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Method for in vitro induction of Pinglu lily polyploids

A technique of polyploidy and in vitro application in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., which can solve problems such as complicated steps, and achieve the effect of cheap reagents, simple operation, and suitable for cultivation

Inactive Publication Date: 2018-11-23
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese patent 201610472915.1 discloses a method for inducing tetraploids of Lilium chinensis. The scales are used as materials, and colchicine is used to induce tetraploids. The steps of regenerating bulbs after the scales are induced are relatively complicated.

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  • Method for in vitro induction of Pinglu lily polyploids
  • Method for in vitro induction of Pinglu lily polyploids
  • Method for in vitro induction of Pinglu lily polyploids

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Embodiment 1

[0027] A method for in vitro inducing polyploidy of Lilium chinensis, comprising the following steps:

[0028] 1) Pre-cultivation of lily bulbs: use the detoxified test tube bulbs of Lily pinnata as explants, inoculate in MS+0.8mg·L -1 6-BA+0.1mg·L -1 NAA+30g·L -1 Sucrose+6g·L -1 Pre-cultivate in agar medium for 40 days, and use the robust and uniform-sized bulbs obtained from the culture as materials for inducing polyploidy. The culture conditions are temperature 24°C, photoperiod 14h·d -1 , the light intensity is 2000Lx;

[0029] 2) Preparation of induction medium: medium MS + 0.8mg·L -1 6-BA+0.1mg·L -1 NAA+30g·L -1 Sucrose+6g·L -1 Trifluralin was added to the agar to a concentration of 200 μmo L -1 , the trifluralin is emulsifiable concentrate 480g L -1 type, autoclaved for use;

[0030] 3) Induction of polyploidy: Peel off the outer layer of scales from the uniform bulbs obtained by pre-cultivation and inoculate them in induction medium MS + 0.8mg·L -1 6-BA+...

Embodiment 2

[0035] A method for in vitro inducing polyploidy of Lilium chinensis, comprising the following steps:

[0036] 1) Pre-cultivation of lily bulbs: use the detoxified test tube bulbs of Lily pinnata as explants, inoculate in MS+0.8mg·L -1 6-BA+0.1mg·L -1 NAA+30g·L -1 Sucrose+6g·L -1 Pre-cultivate in agar medium for 40 days, and use the robust and uniform-sized bulbs obtained from the culture as materials for inducing polyploidy. The culture conditions are temperature 26°C, photoperiod 14h·d -1 , the light intensity is 3000Lx;

[0037] 2) Preparation of induction medium: Second medium MS + 0.8mg·L -1 6-BA+0.1mg·L -1 NAA+30g·L -1 Sucrose+6g·L -1Pendimethalin was added to the agar to a concentration of 200 μmo L -1 , the pendimethalin is emulsifiable concentrate 330g L -1 type, autoclaved for use;

[0038] 3) Induction of polyploidy: Peel off the outer layer of scales from the uniform bulbs obtained by pre-cultivation and inoculate them in induction medium MS + 0.8mg·L ...

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Abstract

The invention relates to a method for in vitro induction of Pinglu lily polyploids. The method comprises the steps of lily bulb pre-culture, induction culture medium preparation, multiploidy induction, induced bulb culture, induced bulb rooting and ploidy determination. According to the method, the Pinglu edible lily detoxication test tube bulbs are used as materials to be subjected to induction treatment by induction agents of pendimethalin or trifluralin so as to obtain tetraploid lilies. The polyploid lilies obtained through chromosome reduplication have the characteristics of fat and thickplant scales, strong bodies, high yield, high quality and the like. The induction agents of pendimethalin or trifluralin are used by the method; the tetraploid lilies are obtained; the quality and the yield of the lilies can be improved; the nutrition value and the economic benefits are further improved.

Description

technical field [0001] The invention relates to the induction of polyploid of edible lily, in particular to a method for inducing polyploid of Lilium chinensis. Background technique [0002] Shanxi Pinglu Lily has a long history of cultivation, good quality and high nutritional value. It is famous for its large size, thick petals, no moss in the aftertaste, and no firewood in the petals. It has the reputation of "Zhongtiao Ginseng". Pinglu lily contains a lot of protein, fat, starch, vitamins, and a small amount of calcium, phosphorus, iron, etc. It has high medicinal value, has the effects of nourishing the middle and replenishing qi, warming the lungs and relieving cough, calming the nerves and clearing the heart, and has high edible value and commercial value. Long-term asexual reproduction leads to low reproductive rate, virus accumulation, and species degeneration of Lily Pinnata, making it difficult to meet the needs of the market. [0003] Polyploid breeding is an i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/001A01H4/008
Inventor 赵娟尹艺臻尹美强张彬温银元宋喜娥董淑琦原向阳
Owner SHANXI AGRI UNIV