Method of utilizing graphene oxide (GO)/eosin Y compound to photo-catalytically degrade beta-lactam type antibiotic in water
A technology of lactams and antibiotics, applied in the field of environmental management, can solve the problems of low removal efficiency, harsh operating conditions, narrow application range, etc., and achieve the effect of simple removal method
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Embodiment 1
[0013] Add 50 mg L sequentially to a clean 24-well culture plate -1 80 μL of cefaclor (CEC) solution, 1720 μL of pH 7.0 PBS buffer solution, 0.1 mg mL -1 The graphene oxide / eosin Y (GO / EY) dispersion was 200 μL, and the total volume was 2 mL. The multi-well plate was incubated in the dark for 45 min, and then irradiated with a white LED light for 4 h. Replenish to the volume before irradiation with ultrapure water, centrifuge, measure the absorbance of the supernatant at a wavelength of 264 nm with an ultraviolet spectrophotometer, and calculate the removal efficiency.
Embodiment 2
[0015] Add 50 mg L sequentially to a clean 24-well culture plate -1 80 μL of cefaclor (CEC) solution, 1720 μL of pH 7.0 PBS buffer solution, 0.1 mg mL -1 The graphene oxide / eosin Y (GO / EY) dispersion was 200 μL, and the total volume was 2 mL. The multi-well plate was incubated in the dark for 45 min, and then irradiated with a green LED light for 4 h. Replenish to the volume before irradiation with ultrapure water, centrifuge, measure the absorbance of the supernatant at a wavelength of 264 nm with an ultraviolet spectrophotometer, and calculate the removal efficiency.
Embodiment 3
[0017] Add 50 mg L sequentially to a clean 24-well culture plate -1 80 μL of cefaclor (CEC) solution, 1720 μL of pH 7.0 PBS buffer solution, 0.1 mg mL -1 The graphene oxide / eosin Y (GO / EY) dispersion was 200 μL, and the total volume was 2 mL. The multi-well plate was incubated in the dark for 45 min, and then irradiated with a white LED light for 6 h. Replenish to the volume before irradiation with ultrapure water, centrifuge, measure the absorbance of the supernatant at a wavelength of 264 nm with an ultraviolet spectrophotometer, and calculate the removal efficiency.
[0018] Using Examples 1, 2, and 3 to treat environmental water samples 1, 2, and 3 respectively, the removal effect is better, and the results are shown in Table 1.
[0019] Table 1 The removal results of cefaclor (CEC) in actual water samples
[0020]
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