dsRNA of wing growth related gene decapentaplegic and application thereof in pest control

A gene and DNA molecular technology, applied in the field of Bactrocera dorsalis control, can solve problems such as heavy workload, impact of import and export trade, difficult and efficient governance, etc., to achieve prevention and control of invasion and spread, good ecological security, good effect on human health

Inactive Publication Date: 2018-12-04
CHINA AGRI UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The insect lays eggs in the fruit epidermis of fruits and vegetables, and the larvae feed on the inside of the fruit, causing the fruit to rot and drop the fruit. Transmission belt, which has a serious impact on import and export trade
At present, measures such as bagging, sexual attraction and application of chemical pesticides are commonly used at home and abroad to control the field damage of Bactrocera dorsalis, but the physical method of bagging is time-consuming and laborious, and chemical pesticides will also bring about "3R" problems, making it difficult to achieve efficient control
In recent years, the male sterile technique of Bactrocera dorsalis (SIT) based on genetic control has opened up a new approach for the control of important economic fruit flies, and has been developed to some extent, but this measure has a large workload and requires continuous release of sterile males

Method used

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  • dsRNA of wing growth related gene decapentaplegic and application thereof in pest control
  • dsRNA of wing growth related gene decapentaplegic and application thereof in pest control
  • dsRNA of wing growth related gene decapentaplegic and application thereof in pest control

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the acquisition of dsRNA of wing development gene decapentaplegic (abbreviated as dpp)

[0051] 1. Samples of Bactrocera dorsalis for testing

[0052] The insect source Bactrocera dorsalis used in this experiment is the indoor population of the Plant Quarantine and Invasion Biology Laboratory of China Agricultural University. The indoor breeding conditions: the temperature is 25°C; the relative humidity is kept at about 70%; D). The breeding method of Bactrocera dorsalis is as follows: Bactrocera dorsalis is reared uniformly in the insect cage, and the larval feed (the formula of the larval feed is shown in Table 1) is added to 500mL distilled water, boiled and bottled for later use, and it is frozen after being cooled. ; Adult feed (adult feed is obtained by mixing sucrose and soybean peptone in a mass ratio of 1:3) is fed to adults after adding water to moisten them. After feeding on the feed, the larvae burrow into the soil to pupate after they are ma...

Embodiment 2

[0157] Embodiment 2, the application of the dsRNA of wing development gene decapentaplegic in the control of Bactrocera dorsalis

[0158] 1. Feeding dsRNA

[0159] The 1st instar healthy and normally developing Bactrocera dorsalis larvae were selected as experimental materials. The experiment was set up with blank control group, dsGFP (irrelevant gene) control group and treatment group. Each group had three replicates, with 40 larvae in each replicate.

[0160] 1. Blank control group (CK)

[0161] Take 20 1.5mL centrifuge tubes, add about 0.15g crushed B. dorsalis larva feed and 4 μL ddHO to each centrifuge tube 2O, mix well. Two larvae were placed in each centrifuge tube for feeding. The feeding and growth of larvae were observed daily. After 48 hours, the feed is completely eaten, and then only supplementary feed is sufficient until the larvae grow to the end of the third instar. One of the surviving larvae in the centrifuge tube was picked into the soil to pupate and e...

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Abstract

The invention discloses dsRNA of a wing growth related gene decapentaplegic and an application thereof in pest control. For the first time, a primer is designed for a citrus fruit fly wing growth genedecapentaplegic, dsRNA is synthesized, the citrus fruit fly decapentaplegic gene is applied to RNAi application by a feeding method, and a wing developmental malformation phenotype is obtained on thepremise that the interference efficiency is guaranteed. The problem that dsRNA injection and introduction are hard to operate in a field is solved, foundation is provided for a method that directly sprays a RNA preparation to control citrus fruit flies to effectively prevent the invasion and diffusion of citrus fruit flies, good economic and social benefits are generated, and the agriculture production and ecological safety of China are protected.

Description

technical field [0001] The invention relates to the fields of biotechnology and agricultural pest control, in particular to the dsRNA of the wing development-related gene decapentaplegic (dpp) and its application in pest control, in particular to its application in the control of Bactrocera dorsalis. Background technique [0002] Bactrocera dorsalis Hendel, also known as oriental fruit fly, belongs to Diptera, Tephritidae, and Bactrocera, and is an important pest of many tropical and subtropical fruits and vegetables. . Bactrocera dorsalis is an omnivorous pest with a wide range of hosts. It can harm more than 250 kinds of fruits and vegetables in more than 40 families such as citrus, guava, carambola, mango, banana, eggplant, pepper, and melon (Deng Zhisheng, 2001; Shang Hongsheng , 1997). The insect lays eggs in the fruit epidermis of fruits and vegetables, and the larvae feed on the inside of the fruit, causing the fruit to rot and drop the fruit. The transmission belt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/11C12N15/87A01K67/033
CPCA01K67/0339A01K2217/05A01K2227/706C12N15/113C12N15/87C12N2310/14
Inventor 李志红郭韶堃沈杰
Owner CHINA AGRI UNIV
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