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a protective agent for 60 The method of cultivating new varieties of tulip by co-γ radiation mutagenesis

A protective agent and tulip technology, which is applied in the field of cultivating new varieties of tulips by using radiation mutation breeding technology, can solve the problems that have not yet been seen in the application research of protective agents, and achieve the effects of reducing the risk of short growth cycle, increasing yield, and increasing the number of flowering plants

Active Publication Date: 2021-11-02
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, there is no related research on the application of protective agents in radiation mutation breeding of bulbous flowers such as tulips

Method used

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  • a protective agent for  <sup>60</sup> The method of cultivating new varieties of tulip by co-γ radiation mutagenesis
  • a protective agent for  <sup>60</sup> The method of cultivating new varieties of tulip by co-γ radiation mutagenesis
  • a protective agent for  <sup>60</sup> The method of cultivating new varieties of tulip by co-γ radiation mutagenesis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: The effect of protective agent treatment on the survival rate of tulips after gamma ray irradiation:

[0038] This experiment was carried out in the multi-span greenhouse of the School of Life Science and Engineering, Southwest University of Science and Technology. use 60 Co-γ irradiated healthy tulip bulbs, the radiation dose was 5Gy, 10Gy, 20Gy, 40Gy, 60Gy, 80Gy, 100Gy, and the radiation dose was 2.57Gy / min. Two controls were set up in the experiment, one was unirradiated and unwatered bulbs (CK 1 ), the second is the bulbs soaked in deionized water for 30 minutes (CK 2 ). According to 3 different melatonin concentrations for each radiation dose (see Table 1-1 for specific melatonin formulations), soak for 30 minutes respectively, dry and plant. The tulip planting time is January 20th, planted in a 10cm*10cm*30cm black nutrient pot, the soil formula is special soil for plant cultivation: fermented organic substrate: leaf humus: vermiculite = 2:1:1:1 ...

Embodiment 2

[0051] Embodiment 2: The effect of protective agent treatment on tulip vegetative growth after gamma ray irradiation:

[0052] This experiment was carried out in the multi-span greenhouse of the School of Life Science and Engineering, Southwest University of Science and Technology. use 60 Co-γ irradiated healthy tulip bulbs, the radiation dose was 5Gy, 10Gy, 20Gy, 40Gy, 60Gy, 80Gy, 100Gy, and the radiation dose was 2.57Gy / min. Two controls were set up in the experiment, one was unirradiated and unwatered bulbs (CK 1 ), the second is the bulbs soaked in deionized water for 30 minutes (CK 2 ). According to 3 different melatonin concentrations for each radiation dose (see Table 2-1 for the specific melatonin formula), soak for 30 minutes respectively, dry and plant. The tulip planting time is January 20th, planted in a 10cm*10cm*30cm black nutrient pot, the soil formula is special soil for plant cultivation: fermented organic substrate: leaf humus: vermiculite = 2:1:1:1 (mixe...

Embodiment 3

[0062] Embodiment 3: the effect of protective agent treatment on tulip reproductive growth after gamma ray irradiation:

[0063] This experiment was carried out in the multi-span greenhouse of the School of Life Science and Engineering, Southwest University of Science and Technology. use 60 Co-γ irradiated healthy tulip bulbs, the radiation dose was 5Gy, 10Gy, 20Gy, 40Gy, 60Gy, 80Gy, 100Gy, and the radiation dose was 2.57Gy / min. Two controls were set up in the experiment, one was unirradiated and unwatered bulbs (CK 1 ), the second is the bulbs soaked in deionized water for 30 minutes (CK 2 ). According to 3 different melatonin concentrations for each radiation dose (see Table 1-1 for specific melatonin formulations), soak for 30 minutes respectively, dry and plant. The tulip planting time is January 20th, planted in a 10cm*10cm*30cm black nutrient pot, the soil formula is special soil for plant cultivation: fermented organic substrate: leaf humus: vermiculite = 2:1:1:1 (m...

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Abstract

The invention discloses a method of using a protective agent in 60 The method for cultivating new varieties of tulips by Co-γ radiation mutagenesis is characterized in that: the method will pass the dose rate of 2.57Gy / min 60 Co-gamma irradiated tulip bulbs are soaked in an aqueous solution of a protective agent, and then planted after being dried; the protective agent is any one or a mixture of melatonin, gibberellin, and salicylic acid . Further, during the tulip planting process, the tulip plants can be sprayed with the protective agent aqueous solution. By adopting the present invention, soaking and treating irradiated bulbs with a protective agent can reduce the damage to bulbs after radiation-induced mutation breeding, increase the germination rate and survival rate of bulbs, promote the normal growth of their vegetative and reproductive periods, and improve Variation rate: Secondary protection by spraying plants can improve the seedling formation and fertility of tulips, increase the number of flowering plants, increase the variation rate of flowers, and help to breed new tulip varieties in a short period of time.

Description

technical field [0001] The invention belongs to a method for obtaining new plants, and relates to a method of using a protective agent in 60 The method for cultivating new varieties of tulips by Co-γ radiation mutation is particularly suitable for cultivating new varieties of tulips by means of radiation mutation breeding technology. Background technique [0002] With the development and wide application of nuclear technology, the opportunities for people to be exposed to radiation are increasing day by day. The application of radioprotectants has attracted much attention. Researchers have been devoting themselves to the study of highly efficient, low-toxic natural radiation protectors. [0003] Since Muller [Muller H J. X-ray mutation generation in 1927 [J]. Proceedings of the National Academy of Sciences of the United States, 1928, 14 (9): 714-726.] discovered the mutagenic effect of radiation on organisms, ionizing radiation mutagenesis technology has been Used in plan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06A01C1/00A01G22/35A01G7/06
CPCA01C1/00A01G7/06A01H1/06A01G22/35
Inventor 王丹黎熠睿湛晓蝶刘玲闵可怜
Owner SOUTHWEAT UNIV OF SCI & TECH