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Mycoplasma gallisepticum high density fermentation culture technology

A high-density fermentation technology of Mycoplasma gallisepticum, which is applied in the field of agricultural microorganisms, can solve problems such as the difficulty of large-scale production of Mycoplasma gallisepticum, and achieve the effects of improving the protection effect, improving immunogenicity, and increasing the density of viable bacteria

Active Publication Date: 2018-12-07
兆丰华生物科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The main purpose of the present invention is to provide a high-density fermentation and cultivation process of Mycoplasma gallisepticum, which is used to solve the problem that Mycoplasma gallisepticum is difficult to produce on a large scale, can increase the density of live bacteria at the end of fermentation, improve the immunogenicity of bacterial strains, and improve the production efficiency of vaccines. protective effect

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  • Mycoplasma gallisepticum high density fermentation culture technology
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  • Mycoplasma gallisepticum high density fermentation culture technology

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Embodiment 1

[0022] A kind of Mycoplasma gallisepticum high-density fermentation culture technique provided by the present embodiment 1 comprises the following steps:

[0023] Step 1: Take the freeze-dried seeds of Mycoplasma gallisepticum for production and inoculate them in the culture medium of Mycoplasma gallisepticum at a ratio of 1:10, and culture them for 18-24 hours under the condition of 36-37°C to obtain the culture medium, and in the same way The obtained culture medium was inoculated and passaged again at a ratio of 1:10;

[0024] Step 2: Inoculate the bacterium liquid after the above-mentioned subculture into the sterilized Mycoplasma gallisepticum culture medium of a 20L fermenter at a ratio of 3% / V / V, and the cultivation process conditions of the fermenter are: the temperature is 37°C, and the tank pressure is 0.03Mpa, the dissolved oxygen is 40%, the pH value is 7.7, and the stirring speed is 100rpm. During the cultivation process, when adding 20% ​​NaOH solution online to ...

Embodiment 2

[0030] A kind of Mycoplasma gallisepticum high-density fermentation culture process that present embodiment 2 provides, comprises the following steps:

[0031] Step 1: Take the freeze-dried seeds of Mycoplasma gallisepticum for production and inoculate them in the culture medium of Mycoplasma gallisepticum at a ratio of 1:10, and culture them for 18-24 hours under the condition of 36-37°C to obtain the culture medium, and in the same way The obtained culture medium was inoculated and passaged again at a ratio of 1:10;

[0032] Step 2: Inoculate the bacterium liquid after the above-mentioned subculture into the sterilized Mycoplasma gallisepticum culture medium of a 20L fermenter at a ratio of 4% / V / V, and the cultivation process conditions of the fermenter are: the temperature is 37°C, and the tank pressure is 0.05Mpa, the dissolved oxygen is 40%, the pH value is 7.7, and the stirring speed is 100rpm. During the cultivation process, when adding 20% ​​NaOH solution online to con...

Embodiment 3

[0039] A kind of Mycoplasma gallisepticum high-density fermentation culture technique provided by the present embodiment 3 comprises the following steps:

[0040] Step 1: Take the freeze-dried seeds of Mycoplasma gallisepticum for production and inoculate them in the culture medium of Mycoplasma gallisepticum at a ratio of 1:10, and culture them for 18-24 hours under the condition of 36-37°C to obtain the culture medium, and in the same way The obtained culture medium was inoculated and passaged again at a ratio of 1:10;

[0041]Step 2: Inoculate the bacterium liquid after the above-mentioned subculture into the sterilized Mycoplasma gallisepticum culture medium of a 20L fermenter at a ratio of 3% / V / V, and the cultivation process conditions of the fermenter are: the temperature is 37°C, and the tank pressure is 0.03Mpa, the dissolved oxygen is 40%, the pH value is 7.7, and the stirring speed is 100rpm. During the cultivation process, when adding 20% ​​NaOH solution online to c...

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Abstract

The invention discloses a mycoplasma gallisepticum high density fermentation culture technology, and belongs to the field of agriculture microbes. A chicken bursa mycoplasma fermentation high densityantigen is obtained through following steps: resuscitating freeze-dried strains in a mycoplasma gallisepticum culture medium, and then carrying out inoculum enlargement and fermentation culture. During the fermentation process, a mycoplasma gallisepticum culture medium is added, the pH is adjusted, industrial amplify production is carried out, enough nutrients are provided for mycoplasma gallisepticum, the production cost is reduced, the antigen content is high, and the technology is suitable for large scale application and has a wide application prospect.

Description

technical field [0001] The invention relates to a high-density fermentation and cultivation process of mycoplasma gallisepticum, belonging to the field of agricultural microorganisms. Background technique [0002] Mycoplasma gallisepticum MG is the pathogen of chicken chronic respiratory disease CRD, and the infection rate is very high. CRD can spread through horizontal and vertical ways and exist and spread for a long time in chicken flocks. After chicken farms are infected with MG, the rate of weak chicks in chickens increases , the egg production rate of laying hens, the weight of broilers and the feed conversion rate all decreased, which caused serious harm to the development of the chicken industry. At present, the treatment of Mycoplasma gallisepticum in various chicken farms is mainly to use antibiotics, and Mycoplasma gallisepticum is easy to produce Drug resistance, the effect is not satisfactory, immunization vaccine is the best measure to control mycoplasma gallis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/35
CPCC12N1/20
Inventor 丁美娟张小飞尹秀凤卢凤英严鹏许秀梅汪爱芬
Owner 兆丰华生物科技(南京)有限公司
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