Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for separating phospholipid monomers in phospholipid homologues by polyionic liquid

A technology of polyionic liquid and ionic liquid, applied in the fields of chemical engineering and methods, can solve the problems of low product purity and quality, large investment in disposable equipment, and difficulty in taking into account product purity and yield.

Active Publication Date: 2021-01-08
ZHEJIANG UNIV +1
View PDF15 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patents ZL 02121550.2 and ZL 02149601.3 disclose a method for preparing phosphatidylcholine by multistage countercurrent leaching of soybean powder phospholipids using a mixed solvent of acetonitrile, methanol, ethanol and other monohydric low-carbon alcohols. The content of phosphatidylcholine is about 80%, the product purity and quality obtained by this method are still low, and there is the deficiency that product purity and yield are difficult to balance in countercurrent extraction technology
Supercritical fluid extraction using supercritical CO 2 As an extraction agent, the process is green and polluting, but there is a lack of large investment in disposable equipment. At the same time, the method is usually operated in a kettle, and the product yield is low and the purity is not high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for separating phospholipid monomers in phospholipid homologues by polyionic liquid
  • A method for separating phospholipid monomers in phospholipid homologues by polyionic liquid
  • A method for separating phospholipid monomers in phospholipid homologues by polyionic liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Porous polyionic liquid was prepared by using 1-vinyl-3-octyl imidazolium bromide as monomer. In a 50 mL Schlenk storage bottle, dissolve 1.00 g of divinylbenzene, 0.59 g of 1-vinyl-3-octylimidazolium bromide, and 31.8 mg of azobisisobutyronitrile in 11 mL of ethyl acetate, ethanol, and water ( In a mixed solution with a volume ratio of 8:2:1), the reaction was stirred for 24 hours at 80°C. After cooling at room temperature, it was washed with ethanol, dried under vacuum at 65°C for 24 hours, and ground for use. As determined by a nitrogen adsorption instrument, the product exhibits obvious mesoporous characteristics, with a specific surface area of ​​330m 2 g -1 , the pore volume is 0.30cm 3 g -1 , the pore size is 5.2nm. The ionic liquid content in the porous polyionic liquid structure is calculated from the elemental analysis results, and the ionic liquid content is 0.66mmol / g.

[0063] This embodiment prepares the porous polyionic liquid structural diagram:

...

Embodiment 2

[0068] Porous polyionic liquid was prepared by using 1-vinyl-3-ethylimidazolium octanoate as monomer. In a 50mL Schlenk storage bottle, dissolve 1.00g of divinylbenzene, 1.64g of 1-vinyl-3-ethylimidazolium octanoate and 52.8mg of azobisisobutyronitrile in 20mL of acetonitrile, at 100°C Next, stir the reaction for 24h. After cooling at room temperature, it was washed with ethanol, dried under vacuum at 60°C for 24 hours, and ground for use. As determined by a nitrogen adsorption instrument, the product exhibits obvious mesoporous characteristics, with a specific surface area of ​​352m 2 g -1 , the pore volume is 0.53cm 3 g -1 , the pore size is 7.1nm. The ionic liquid content in the porous polyionic liquid structure is calculated from the elemental analysis results, and the ionic liquid content is 1.02mmol / g.

[0069] The product structural formula that this embodiment prepares is:

[0070]

[0071] Put 20 g of the polyionic liquid prepared above into a chromatographi...

Embodiment 3

[0073]Porous polyionic liquid was prepared by using 1-vinyl-3-butylimidazolium bromide as monomer. In a 50 mL Schlenk storage bottle, dissolve 1.00 g of divinylbenzene, 0.50 g of 1-vinyl-3-butylimidazolium bromide, and 30 mg of azobisisobutyronitrile in 11 mL of ethyl acetate, ethanol, and water (vol. In a mixed solution with a ratio of 8:2:1), the reaction was stirred for 24 hours at 80°C. After cooling at room temperature, it was washed with ethanol, dried under vacuum at 65°C for 24 hours, and ground for use. As determined by a nitrogen adsorption instrument, the product exhibits obvious mesoporous characteristics, with a specific surface area of ​​450m 2 g -1 , the pore volume is 0.38cm 3 g -1 , the pore size is 5.3nm. The ionic liquid content in the porous polyionic liquid structure is calculated from the elemental analysis results, and the ionic liquid content is 0.78mmol / g.

[0074] The product structural formula that this embodiment prepares is:

[0075]

[0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for separating a phospholipid monomer from a phospholipid homolog by a polyionic liquid, and in particular discloses a method for separating the phospholipid homolog to obtain a high-purity phosphatidylcholine monomer. The method uses the porous polyionic liquid as an adsorbent to separate high-purity phosphatidylcholine from a mixed phospholipid crude product. According to the method disclosed by the invention, the polyionic liquid has a specific ionic liquid structure and abundant pore structure, so that the polyionic liquid has the advantages of large adsorption capacity for phospholipid and high monomer selectivity, and can realize selective separation of the phospholipid monomer; under the optimized conditions, the phosphatidylcholine monomer with purity of not less than 98% can be obtained, and the recovery rate is 85% or more; and the method is suitable for industrialized production.

Description

technical field [0001] The invention relates to the field of chemical engineering and technology, in particular to a method for separating phospholipid homologues with polyionic liquids. Background technique [0002] Phospholipids are a class of natural active substances with multiple physiological functions. They are widely present in the cells and tissues of living organisms. Composition of monomers. These phospholipid homologues are similar in structure, but have certain differences in physiological functions and activities. Among them, phosphatidylcholine has the medicinal health effects of preventing fatty liver, lowering cholesterol, and promoting heart health, and is often used as a low-toxic and efficient emulsifier and drug carrier for fat-soluble drug injections, anti-cancer, and sustained-release drugs Medium; Phosphatidylserine has special physiological activity on the brain, and has significant curative effects in repairing brain cells, improving memory, relie...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10
CPCC07F9/103
Inventor 邢华斌师维丁建飞陆建刚任其龙杨启炜张治国赵宁
Owner ZHEJIANG UNIV