Nonreactive fermented concentrated feed for lactating sows and application thereof

A technology for sucking sows and concentrated feed, which is applied in applications, animal feed, animal feed, etc., can solve the problems of insufficient nutritional supply for suckling sows and lack of regulation of bacterial flora in sows, so as to improve production capacity and piglet survival. rate and growth rate, promote rapid recovery, reduce labor stress

Inactive Publication Date: 2018-11-27
HUNAN LIFENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides an antibiotic-free fermented concentrated feed for lactating sows and its application, so as to solve the problem that the nutritional supply of conventional feeds to lactating sows is not comprehensive enough in the prior art.
[0005] Another technical problem to be solved by the present invention is that the conventional feed in the prior art lacks the regulating effect on the flora in the sow

Method used

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  • Nonreactive fermented concentrated feed for lactating sows and application thereof
  • Nonreactive fermented concentrated feed for lactating sows and application thereof
  • Nonreactive fermented concentrated feed for lactating sows and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Isolation and screening of specific microorganisms: The heart of healthy weaned piglets was bled, aseptically operated, and the digestive tract was isolated. The contents of duodenum, jejunum, ileum, cecum, colon, and rectum were collected respectively, placed in 5ml sterilized normal saline and mixed for 10 minutes, and the supernatant was taken.

[0032] A. Isolation of lactic acid bacteria: spread the supernatant on MC solid medium, incubate at a constant temperature of 37°C for 24 hours, pick colonies with calcium-dissolving circles, and repeatedly streak and separate until pure strains are obtained.

[0033] B. Isolation of Bacillus: inoculate the supernatant in nutrient broth, culture at 37°C, 200r / min for 24h. Take the culture solution and place it in a water bath at 80°C for 15 minutes to kill non-spore-forming bacteria, inoculate it in nutrient broth, and culture it for enrichment at 37°C and 200 r / min for 20 hours. The bacterial solution after enrichment cult...

Embodiment 2

[0036] Preparation of bacterial strain A (lactic acid bacteria) fermentation broth:

[0037] Take 2mL strain A (viable bacteria concentration is 10 10 CFU / mL), inoculated in 100mL medium for shake flask fermentation culture, the fermentation temperature is 28-37°C, the pH value is 6.0-7.0, the rotation speed is 200-300r / min, and the fermentation time is 24-48h. Shake flask fermentation medium is MRS medium, the ingredients are: beef protein powder 10g, fish juice 10g, yeast extract juice powder 5g, glucose 20g, sodium acetate 5g, diammonium citrate 2g, magnesium sulfate 0.58g, manganese sulfate 0.28g, Add 15 g of agar powder, add 1 g of Tween 80, dissolve in 1000 mL of distilled water, and adjust the pH value to 6.0-7.0.

[0038] After the shake flask fermentation, carry out the expansion culture of the fermenter, take 100mL shake flask fermentation seed liquid and inoculate it into a 10L fermenter, the liquid volume is 5L, the fermentation temperature is 30~37℃, the pH value...

Embodiment 3

[0040] Preparation of strain B (Bacillus) fermentation broth:

[0041] Take 2mL strain B (the concentration of live bacteria is 10 10 CFU / mL), inoculated in 100mL medium for shake flask fermentation culture, the fermentation temperature is 28-37°C, the pH value is 7.2, the rotation speed is 200-300r / min, and the fermentation time is 24-36h. The shake flask fermentation medium is LB medium, the ingredients are: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH value is 7.2.

[0042] After the shake flask fermentation is completed, carry out the expansion culture of the fermenter. Take 100mL shake flask fermentation seed liquid and inoculate it into a 10L fermenter. The liquid volume is 5L. min, ferment for 24-36 hours. The composition of the medium in the pilot test of the fermenter was the same as that of the shake flask fermentation medium. After the fermentation, the medium was kept at 4°C for later use.

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Abstract

The invention provides nonreactive fermented concentrated feed for lactating sows and application thereof and belongs to the technical field of feed and fermentation. The nonreactive fermented concentrated feed is prepared by screening bacillus subtilis, saccharomycetes and lactobacillus from piglet intestinal tract content, respectively fermenting the three strains to prepare seed liquid, respectively adjusting thalli concentration, blending in an isovolumetric manner, mixing with 12.5% lactating sow concentrated feed not containing antibiotics and chemical drug, bagging and sealing for fermentation. The prepared feed has clear adjusting effect on pig gastrointestinal tract functions and can improve nutritional value of the feed. By preparing the prepared feed into complete feed by a proportion of 20% for sow feeding, constipation of the sows can be reduced, feed intake of the lactating sows is increased, milk yield is increased, and weaned piglet weight is increased, so that capacityof the sows and survival rate and growth speed of piglets are increased; by applying the feed, antibiotic consumption can be reduced correspondingly, generation of drug-tolerant strains is relieved,ammonium odor is improved, and the feed is of positive significance for ecological environment.

Description

technical field [0001] The invention relates to the technical field of animal feed, further relates to the preparation and application of fermented feed, in particular to an antibiotic-free fermented concentrated feed for lactating sows and its application. Background technique [0002] In the process of modern pig production, a series of sow reproductive disorders are becoming more and more serious, especially in pig farms where antibiotics are often used, many sows are in a pathological sub-health state, resulting in a decline in production performance. Due to the frequent use of antibiotics and chemical preparations, probiotics are also killed while pathogenic bacteria are killed in the intestinal tract, which destroys the micro-ecological balance in the intestinal tract, resulting in imbalanced growth of beneficial bacteria, constipation, metritis, mastitis, etc. Diseases increase, and at the same time, piglets will have multiple intestinal diseases and high mortality, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K10/12A23K10/30A23K10/22A23K20/158A23K20/142A23K20/105A23K20/22A23K20/24A23K20/20A23K20/28A23K20/26A23K20/189A23K20/174A23K10/37C12N1/20C12N1/16C12N1/02
CPCA23K10/12A23K10/22A23K10/30A23K10/37A23K20/105A23K20/142A23K20/158A23K20/174A23K20/189A23K20/20A23K20/22A23K20/24A23K20/26A23K20/28A23K20/30A23K50/30C12N1/02C12N1/16C12N1/20Y02P60/87
Inventor 陈晓安王刚
Owner HUNAN LIFENG BIOTECH CO LTD
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