (PSG) nanoparticle dispersion, which is set aside.

[0059] (2) Surface chelated Cu 2+ P...

Embodiment 3

[0064] (1) Preparation of polyglycidyl methacrylate (PSG) nanoparticle dispersion:

[0065] Add 3.0 mL of GMA, 1.0 mL of DVB and 0.6 mL of St into a 250 mL three-necked flask, then add 100 mL of double-distilled water and insert a thermometer and a nitrogen tube, and continue stirring and heating under a nitrogen atmosphere. When the temperature reached 71 °C, 2.5 mL of 0.03 g / mL potassium persulfate solution was added to initiate polymerization, and the reaction was carried out for 9.0 h. After the reaction, the product was separated by centrifugation, washed three times with ethanol and double distilled water, and then the product was dispersed in 100 mL double distilled water to obtain a polyglycidyl methacrylate (PSG) nanoparticle dispersion for future use.

[0066] (2) Surface chelated Cu 2+ Polyglycidyl methacrylate nanoparticles (PSG / IDA-Cu 2+ ) preparation:

[0067] 2.5 g IDA and 1.5 g NaOH were added to a 250 mL three-neck flask, followed by 100 mL of the polyglyci...

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Abstract

The invention relates to a double-recognition locus glycoprotein surface imprinting nano material as well as a preparation method and application thereof, and belongs to the technical field of preparation of medical functional materials. The method comprises the following steps that firstly, iminodiacetic acid is modified on the surfaces of poly glycidyl methacrylate nanoparticles with epoxy bonds, copper ions are chelated to form metal ion affinity loci, secondly, PSG nanoparticles modified with the metal ion affinity loci are co-cultured with template glycoprotein molecular OVA, template protein is fixed on the surface of the particles, oxidative initiation is carried out on water-soluble 3-aminobenzeneboronic acid to undergo polymerization, then a series of treatment is carried out so as to obtain the double-recognition locus glycoprotein surface imprinting nano material, and the material is used for selective recognition and separation of glycoprotein. According to the nano material as well as the preparation method and application thereof, the preparation process is simple, convenient and feasible, the synthesis cost is effectively lowered, the double-recognition loci enable PSG-MIPs to have stronger adsorption affinity for the glycoprotein, and therefore the selective adsorption capacity of the glycoprotein is effectively enhanced.

Description

technical field [0001] The invention relates to a preparation method of surface imprinted nanomaterials, in particular to a double recognition site glycoprotein surface imprinted nanomaterial and its preparation method and application, belonging to the technical field of preparation of medical functional materials. Background technique [0002] Glycoproteins have been proven to be closely related to many diseases, such as diabetes, cancer and immune disorders. At present, glycoproteins are often used as diagnostic and therapeutic markers in clinical treatment. Therefore, capturing and detecting glycoproteins from complex biological samples has extremely high medical value. [0003] The boron affinity method is widely used in the separation of glycoproteins because of its reversible covalent interaction with cis-dihydroxy compounds. In an alkaline environment, boric acid forms molecular covalent bonds with glycoproteins to form five- or six-membered boronic acid cyclic este...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/30C08F8/42C08F8/30C08F220/32C08F212/36C08F212/08
CPCB01J20/268B01J20/30B01J2220/445C08F8/42C08F220/32C08F220/325C08F212/36C08F212/08C08F8/30
Inventor 潘建明朱恒佳刘金鑫夏可旭
Owner 合肥九州龙腾科技成果转化有限公司
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