Culture method of rape isolated microspore regenerated plants

Abstract

The invention belongs to the technical field of plant tissue culture, and discloses a culture method of rape isolated microspore regenerated plants. The culture method comprises the following steps ofsoaking the root of the regenerated plant cultured by a water culture method in a colchicine solution with certain concentration, wherein the height of the regenerated plant is 15 to 25cm; continuingto culture the regenerated plant in water, and transplanting into a field. The culture method has the advantages that the regenerated plant after water culture is strong, the root is developed, and the vigor of the root is strong; when the root is soaked by the colchicine solution, the root can fully and uniformly absorb the components of the colchicine, and the doubling effect is better; the seedling digging and root washing are not required, the root is not damaged in the whole process, the survival rate of the regenerated plant is improved, the operation is simple and is easy to implement,the implementing is easy, the cost is low, and the culture method is suitable for being produced and applied in a large-scale way.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for cultivating rapeseed microspore regenerated plants in vitro. Background technique [0002] my country is a big oil consumption country in the world. The supply of domestic edible vegetable oil has been seriously insufficient for a long time, the import pressure remains high, and safety hazards are becoming increasingly prominent. Rapeseed oil is the largest source of domestic edible vegetable oil, accounting for more than 50% of the oil production of domestic oil crops. Therefore, the stable and healthy development of the rapeseed industry is an important guarantee for the safety of vegetable oil supply in my country. [0003] The high-yield and high-quality development of the rapeseed industry is inseparable from the support of excellent varieties. In the research and development of new rapeseed varieties, microspore culture technology plays a vital role,...

AI Technical Summary

Problems solved by technology

Among these three methods, the doubling rate of the colchicine root soaking method is the highest, but the traditional root soaking doubling method needs to dig out the haploid plants from the soil first, wash the root soil and use a certain concentration of colchicine solution Soak the roots for 2 to 6 hours, then rinse the roots with clean water and transplant them into the soil. The operation is cumbersome, especially for large-scale treatment, the workload of digging seedlings, washing roots, and transplanting is very large; secondly, the process of digging seedlings and transplanting In the middle, it is easy to damage the root system, which will have a very adverse effect on the growth of the plant.

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