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Strain of agaricus bisporus and breeding method thereof

A technology of Agaricus bisporus and strains, applied in the field of bioengineering, can solve problems such as changing the genetic characteristics of species, and achieve the effects of dense mycelium growth, fast plate growth rate, and improved substrate utilization.

Inactive Publication Date: 2018-11-23
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

[0014] Genome rearrangement breeding technology is a milestone in changing the genetic characteristics of species. It combines the advantages of traditional breeding methods and protoplast fusion technology. At present, there is no application of genome rearrangement technology to breed Agaricus bisporus strains at home and abroad. to report

Method used

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  • Strain of agaricus bisporus and breeding method thereof
  • Strain of agaricus bisporus and breeding method thereof
  • Strain of agaricus bisporus and breeding method thereof

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Embodiment

[0058] The breeding method of embodiment Agaricus bisporus bacterial strain comprises the steps:

[0059] 1. Protoplast Preparation

[0060] Inoculate the starting strain Zongxiu No. 1 on the potato dextrose agar (PDA) solid medium, culture at a constant temperature of 25±0.5°C for 10 days, take the vigorously growing mycelium on the PDA solid medium, and inoculate it into a conical flask filled with 100mL of PD medium medium, 25°C, shake once every 12h, culture for 7 days, collect the mycelium, beat it with a sterile homogenizer to crush it, absorb 1mL of bacterial liquid into 100mL liquid potato glucose straw soaking juice medium, and cultivate for 10d at 25°C .

[0061] Take the brown Agaricus bisporus mycelium that has been cultured for 10 days, filter it with four layers of sterilized gauze, then rinse it with sterile water for 3 times, dry it with sterile paper, and suspend it in an appropriate amount of bisporus that has been prepared at a certain concentration. In so...

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Abstract

The invention relates to a strain of agaricus bisporus and a breeding method thereof. The strain of agaricus bisporus has preservation number of CCTCC NO: M2017244. The strain has colony fully growingon a flat plate for 9d, the flat plate mycelium is white, the formed primordium number is 151-202 / m<2>, mature fruiting body pileusis is brown, and the per unit area yield of factory cultivation is 26kg / m<2> or above. The strain of agricus bisporus is bred by adopting a genome rearrangement technology, and has the advantages of high flat plate growth rate, dense mycelium growth, obviously increased induction number of fruiting body primordium, improved yield of agaricus bisporus, high matrix utilization rate, easy factory cultivation, and increased economic benefits.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Agaricus bisporus strain and a breeding method thereof. Background technique [0002] Agaricus bisporus (Lange) sing. Taxonomically belongs to Eumycota, Basidiomycetetes, Agaricales, and Agaricus in the family Agaricaceae. [0003] The main characteristics of the mushroom genus are the free gills, the cap and the stipe are easy to separate, there are bacterial rings, and the spore print is purple-brown. The reproduction of Agaricus bisporus has two ways: asexual reproduction and sexual reproduction, among which sexual reproduction is the main part of the life cycle of Agaricus bisporus. [0004] In the life history of Agaricus bisporus, there are mainly special and complex secondary conjoint genetic patterns. In 1972, Raper et al. described the life history of Agaricus bisporus in detail. In Agaricus bisporus, the bisporus basidium accounts for the vast ma...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01H15/00C12N15/04C12Q1/6895C12R1/645
CPCA01H15/00C12N15/04C12Q1/6895C12N1/145C12R2001/645
Inventor 王金斌唐雪明李文李正鹏蒋玮
Owner SHANGHAI ACAD OF AGRI SCI
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