Shark cartilage glycoprotein and anti-cancer application thereof

A technology of shark cartilage and cartilage sugar, which is applied in the fields of peptide/protein components, ointment delivery, anti-tumor drugs, etc., can solve the problems that have not achieved breakthrough progress, shark cartilage does not have anti-cancer effect, and the treatment effect is not ideal.

Inactive Publication Date: 2018-12-07
车路平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the 1980s, it was discovered that shark cartilage can inhibit angiogenesis, so it was gradually applied to the prevention and treatment of cancer, but the overall therapeutic effect is still not satisfactory
In recent years, people have successively studied the anticancer effect of shark cartilage extract, shark chondroitin, and shark cartilage angiogenesis inhibitory factor, but no breakthrough has been made, and there was even a view that shark cartilage has no anticancer effect

Method used

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  • Shark cartilage glycoprotein and anti-cancer application thereof
  • Shark cartilage glycoprotein and anti-cancer application thereof
  • Shark cartilage glycoprotein and anti-cancer application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: A kind of shark cartilage glycoprotein

[0053] The shark cartilage glycoprotein is prepared according to the following method:

[0054] (1) Shark cartilage 200g, after crushing, pass through a 20-mesh sieve to obtain shark cartilage particles;

[0055] (2) After adding 5 times of pure water to the shark cartilage particles, use NaOH to adjust the pH to 12, heat to 60°C, soak for 5 hours and then filter, the filtrate is set aside, and the filter residue is repeatedly leached twice, and the filtrates are combined;

[0056] (3) After the filtrate was salted out, the free protein was removed by Sevage method, and then 5.6 g of shark cartilage glycoprotein was obtained by lyophilization after dialysis and DEAE-C32 anion interaction resin column chromatography.

Embodiment 2

[0057] Embodiment 2: A kind of anticancer tablet

[0058] Shark cartilage glycoprotein 30g, microcrystalline cellulose 230g, hydroxypropyl cellulose 25g, carboxymethyl starch sodium 15g, magnesium stearate appropriate amount, prepared according to the following method:

[0059] Shark cartilage glycoprotein is crushed through a 60-mesh sieve, added microcrystalline cellulose, made soft with hydroxypropyl cellulose, granulated through a 14-mesh sieve, granulated through a 12-mesh sieve after drying, added with sodium carboxymethyl starch and hard Magnesium fatty acid is mixed, and tabletted to obtain 1000 anticancer tablets.

Embodiment 3

[0060] Embodiment 3: a kind of anticancer external application ointment

[0061] 3 grams of shark cartilage glycoprotein, 30 grams of tortoise shell, 20 grams of abalone shell, 20 grams of baking soda, 20 grams of clam shell, 50 grams of purslane, 100 grams of beef bone meal, 30 grams of Smilax cocos, 80 grams of Arrowhead mushroom, Patrin 30 grams of grass, 10 grams of Houttuynia cordata, 10 grams of astragalus, 10 grams of Qumai, and 10 grams of Lingxiang, prepared according to the following method:

[0062] (1) Grinding tortoise shell, abalone shell, baking soda, clam shell, purslane, beef bone meal, Smilax tuckahoe, Sanzi mushroom, Patrinia, Houttuynia cordata, Astragalus, Qumai, Lingxiang into 180 mesh particles;

[0063] (2) Soak Houttuynia cordata, Linglingxiang, Shanzi mushroom, and Patrinia granules in sea salt for 48 hours. When using, use the water to mix shark cartilage glycoprotein with turtle shell, abalone shell, baking soda, clam shell, Purslane, bovine bone m...

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PUM

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Abstract

The invention relates to a shark cartilage glycoprotein and an anti-cancer application thereof. The shark cartilage glycoprotein is prepared by crushing shark cartilage; extracting the shark cartilagewith alkaline water; and salting out, removing free proteins and purifying the shark cartilage to obtain an active component of the shark cartilage. A pharmacological experiment displays that the shark cartilage glycoprotein has remarkable anti-angiogenesis action and also can inhibit proliferation of cells such as skin cancer, breast cancer, non-small cell lung cancer, liver cancer, gastric cancer and ovarian cancer. An in vivo experiment also shows an excellent anti-tumor effect, and the shark cartilage glycoprotein can be widely applied to preventing and treating cancers.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to a shark cartilage glycoprotein and its anticancer application. Background technique [0002] Cancer, also known as malignant tumor, refers to a type of disease in which cells proliferate uncontrollably caused by the abnormality of the mechanism of controlling cell proliferation, and locally invade the surrounding normal tissues, and even transfer to other parts of the body through the internal circulatory system or lymphatic system. . Because cancer has no obvious clinical symptoms in its early stage, it is often in the advanced stage when it is discovered, and the optimal treatment window period is often missed when it is discovered, resulting in a high fatality rate and becoming one of the five terminal diseases in the world. my country is one of the areas with a high incidence of cancer, and the incidence of cancer has been increasing year by year in recent years. In 2014...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/02A61K35/60A61K36/90A61K36/898A61K9/06A61P35/00A61K35/618A61K35/586A61K35/32A61K33/00
CPCA61K9/0014A61K9/06A61K33/00A61K35/32A61K35/586A61K35/60A61K35/618A61K36/185A61K36/36A61K36/481A61K36/78A61K36/84A61K36/896A61K36/898A61K36/90A61K38/02A61P35/00A61K2300/00
Inventor 车路平
Owner 车路平
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