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A rapid detection method for drugs in hair

A detection method and hair technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome operation, long time-consuming, high cost, etc., and achieve the effects of high detection sensitivity, reduced interference, and light weight

Active Publication Date: 2021-06-08
浙江诺迦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above chromatographic methods have good sensitivity and specificity, the operation is cumbersome and time-consuming, requiring expensive equipment and professional operators
In recent years, enzyme-linked immunosorbent assay (ELISA) has also been applied to the detection of drugs in hair digestion solution, but there are also problems such as cumbersome operation, long time-consuming, and high cost.

Method used

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  • A rapid detection method for drugs in hair
  • A rapid detection method for drugs in hair
  • A rapid detection method for drugs in hair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1: Hair Sample Morphine (MOP) Detection

[0073] Due to the large molecular weight of the colloidal gold-labeled morphine antibody, after it combines with the morphine-BSA probe on the surface of the SPR sensor biochip, the SPR response value changes greatly. If the hair sample contains morphine, it binds to the colloidal gold-labeled morphine antibody in the buffer, resulting in a small number of antibodies bound to the morphine-BSA probe on the chip surface, small changes in the refractive index of the chip medium and SPR resonance peaks, and a decrease in the SPR response value. Therefore, the concentration of morphine molecules in the hair sample is inversely proportional to the change in the SPR response.

[0074] The results of the morphine channel test showed that the 200-fold diluted morphine gold-labeled antibody methyl SPR response value was 24, and the 200-fold diluted morphine gold-labeled antibody and the handheld colloidal gold optical module intel...

Embodiment 2

[0075] Example 2 Detection of methamphetamine (MET) in hair samples

[0076] Due to the large molecular weight of the colloidal gold-labeled methamphetamine antibody, the SPR response value changes greatly after being combined with the methamphetamine-BSA probe on the surface of the SPR sensor biochip. If the hair sample contains methamphetamine, it binds to the colloidal gold-labeled methamphetamine antibody in the buffer, resulting in a small amount of antibody bound to the methamphetamine-BSA probe on the chip surface, and small changes in the refractive index of the chip medium and SPR resonance peaks , the SPR response value decreased. Therefore, the concentration of methamphetamine molecules in hair samples is inversely proportional to the change in SPR response.

[0077] The methamphetamine channel test results show that the methyl SPR response value of the 200-fold diluted methamphetamine gold-labeled antibody is 24, and the 200-fold diluted methamphetamine gold-label...

Embodiment 3

[0078] Example 3 Hair sample ketamine (KET) detection:

[0079] Due to the large molecular weight of the colloidal gold-labeled ketamine antibody, after it is combined with the ketamine-BSA probe on the surface of the SPR sensor biochip, the SPR response value caused by it changes greatly. If the hair sample contains ketamine, it binds to the colloidal gold-labeled ketamine antibody in the buffer, resulting in a small amount of antibody bound to the ketamine-BSA probe on the chip surface, small changes in the chip medium refractive index and SPR resonance peaks, and a low decrease in the SPR response value . Therefore, the molecular concentration of ketamine in the hair sample is inversely proportional to the change of the SPR response value.

[0080] The test results of the ketamine channel showed that the methyl SPR response value of the 200-fold diluted ketamine gold-labeled antibody was 24, and the 200-fold diluted ketamine gold-labeled antibody and the hand-held colloida...

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PUM

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Abstract

The invention discloses a rapid detection method for drugs in hair, which comprises the following steps: 1) collection of hair drugs; 2) enzymatic extraction of hair drugs; 3) colloidal gold detection of hair samples; 4) SPR of hair samples Detection: The beneficial effect of the present invention is that the rapid extraction of drug molecules from the hair sample can be completed within 1 minute, and the intelligent identification of the hand-held colloidal gold optical module and the automatic detector can complete the low-concentration drug detection in the hair sample digestion solution within 1 minute. For the detection of molecules, the portable plasma resonance (SPR) instrument can complete the detection of trace concentrations of drug molecules in the digestion solution of hair samples within 2 minutes. This method is simple to operate, short in detection time, and low in cost.

Description

technical field [0001] The invention relates to the technical field of biological and criminal identification. In particular, the invention relates to a rapid detection method for drugs in hair. Background technique [0002] Drug abuse is a major problem that seriously endangers social security and human life and health. The number of drug addicts is increasing and younger, and the types of third-generation new drugs such as methcathinone are increasing. At present, immunocolloidal gold technology, gas chromatography-mass spectrometry and high performance liquid chromatography-mass spectrometry are mainly used to detect and identify the urine and saliva of drug addicts at home and abroad. However, usually after 24 to 48 hours of drug use, the drug is basically metabolized and excreted in urine and saliva, resulting in a negative test and unable to provide worthless information. Therefore, at this stage, it is urgent to establish a detection method that is fast, stable in r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/552
CPCG01N21/554
Inventor 陈喆叶肖俊程向荣范春雷朱勤锋
Owner 浙江诺迦生物科技有限公司