Method for continuously extracting cynarin and chlorogenic acid from artichoke leaves and application thereof

Abstract

The invention discloses a method for continuously extracting cynarin and chlorogenic acid from artichoke leaves, which comprises the following step: S1, selecting artichoke leaves without mildew, spots and pest and disease damage, then drying the artichoke leaves for 1 to 2h at a temperature of 35 to 45 DEG C, and then carrying out grinding. According to the method for continuously extracting thecynarin and the chlorogenic acid from the artichoke leaves and application thereof, which are disclosed by the invention, in the cynarin and chlorogenic acid extraction method, firstly, ethyl alcoholis added, then ultrasonic extraction is carried out to extract the chlorogenic acid, and then the cynarin is extracted by heating reflux; the method ingeniously and reasonably controls the temperature, firstly, in the chlorogenic acid extraction, the temperature is controlled within a range of 20 to 60 DEG C so as to prevent damage to the chlorogenic acid, and in the cynarin extraction, a high temperature is adopted so as to improve an extraction rate; moreover, a response surface is adopted to carry out optimization so as to improve the extraction rate of the cynarin and the chlorogenic acid.

Description

technical field [0001] The invention relates to the technical field of artichoke leaf extraction, in particular to a method for continuously extracting cynarin and chlorogenic acid from artichoke leaves and an application thereof. Background technique [0002] The leaves of artichoke are rich in artichoke acid, chlorogenic acid, etc. Artichoke acid, also known as artichoke, exists in artichoke, forest Senecio whole grass, and can also be chemically synthesized. It has the effect of treating chronic hepatitis and lowering cholesterol, and can benefit the liver and protect the gallbladder. Chlorogenic acid is a depsipolic acid produced by caffeic acid and quinic acid. It is a kind of shikimic acid produced by plants during aerobic respiration. Phenylpropanoid compounds have various pharmacological functions such as antibacterial, antiviral, anti-inflammatory, antipyretic, and antiallergic, and can be used for food preservation to effectively prevent spoilage of beverages and f...

AI Technical Summary

Problems solved by technology

[0003] In the existing literature, cynarin and chlorogenic acid are mostly extracted separately, and cynarin and chlorogenic acid are rarely extracted continuously from artichoke leaves. Chinese patent literature (publication number: CN107854506A) discloses a kind of artichoke The preparation process of the extract and its detection method, the preparation process includes the following steps: adding artichoke into the reaction kettle, and then adding water; heating to the temperature in the reaction kettle is 60 ° C, then opening the condenser to circulate the condensed water, when the temperature rises to Turn on heat reflux at 90-95°C; repeat step 2 twice; filter the substance obtained in step 3 through the first filter membrane to obtain the extract, and concentrate the concentration of the extract to 1:1-1:2mL / g or relative density to 1.08-1.15, use ethanol solutions of different concentrations to ethanol precipitation to obtain the supernatant, and filter the supernatant through the second filter membrane to obtain the filtrate; absorb the filtrate through the resin column; then wash with water and alcohol to wash with alcohol After the liquid is filtered through a microporous filter to remove impurities, it is concentrated in a vacuum to obtain a thick slurry, which is then spray-dried and sieved to obtain an artichoke extract; , chlorogenic acid will be destroyed, in addition, the preparation process has not been optimized, the extraction efficiency is not very good, still needs further improvement

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