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Application of LAE (ethyl lauroylarginate) as veterinary antibacterial agent

A technology of use and dosage, applied in the application field of lauroyl arginine ethyl ester as a veterinary antibacterial agent, can solve the problems such as uninstructed and unstudied LAE independent bacteriostatic effect and the like

Active Publication Date: 2018-12-14
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method involves multiple antibacterial components including LAE, and the antibacterial effect of LAE alone was not studied
At the same time, the invention only teaches that the composition is used for daily products, cleaning agents, wound care compositions, various foods, various medical cleaning products, etc., and does not teach how to use a single LAE component for poultry Use of antibacterial feed for aquatic products

Method used

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  • Application of LAE (ethyl lauroylarginate) as veterinary antibacterial agent
  • Application of LAE (ethyl lauroylarginate) as veterinary antibacterial agent
  • Application of LAE (ethyl lauroylarginate) as veterinary antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment one: micro-broth dilution method measures LAE to bacterium minimum inhibitory concentration (MIC)

[0074] Principle and purpose: According to the micro broth dilution method stipulated by CLSI, after co-incubating the drug and bacteria in a 96-well plate for 24 hours, the minimum drug concentration at which bacterial growth is inhibited is the minimum inhibitory concentration of the drug.

[0075] Methods: LAE and bacteria (the clinical isolate of Riemerella anatipestifer RA‐11, Staphylococcus aureus ATCC 29213, and Escherichia coli ATCC 25922) were diluted with tryptone soy broth (TSB) and cultured. Added to the 96-well plate, the blank control medium CK1 without bacteria, the medium CK2 added with LAE (1000 μg / ml) and the control medium CK3 without the drug LAE but with normal growth of bacteria were set up. The 96-well plate was placed in a 37° C. incubator and incubated for 24 h, and then the absorbance value at 625 nm of each well was measured. OD of b...

Embodiment 2

[0080] Embodiment two: LAE is to the mensuration of effective bactericidal time of bacteria

[0081] Principle and purpose: Drugs and bacteria are co-incubated, and some samples are taken out at regular intervals for bacterial plate counting, so as to obtain the growth curve of bacteria under the action of the drug, which is the time-killing curve of the drug.

[0082] Method: The bacteria in the logarithmic phase were mixed with different concentrations of LAE solutions, samples were taken at 0, 0.5, 1, 1.5, and 2 hours after the addition of the drug, and the 10-fold serial dilution points were placed on the TSA plate, and cultured at 37°C for 18‐ 24h. Calculate the bacterial concentration of each dosing group at each time point by the number of colonies, and draw as follows figure 1 Bacterial survival curves are shown.

[0083] Result analysis: at a concentration of 16 μg / ml, LAE can completely kill Riemerella anatipestifer and Escherichia coli within 30 minutes, and redu...

Embodiment 3

[0084] Example 3: LAE-induced bacterial drug resistance experiment

[0085] Principle and purpose: Bacteria can be induced to produce drug-resistant bacteria at sub-inhibitory concentrations, and the drug-induced bacteria can be detected by long-term transfer of bacteria cultured with sub-inhibitory concentrations of drugs to fresh drug-containing media Ability to generate resistance mutations.

[0086] Method: The bacteria in the logarithmic phase were mixed with the LAE solution in a glass test tube, sealed with a cotton plug, and placed in a constant temperature shaker at 37°C and 220 rpm for 24 hours. Afterwards, check the MIC of the drug every 24 hours, and add the bacterial solution with the highest drug concentration (that is, the highest drug concentration lower than the MIC) that can cultivate a turbid bacterial solution to fresh cultures containing different drug concentrations at a ratio of 1:100. In Kiri, culture at 37°C, 220rpm for 24h and last for 30 days. Chlo...

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Abstract

The invention relates to a novel veterinary antibacterial agent. The antibacterial agent comprises condensation products from fatty acid and esterified binary amino acids and can prevent and treat theRA (riemerella anatipestifer) disease of ducklings due to RA, treat the mouse peritonitis caused by escherichia coli and staphylococcus aureus and prevent fish septicemia caused by mild aeromonas. The antibacterial agent can effectively inhibit or kill bacteria, can substantially reduce the risk of bacterial infection of poultry, livestock and aquatic animals and increase the survival rate of animals that have infected with pathogenic bacteria. LAE can be safely decomposed in a human body or animal body, has small toxic and side effects, avoids production of environment drug-resistance bacteria caused by the fact that remaining antibacterial agents are discharged to the environment and has a small negative effect on the environment while realizing the effective antibacterial effect.

Description

[0001] The application of this invention requires Chinese invention patent application 201711071006.8, and the name of the invention is "lauroyl arginine Use of ethyl esters and their derivatives as veterinary antibacterial agents" priority application. technical field [0002] The invention relates to the application of the compound lauroyl arginine ethyl ester in the preparation of veterinary antibacterial agents, feed additives or feed nutritional energy substances. Background technique [0003] Riemerella anatipestifer is a contagious disease caused by Riemerella anatipestifer (RA), also known as duck infectious serositis, duck septicemia, duck plague syndrome, and duck plague. bacillosis etc. It is more common in ducklings of 1-8 weeks of age, and ducklings of 2-4 weeks of age are the most susceptible. It is acute or chronic sepsis, clinically manifested as increased eye and nasal secretions, panting, coughing, diarrhea, ataxia, and head and neck tremors, and a smal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/223A61P31/04A61P1/12A23K50/75A23K50/30A23K50/10A23K50/20A23K50/40A23K50/80A23K20/142
CPCA23K20/142A61K31/223A23K20/195
Inventor 易正芳邵婷范婷婷刘明耀
Owner EAST CHINA NORMAL UNIV
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