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Ternary compound biological fungicide and application to prevention of ginger blast

A biological inoculum and a technology for ginger blast, which is applied to a ternary compound biological inoculum and its application in the prevention and treatment of ginger blast, so as to improve people's living standards and quality of life, reduce the occurrence of ginger blast, and significantly prevent and control the disease. The effect of the control effect

Active Publication Date: 2018-11-23
唐山市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports on the screening of efficient, economical, green, organic and safe green ginger blast control biological agents, and the whole process of biological control of ginger blast

Method used

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  • Ternary compound biological fungicide and application to prevention of ginger blast
  • Ternary compound biological fungicide and application to prevention of ginger blast
  • Ternary compound biological fungicide and application to prevention of ginger blast

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, biomedicine screening-indoor toxicity assay

[0037] 1.1 Single dose comparison

[0038] Select thiazole zinc (40% thiazole zinc SC), Chunlei · Wang copper (47% Chunlei · Wang copper WP), root huoling (70% disulfone sodium WP), copper hydroxide (46% copper hydroxide WG) 4 kinds Chemical agents and Bacillus amyloliquefaciens (effective viable count ≥ 120 million / gWP), Zhongshengmycin (6% Zhongshengmycin WP), Pseudomonas fluorescens (effective viable count ≥ 500 million / gWP), Five kinds of biopharmaceuticals, Neophytomycin (50% Neophytomycin WP) and Bacillus subtilis (effective number of viable bacteria ≥ 1 billion / g WP), were tested for indoor toxicity by the zone of inhibition method, and the effects of the nine kinds of agents were compared. Bacteriostasis effect, screening single agent and compound agent with better effect for field test.

[0039] Cultivate the test strain (causative bacterium of ginger blast) on the NA slant medium for 24 hours, and m...

Embodiment 2

[0050] Embodiment 2, biological control test

[0051] In order to achieve the green control effect of ginger blast, the method of biological control was selected for the control of ginger blast. Taking Tangshan ginger as the test material, thiazole zinc, Chunlei · Wang copper, copper hydroxide, Zhongshengmycin, and new phytomycin with antibacterial effects were selected as single agent and binary compound biological agent (during use, the solvent is water, and the effective viable count concentrations of Pseudomonas fluorescens and Bacillus subtilis are respectively 4.5×10 9 CFU / L, 3.5×10 9 CFU / L) and ternary compound biological agent (when used, the solvent is water, the ratio of effective viable bacteria of Bacillus amyloliquefaciens, Pseudomonas fluorescens and Bacillus subtilis is: 1:10.4:8.3, concentration Respectively: 0.4×10 9 CFU / L, 4.17×10 9 CFU / L, 3.33×10 9 CFU / L), follow-up seed soaking and field ginger blast biological control and control experiments were carr...

Embodiment 3

[0089] Carry out ternary compound biological bacterial agent (when using, solvent is Water, the ratio of effective viable bacteria of Bacillus amyloliquefaciens, Pseudomonas fluorescens and Bacillus subtilis is: 1:10.4:8.3, the concentration is respectively: 0.4×10 9 CFU / L, 4.17×10 9 CFU / L, 3.33×10 9 CFU / L) demonstration of green control technology for ginger blast prevention and control, with a demonstration area of ​​6667m 2 (10 mu), planting density 5000 plants / 667m 2 . See Table 8 for comprehensive green control techniques and control methods. Investigate the occurrence of ginger blast during the period of large-scale soil cultivation of ginger, and randomly select 3 points in the disease center of the demonstration area and the control area, each point is 667m 2 , count the number of ginger blast diseased plants, and calculate the disease index and control effect. The grading standard of ginger blast is carried out according to Table 8. Yields were calculated after...

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Abstract

The invention relates to a ternary compound biological fungicide and an application to prevention of ginger blast. The ternary compound biological fungicide comprises bacillus amyloliquefaciens, pseudomonas fluorescens and bacillus subtilis, wherein a ratio of living bacteria count is 1: (9 to 11): (7 to 10), the dosage form of the ternary compound biological fungicide can be liquid, the living bacteria count of the bacillus amyloliquefaciens is 0.1*10<9>CFU / L-0.9*10<9>CFU / L and preferably 0.4*10<9>CFU / L; the living bacteria count of the pseudomonas fluorescens is 2.09*10<9>CFU / L-9.05*10<9>CFU / L and preferably 4.17*10<9>CFU / L; and the living bacteria count of the bacillus subtilis is 1.65*10<9>CFU / L to 6.75*10<9>CFU / L and preferably 3.33*10<9>CFU / L. The application period of the ternary compound biological fungicide for preventing the ginger blast comprises a seed soaking period, a seedling emergence period, a three-bifurcation period, a small soil dressing period, a large soil dressing period, or a ginger blast occurrence period. The ternary compound biological fungicide has practical significance for the continuous development of the modern agriculture and the food safety, and for improving the living standard and living quality of people.

Description

technical field [0001] The invention relates to the field of biological agents, in particular to a ternary compound biological agent and its application in the prevention and treatment of ginger blast. Background technique [0002] Ginger, also known as ginger, Zingiberaceae (Zingiber offcinale Ross.), is a perennial herbaceous plant, usually fresh rhizomes are harvested, and the rhizomes have a special spicy flavor. They are not only important condiments and Chinese medicinal materials, but also It is an important industrial raw material, which can be processed into a variety of foods and spices. It is an important edible condiment and traditional Chinese medicinal material in our country. Ginger is a vegetable crop that has been planted for a long time and eaten in large quantities around the world. China is one of the main ginger-growing countries in the world, accounting for about 48% of the world's ginger cultivation area. In recent years, my country's ginger industry h...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P1/00A01G22/25A01C1/00A01G7/06A01G13/00
CPCA01C1/00A01G7/06A01G13/00A01N63/00A01G22/25
Inventor 王向东王永存付丽军马艳芝张胜珍客绍英
Owner 唐山市农业科学研究院