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Fluorescent immunochromatographic test strip and reagent card

A technology of fluorescent immunochromatography and test strips, which is applied in the field of immunoassay, can solve the problems of low detection efficiency and accuracy, low detection sensitivity, etc., and achieve high detection efficiency and accuracy, high detection sensitivity, and extensive industrial value Effect

Inactive Publication Date: 2018-11-30
德康润生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a fluorescent immunochromatographic test strip to solve the technical problems of low detection sensitivity and low detection efficiency and accuracy in the prior art
[0007] The object of the present invention is also to provide a reagent card to solve the technical problems of low detection sensitivity and low detection efficiency and accuracy in the prior art

Method used

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  • Fluorescent immunochromatographic test strip and reagent card
  • Fluorescent immunochromatographic test strip and reagent card
  • Fluorescent immunochromatographic test strip and reagent card

Examples

Experimental program
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Embodiment 1

[0040] See figure 1 As shown, this embodiment provides a fluorescence immunochromatographic test strip, which includes a bottom plate 101 on which a sample pad 102, a fluorescent pad 103, a nitrocellulose membrane 104, and a sample suction pad are sequentially arranged along its length. 105; The sample pad 102 partially overlaps the fluorescent pad 103, the fluorescent pad 103 partially overlaps the nitrocellulose film 104, and one side of the fluorescent pad 103 is located below the sample pad 102, and the opposite side of the fluorescent pad 103 is located Above one side of the nitrocellulose membrane 104; the nitrocellulose membrane 104 partially overlaps the sample suction pad 105, and the opposite side of the nitrocellulose membrane 104 is located below the sample suction pad 105; nitrocellulose membrane 104 is provided with a detection line 106 and a quality control line 107, and the detection line 106 is close to the fluorescent pad 103; the sample pad 102 is treated with...

experiment example 1

[0061] In order to further illustrate the beneficial effects of the fluorescence immunochromatographic test strips provided by the present invention, the fluorescence immunochromatographic test strips provided in Example 1 and the fluorescence immunochromatographic test strips provided in the comparative example are used for different concentrations of S100-β protein samples were measured, where the fluorescent immunochromatographic test strip provided in this example was used for the experimental group, and the fluorescent immunochromatographic test strip provided by the comparative example was used for the control group.

[0062] The concentration of the S100-β protein sample and the corresponding detection line signal value and quality control line signal value are shown in Table 1. Among them, the detection line signal value and quality control line signal on the fluorescence immunochromatographic test strip provided by the comparative example The values ​​are represented by T...

experiment example 2

[0079] The difference from experimental example 1 is that the measured samples and sample concentrations are different. The sample used in this experimental example is N-terminal pro-brain natriuretic peptide (NT-proBNP), and the detection line signal value and quality control line corresponding to different sample concentrations The signal value is shown in Table 4.

[0080] Table 4 Detection line signal value and quality control line signal value corresponding to different sample concentrations

[0081]

[0082]

[0083] Perform homogeneity analysis on the data in Table 4, and calculate the coefficient of variation (CV) of the three sets of T1 / C1 values ​​and T2 / C2 values ​​corresponding to the concentration of each sample. The results are shown in Table 5 and can be seen from Table 5. The CV value of the experimental group and the control group are both below 10%, and the CV value of the experimental group is lower than that of the control group. Therefore, adding bromocresol gr...

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Abstract

The invention relates to the technical field of immunodetection, and in particular relates to a fluorescent immunochromatographic test strip and a reagent card. The fluorescent immunochromatographic test strip comprises a bottom plate, and a sample pad, a fluorescent pad, a nitrocellulose membrane and a sample absorption pad are sequentially disposed on the bottom plate along the longitudinal direction of the bottom plate; the sample pad and the fluorescent pad partially overlap, the fluorescent pad and the nitrocellulose membrane partially overlap, one side of the fluorescent pad is located below the sample pad, and the other opposite side of the fluorescent pad is located above one side of the nitrocellulose membrane; the nitrocellulose membrane the and aspiration pad partially overlap,and the other opposite side of the nitrocellulose membrane is located below the sample absorption pad; the nitrocellulose membrane is provided with a detection line and a quality control line, and thedetection line is close to the fluorescent pad; the sample pad is treated with a sample pad treatment solution which contains 0.1% to 0.5% of a bromocresol green ethanol solution having a concentration of 10%. The fluorescent immunochromatographic test strip has high detection sensitivity and high detection efficiency and accuracy.

Description

Technical field [0001] The invention relates to the technical field of immunodetection, in particular to a fluorescent immunochromatographic test strip and a reagent card. Background technique [0002] The immunochromatography method is intuitive, fast, easy to operate, and low in cost. The test fluid can be blood, urine, saliva, etc. Therefore, its application in rapid clinical diagnosis, in the field and in places where experimental conditions are not available, is developing rapidly. Colloidal gold immunochromatographic test paper is the most widely used. With the help of the advancement of nitrocellulose membrane manufacturing and the maturity of antibody preparation and other supporting technologies, since the first immunochromatographic kit was introduced to the market in the late 1980s, Immunochromatography technology has been applied in the field of clinical surveillance, including pregnancy detection, infectious disease detection, hepatitis B surface antigen, cardiac tro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/533G01N33/543
CPCG01N33/533G01N33/54306G01N33/558
Inventor 董常慧王昊杨玮刘琦冯建敏张林杰
Owner 德康润生物科技(北京)有限公司
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