Method for promoting germination of rhizoma polygonati seeds
A technology of seeds and Polygonatum, which is applied in the fields of seed and rhizome treatment, application, agriculture, etc., can solve the problems of ineffective use of seeds, unfavorable promotion of Polygonatum planting, waste of commercial medicinal materials, etc., and achieve fast speed, well-developed root system, and permeability enhanced effect
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Embodiment 1
[0016] A method for promoting the germination of sealwort seeds, comprising the following steps:
[0017] (1) Seed collection: collect the sealwort seeds of natural drying;
[0018] (2) Seed disinfection treatment: soaking Polygonatum seeds with a concentration of 40g / L sodium percarbonate solution for 30min;
[0019] (3) Prepare seed soaking solution: select well-developed alfalfa grass, weigh 30g after crushing, add 50mL distilled water, seal with parafilm, place at 25°C, 120r min -1 Shaking table for 24 hours, ultrasonic extraction for 15 minutes; after the crude extract was filtered, the residue was extracted twice according to the above method; all the filtrates were combined, concentrated under reduced pressure on a rotary evaporator at 40°C, and finally settled to a volume of 50 mL; Add 16ppm gibberellin and 12ppm kinetin to obtain soaking liquid;
[0020] (4) dry heat treatment: the sterilized sealwort seeds were dry heat treated at 50° C. for 60 min;
[0021] (5) S...
Embodiment 2
[0023] A method for promoting the germination of sealwort seeds, comprising the following steps:
[0024] (1) Seed collection: collect the sealwort seeds of natural drying;
[0025] (2) Seed disinfection treatment: soak the Polygonatum seeds with a concentration of 50g / L sodium percarbonate solution for 35min;
[0026] (3) Prepare seed soaking solution: select well-developed alfalfa grass, weigh 30g after crushing, add 50mL distilled water, seal with parafilm, place at 25°C, 120r min -1 Shaking table for 24 hours, ultrasonic extraction for 15 minutes; after the crude extract was filtered, the residue was extracted twice according to the above method; all the filtrates were combined, concentrated under reduced pressure on a rotary evaporator at 40°C, and finally settled to a volume of 50 mL; Add 16ppm gibberellin and 12ppm kinetin to obtain soaking liquid;
[0027] (4) dry heat treatment: Dry heat treatment of the sterilized Polygonatum seeds at 53°C for 70 minutes;
[0028]...
Embodiment 3
[0030] A method for promoting the germination of sealwort seeds, comprising the following steps:
[0031] (1) Seed collection: collect the sealwort seeds of natural drying;
[0032] (2) Seed disinfection treatment: soak the Polygonatum seeds with a concentration of 60g / L sodium percarbonate solution for 40min;
[0033] (3) Prepare seed soaking solution: select well-developed alfalfa grass, weigh 30g after crushing, add 50mL distilled water, seal with parafilm, place at 25°C, 120r min -1 Shaking table for 24 hours, ultrasonic extraction for 15 minutes; after the crude extract was filtered, the residue was extracted twice according to the above method; all the filtrates were combined, concentrated under reduced pressure on a rotary evaporator at 40°C, and finally settled to a volume of 50 mL; Add 16ppm gibberellin and 12ppm kinetin to obtain soaking liquid;
[0034] (4) dry heat treatment: dry heat treatment of the sterilized Polygonatum seeds at 55°C for 80 minutes;
[0035]...
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