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Adipose tissue digestive juice and method for rapidly obtaining SVF (stromal vascular fraction) cell

A technology of stromal vascular components and adipose tissue, applied in the field of cell separation, can solve the problems of long time and low viability, and achieve the effect of simple formula

Inactive Publication Date: 2018-11-20
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the routine acquisition of stromal vascular component cells takes a long time, and the viability is low

Method used

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  • Adipose tissue digestive juice and method for rapidly obtaining SVF (stromal vascular fraction) cell
  • Adipose tissue digestive juice and method for rapidly obtaining SVF (stromal vascular fraction) cell
  • Adipose tissue digestive juice and method for rapidly obtaining SVF (stromal vascular fraction) cell

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] 1. The digestive formula of this embodiment is:

[0057] Type I collagenase: 0.25%;

[0058] HEPES buffer: 5mM;

[0059] DMEM / F12 medium: make up.

[0060] The above raw materials are all sterile.

[0061] 2. Digestion method:

[0062] Wash the adipose tissue until no red blood cells are visible to the naked eye, shred it to about 30 cubic millimeters, and digest it with the above-mentioned digestive solution for 20 minutes at 37°C and shake at 150rpm.

Embodiment 2

[0064] 1. The digestive formula of this embodiment is:

[0065] Type I collagenase: 0.25%;

[0066] HEPES buffer: 10mM;

[0067] DMEM / F12 medium: make up.

[0068] The above raw materials are all sterile.

[0069] 2. Digestion method:

[0070] Wash the adipose tissue until no red blood cells are visible to the naked eye, shred it to about 30 cubic millimeters, and digest it with the above-mentioned digestive solution for 20 minutes at 37°C and shake at 150rpm.

Embodiment 3

[0072] 1. The digestive formula of this embodiment is:

[0073] Type I collagenase: 0.25%;

[0074] HEPES buffer: 15mM;

[0075] DMEM / F12 medium: make up.

[0076] The above raw materials are all sterile.

[0077] 2. Digestion method:

[0078] Wash the adipose tissue until no red blood cells are visible to the naked eye, cut it to about 30 cubic millimeters, and digest it with the above-mentioned digestive solution for 20 minutes at 37°C and shake at 150rpm.

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Abstract

The invention relates to the technical field of cell separation, in particular to adipose tissue digestive juice and a method for rapidly obtaining an SVF (stromal vascular fraction) cell. The adiposetissue digestive juice comprises the following ingredients: I-type collagenase, HEPES buffer solution and a DEMEM / F12 culture medium. According to the adipose tissue digestive juice provided by the invention, the pH value of a digestive system can be stably maintained between 6.8-8.2, so that the activity of the I-type collagenase is optimal. Therefore, complete digestion can be realized in a short time. The time for obtaining the SVF according to the traditional digestive scheme is 40 min, while the SVF with high quality and high quantity can be obtained within 15-20 min in such a way that the digestive juice is adopted for digesting adipose tissues.

Description

technical field [0001] The invention relates to the technical field of cell separation, in particular to adipose tissue digestion fluid and a method for rapidly obtaining stromal blood vessel component cells. Background technique [0002] Refractory wounds are a common disease among surgical diseases. They have the characteristics of long course of disease, many complications, and great impact on appearance. In recent years, they have been increasing year by year and have become a factor that seriously affects the quality of life of patients. How to promote the healing of refractory wounds has been explored, but so far there is still no ideal method. Mesenchymal stem cells (MSCs), first discovered in the bone marrow, are cells with multilineage differentiation potential and have the potential to promote tissue regeneration. MSCs can accelerate wound healing by promoting angiogenesis and tissue regeneration in local tissues. However, due to the low content of MSCs in bone m...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/077C12N5/071C12N5/0786
CPCC12N5/0645C12N5/0653C12N5/0656C12N5/0667C12N5/069C12N2509/00
Inventor 王一飞陈海佳葛啸虎黄幸王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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