LED array based microchannel rotation cell phase contrast imaging method and system

Abstract

The invention discloses an LED array based microchannel rotation cell phase contrast imaging method and system. The system sequentially comprises, along the light path transmission direction, an LED array, a microchannel, a microscope, a Cmos camera acquisition module, a rotation cell feature extraction and recognition module, a cell rotation measurement module, a cell blur removal module, an image cell rotation correction module and a rotation cell phase contrast imaging module. Phase contrast imaging is the most effective method for imaging transparent samples and can obtain details of contours of the sample that are invisible on normal intensity imaging; with the method, blurring caused by rapid movement of cells within the one exposure time in the microchannel can be removed. In the method, LED is used as an asymmetric light source to analyze and study phase contrast imaging of the rotation cell imaging in the microchannel, and in the case of asymmetric illumination, cells in all forms of movement in the microchannel can be imaged perfectly to assist as in morphological analysis and learning.

Description

technical field [0001] The invention belongs to the technical field of microscopic phase-contrast imaging, and in particular relates to a method and system for phase-contrast imaging of rotating cells in a microfluidic channel based on an LED array. Background technique [0002] Recent impressive growth has occurred in the state-of-the-art of laboratory-on-a-chip (LOC) devices, which allow the realization of compact structures at the microscopic scale, enabling the processing of samples such as reagents, microcells, etc. In this structure, optical microscopy applied to microfluidic devices has become an increasing concern for real-time observation of biochemical processes in microfluidic channels, counting, velocity measurement, etc. [0003] In biology and medicine, there are significant imaging in biological cell sample morphology analysis, structure, identification, etc., and in the analysis of biological samples to miniaturization, detail, living body, movement and the a...

AI Technical Summary

Problems solved by technology

[0004] In the microfluidic channel, most of the suspended sample cells have basically no rotation and movement under high response exposure time, but a small number of cells move too fast and rotate, resulting in the final phase contrast imaging, the rotating cells cannot be accurately carried out. Matching and Correction

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