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A method for isolating inosine from sea squirt

A technology of ascidian and inosine, which is applied in the field of compound inosine, can solve the problems of obtaining inosine separation technology reports and no research revealing ascidian inosine, and achieves separation steps that are simple and easy to operate, without loss of samples, Effect of Rapid Separation Amount

Active Publication Date: 2020-06-26
XIAMEN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there is no research to reveal whether inosine is contained in Ascidian ascidian, and there is no report on how to obtain inosine from Ascidian ascidian

Method used

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  • A method for isolating inosine from sea squirt
  • A method for isolating inosine from sea squirt
  • A method for isolating inosine from sea squirt

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Embodiment Construction

[0030] Below by embodiment the content of the present invention is specified:

[0031] 1) Freeze-dry ascidian with a wet weight of 10Kg, cut it into pieces, crush it with a pulverizer, put it in a 5000mL beaker, soak it in n-butanol and extract it with ultrasonic acceleration, repeat the extraction several times until the extract becomes light After being nearly colorless, the extracts were combined, and the solvent was dried by a rotary evaporator to obtain 52 g of the crude extract (qb) of the n-butanol layer;

[0032] 2) Take 52 g of the n-butanol layer crude extract (qb) obtained in step 1) and dissolve it in 75 mL of 50% methanol water to make the concentration 693 mg / mL, and separate it by high-speed countercurrent liquid chromatography (HSCCC) (TBE-300B) Cut into 4 components; divided into five loading times, each loading volume is 15mL;

[0033] High-speed countercurrent liquid chromatography system TBE-300B; the solvent system is ethyl acetate: methanol: water = 5:1:...

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Abstract

The invention discloses a method for separating inosine from styela plicata. According to the method, a compound inosine is extracted, separated and identified from the styela plicata for the first time, the styela plicata can be sufficiently recycled and utilized and wastes are avoided; high-speed counter-current chromatography is used for carrying out separation so that the method has the advantages of no sample loss, no pollution, high efficiency, rapid speed and great separation amount; specific separation steps are simple and easy to operate; the separated inosine has wide application inthe fields of pharmaceuticals and the like and has very important meanings on researches of effective components of ocean natural products.

Description

technical field [0001] The invention relates to the technical field of extraction and separation technology for new drugs from biological sources, in particular to a method for separating inosine from Styela plicata, and the compound inosine is extracted, separated and identified from Styela plicata for the first time. Background technique [0002] There are more than 1500 species known in nature, mainly distributed in tropical and subtropical seas. Our country is rich in sea squirt resources. There are more than 100 species of sea squirts recorded in China's coastal areas, and many of them are unique to my country. Generally speaking, sea squirts can be wild type (wild type) collected from coral reef waters, or cultured type (cultured type) obtained through cultivation. According to reports, sea squirts contain a variety of compounds with anti-tumor, anti-virus, anti-bacterial, anti-inflammatory and other activities. Therefore, in recent years, the chemical composition and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/167C07H1/08A61P7/00A61P9/00A61P1/16A61P27/02A61P25/00
CPCA61P1/16A61P7/00A61P9/00A61P25/00A61P27/02C07H1/08C07H19/167
Inventor 陈永轩吴秀娜李培铖罗联忠许莉秦飞陈丹
Owner XIAMEN MEDICAL COLLEGE