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Immunofluorescence kit for rapid quantitative detection of cardiac troponin I, creatine kinase isoenzyme and myoglobin

A technique for cardiac troponin and creatine kinase, which is applied in the biological field and can solve the problems of short detection time and lack of accuracy.

Inactive Publication Date: 2018-11-30
RELIA BIOTECH JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is a lack of kits for cardiac troponin I, creatine kinase isozyme and myoglobin with high accuracy, high sensitivity and short detection time

Method used

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  • Immunofluorescence kit for rapid quantitative detection of cardiac troponin I, creatine kinase isoenzyme and myoglobin
  • Immunofluorescence kit for rapid quantitative detection of cardiac troponin I, creatine kinase isoenzyme and myoglobin
  • Immunofluorescence kit for rapid quantitative detection of cardiac troponin I, creatine kinase isoenzyme and myoglobin

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Example 1 Preparation of Immunofluorescence Reagent Strips for Quantitative Detection of Cardiac Troponin I, Creatine Kinase Isoenzyme and Myoglobin

[0019] Such as figure 1 , an immunofluorescence test strip for quantitatively detecting cardiac troponin I, creatine kinase isoenzyme and myoglobin, the test strip includes a support pad 5, a detection membrane 4 arranged on the support pad, the detection membrane The two ends are respectively connected with a filter gasket 3 and a water-absorbing gasket 6, the filter gasket is connected with a conjugate gasket 2, and the other end of the conjugate gasket is connected with a sample gasket 1, wherein the sample gasket is coated with red blood cells to block There are cardiac troponin I test strips, creatine kinase isoenzyme test strips, myoglobin test strips and quality control strips on the detection membrane from the sample gasket to the water-absorbent gasket in sequence, cardiac troponin I test strips Cardiac troponin...

Embodiment 2

[0029] Embodiment 2 Sensitivity and detection linear range of the kit of the present invention

[0030] The method of using the immunofluorescence test strip (prepared in Example 1) of the present invention: take 60 μl of the plasma sample of the clinical patient and add it dropwise on the sample pad of the immunofluorescence test strip, and after incubating at 18°C ​​for 8min, place it in the Relais The TZ-301 immunofluorescence detector was used for detection, and the cTnI, CK-MB and Myoglobin concentrations were calculated according to conventional methods. Specifically, the relative optical density was calculated by detecting the fluorescence intensity on the test strip and the quality control strip, and then by Relative optical density-concentration standard curves were used to calculate the concentrations of cTnI (cardiac troponin I), CK-MB (creatine kinase isoenzyme) and Myoglobin (myoglobin). The clinical cTnI-positive plasma sample was diluted multiple times, and the ...

Embodiment 3

[0039] The repeatability and precision of embodiment 3 kits of the present invention

[0040] One clinical sample was detected by using the test strip of the present invention (prepared in Example 1), and the detection was repeated 10 times for each sample. Concrete detection method: get clinical patient sample (serum) 60ul and add on the sample pad of the test strip of the present invention, after incubating 8min under 18 ℃ of conditions, be placed in the Rui Lai TZ-301 type immunofluorescence detector and detect, pass Detect the fluorescence intensity on the test strip and the quality control strip, and obtain the concentrations of cTnI, CK-MB, and Myoglobin according to the preset calculation formula and conversion curve, perform mean and variance analysis on the same sample test results, and calculate the coefficient of variation, The results are shown in Table 4.

[0041] Table 4 test strip of the present invention is to the detection result of cTnI, CK-MB and Myoglobin ...

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Abstract

The invention provides an immunofluorescence kit for rapid quantitative detection of cardiac troponin I, creatine kinase isoenzyme and myoglobin, and relates to the field of biotechnology. The kit includes an immunofluorescence test strip which includes a support gasket, a test membrane, a conjugate gasket, a filter gasket, a water absorbent gasket and a sample gasket; the sample gasket is coatedwith an erythrocyte blocker, cardiac troponin I, creatine kinase isoenzyme and myoglobin test bands and a quality control band are arranged on the test membrane, the cardiac troponin I, creatine kinase isoenzyme and myoglobin test bands are respectively coated with cardiac troponin I antibody A, creatine kinase isoenzyme antibody A and myoglobin antibody A, the quality control band is coated withanti-streptavidin antibody, and the conjugate gasket is coated with a heterophilic antibody blocker and a fluorescent dye conjugate. The immunofluorescence kit can simultaneously, fast, accurately andhighly-sensitively detect the cardiac troponin I, the creatine kinase isozyme and the myoglobin in a sample, and is helpful for early and rapid diagnosis of acute myocardial infarction.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an immunofluorescence kit for rapid and quantitative detection of cardiac troponin I, creatine kinase isozyme and myoglobin. Background technique [0002] Acute myocardial infarction (AMI) is myocardial necrosis caused by acute and persistent coronary ischemia and hypoxia. Clinically, there are often severe and persistent substernal pains, which cannot be completely relieved by rest and nitrates, accompanied by increased serum myocardial enzyme activity and progressive ECG changes, which may be complicated by arrhythmia, shock or heart failure, often life-threatening. After acute myocardial infarction, if a clear diagnosis and proper treatment cannot be obtained within a few hours, the myocardium will cause irreversible necrosis due to ischemia and hypoxia; in the diagnosis process of acute myocardial infarction, electrocardiogram is one of the indispensable detection items. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/573G01N33/533G01N33/577G01N33/58
CPCG01N33/533G01N33/573G01N33/577G01N33/582G01N33/6887G01N2800/325
Inventor 陈子健刘建源郁靓朱奇峰宗荣芳钱亦妮李娜於敏高月秀解慧梅郝福星邱树磊郭方超
Owner RELIA BIOTECH JIANGSU