Fixation treatment method for in-vitro breast cancer axillary lymph node tissue specimen

A technology of tissue samples and processing methods, which is applied in the field of fixed processing of isolated tissue samples of breast cancer axillary lymph nodes. Permeability, accurate transfer positive rate, and the effect of improving the quality of samples

Inactive Publication Date: 2018-12-07
SHAOXING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For radical tumor resection specimens such as gastric cancer, colon cancer, and breast cancer, thorough lymph node sampling is crucial for tumor diagnosis, staging, formulation of treatment plans, and prognosis, but conventional fixative solutions and existing specimen fixation methods cannot meet the requirements. Impro

Method used

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  • Fixation treatment method for in-vitro breast cancer axillary lymph node tissue specimen
  • Fixation treatment method for in-vitro breast cancer axillary lymph node tissue specimen
  • Fixation treatment method for in-vitro breast cancer axillary lymph node tissue specimen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: different compositions of ointment fixative

[0021] The core functional components of the palmitate fixative solution provided by this application are mainly formaldehyde, lecithin, absolute ethanol and acetone, and in different implementation environments, deoxycholic acid, methanol, PBS solution and any One or more, the specific composition selection method can refer to Table 1.

[0022] Table 1 different compositions of ointment fixative

[0023]

[0024]

[0025] We tested the core components of the ointment fixative (i.e. No. 1 in Table 1) and supplementary components respectively, and the above 16 situations can be obtained. The same components were tested in parallel with the same samples at the same concentration, and the results showed that: The soft lipid fixative of the core component has a good fixation effect on adipose tissue, organ tissue (such as thyroid tissue, breast tissue, lung tissue) or colon tissue, and the lymph nodes are we...

Embodiment 2

[0028] Embodiment 2: the same composition different concentrations of ointment fixed solution

[0029] The present embodiment provides the composition and concentration of the palmitate fixative solution of the whole composition (i.e. including all core constituents and all supplementary constituents), and several representative groups are listed as shown in Table 2:

[0030] Table 2 different concentrations of palmitate fixative

[0031]

[0032] The pretreated surgically resected omentum specimens were treated with the omentum fixative solution corresponding to the serial numbers in Table 2 for 4 hours, and then the lymph nodes were collected, dehydrated, embedded, sectioned, stained, immunohistochemical, and observed under a microscope. The results showed that: The above-mentioned concentrations can dissolve the sample to varying degrees, the fat is soft and liquefied, the lymph nodes are effectively exposed, the cell morphology of the lymph nodes is preserved intact, an...

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Abstract

The present application relates to a fixation treatment method for an in-vitro breast cancer axillary lymph node tissue specimen, and belongs to the technical field of sample preparation for testing.A to-be-tested in-vitro breast cancer axillary lymph node dissection tissue specimen is immersed in a stationary liquid for 2 to 24 hours. After washing, the specimen is immersed in a hydrochloric acid solution for 30 to 120 minutes, then rinsed, and soaked in an alkali solution for 30 to 120 minutes. A digestive solution contains 0.1-2 mg/ml of protease K, 1-100 mg/ml of trypsin and 0.1-2 mg/ml of EDTA, and after constant temperature water bath treatment for 1-10 hours, lymph nodes are cut, routinely dehydrated, embedded, sliced and stained to complete the treatment. The fixation treatment method for the in-vitro breast cancer axillary lymph node tissue specimen can not only soften and dissolve adipose tissue effectively, and ensures timely and sufficient fixation of the lymph nodes, andthe number of detected lymph nodes and accurate lymph node metastasis positive rate are effectively improved.

Description

technical field [0001] The application relates to a method for fixing and processing isolated tissue samples of axillary lymph nodes of breast cancer, and belongs to the technical field of test sample preparation. Background technique [0002] Existing fresh isolated specimens are usually soaked in formalin fixative solution, mainly because formaldehyde can preserve the morphological and structural characteristics of tissues for a long time and has good economic utility at the same time. Although this type of specimen processing method can well fix the shape of the tissue, it also hardens the specimen containing adipose tissue, which greatly increases the difficulty in obtaining materials in pathology departments, especially lymph nodes. For radical tumor resection specimens such as gastric cancer, colon cancer, and breast cancer, thorough lymph node sampling is crucial for tumor diagnosis, staging, formulation of treatment plans, and prognosis, but conventional fixative sol...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/305
Inventor 李韩鑫魏建国蒋雄玲陈洋张剑孙爱静
Owner SHAOXING UNIVERSITY
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