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Cell preparation for treating ''female athlete triad'' and repairing ovarian function of female athletes and preparation method thereof

A cell preparation and athlete's technology, applied in cell dissociation methods, animal cells, vertebrate cells, etc., can solve problems affecting athletes' health, estrogen decline, bone density reduction, etc., and achieve considerable clinical application prospects and good curative effect Effect

Active Publication Date: 2018-12-18
徐妍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Amenorrhea can lead to a drop in estrogen, and the drop in estrogen and malnutrition can also reduce bone density, affecting the health of athletes

Method used

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  • Cell preparation for treating ''female athlete triad'' and repairing ovarian function of female athletes and preparation method thereof
  • Cell preparation for treating ''female athlete triad'' and repairing ovarian function of female athletes and preparation method thereof
  • Cell preparation for treating ''female athlete triad'' and repairing ovarian function of female athletes and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0050] The preparation of umbilical cord and placental mesenchymal stem cells refers to the following literature:

[0051] Comparative study of regenerative effects of mesenchymal stem cells derived from placental amnion, chorion and umbilical cord on dermal wounds[J].Placenta,2018,65.Ertl J,Pichlsberger M,Tuca A C,et al.

[0052] Bone densitometry experiment, refer to the following literature:

[0053] Bone mass of female dance students prior to professional dancetraining: A cross-sectional study[J].Plos One,2017,12(7):e0180639. Amorim T,Metsios G S,Wyon M,et al.

[0054] Low Bone Mineral Density in Male Athletes Is Associated With BoneStress Injuries at Anatomic Sites With Greater Trabecular Composition[J].American Journal of Sports Medicine,2017,46(3):363546517730584. Tenforde A S,Parziale A L,Popp K L,et al.

[0055] For female physiological measurement experiments, refer to the following documents:

[0056] Increasing training load without risking the female athlete tri...

Embodiment 1

[0059] Example 1 Preparation of umbilical cord mesenchymal stem cells fully domesticated by rhSDF-1α and IGF-II

[0060] The umbilical cords of newborns delivered by cesarean section in full-term healthy pregnant women were collected and fully washed. Cut the tissue into pieces, add collagenase digestion solution, shake and digest in a constant temperature shaker at 37°C for 30 minutes, and stop digestion with complete medium (containing 10% FBS). Filter the digested tissue with a 50-mesh sieve to obtain suspension cell tissue. The suspended cell tissue was added to the buffer, centrifuged and washed 3 times, and the culture medium was added to inoculate the culture dish. Place at 37°C, 5% CO 2 cultured in an incubator.

[0061] After the cells adhered to the wall, the medium was changed, culture medium containing 5% FBS was added, and 100ng / mL rhSDF-1α and 200ng / ml IGF-II were added for acclimatization, and corresponding cytokines were replenished in time when the medium w...

Embodiment 2

[0063] Example 2 Preparation of placental mesenchymal cells fully domesticated by rhSDF-1α and IGF-II

[0064] The placental tissues of term cesarean section of healthy and qualified pregnant women were collected, and the blood clots were fully washed away. Cut the tissue into blocks, remove the connective tissue, add collagenase digestion solution, shake and digest in a constant temperature shaker at 37°C for 30-40 minutes, and complete the medium (containing 10% FBS) to stop the digestion. Filter the digested tissue with a 50-mesh sieve to obtain suspension cell tissue. The suspended cell tissue was added to the buffer, centrifuged and washed 3 times, and the culture medium was added to inoculate the culture dish. Place at 37°C, 5% CO 2 cultured in an incubator.

[0065] The domestication culture method is the same as that in Example 1.

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Abstract

The present invention provides a cell preparation for treating ''female athlete triad'' and repairing ovarian function of female athletes. The cell preparation is composed of mesenchymal stem cells fully domesticated by rhSDF-1alpha and IGF-II, and normal saline used for carrying the mesenchymal stem cells. The mesenchymal stem cells are umbilical cord mesenchymal stem cells or placenta-derived mesenchymal stem cells. The preparation is introduced into a patient by intravenous drip, and symptoms of ''female athlete triad'' are improved by the immunomodulatory and hormonal regulation effects ofstem cells. The cell preparation can repair ovarian injury of athletes with ''female athlete triad'' and has a curative effect on rehabilitation of the athletes. A preparation method of the preparation provided by the invention is simple, easy to implement, safe and effective.

Description

technical field [0001] The invention belongs to the field of sports and health sciences, and relates to a cell preparation for treating "female athlete triad" and a preparation method thereof. Background technique [0002] The concept of "Female Athlete Triad" (Female Athlete Triad, FAT) was proposed by the American College of Sports Medicine (ACSM) in 1992, and specifically includes eating disorders (eating disorders), functional hypothalamic amenorrhea, and osteoporosis. The three elements of the triad are interrelated, and studies have found that even athletes with oligomenorrhea have very low bone density. Amenorrhea will lead to a drop in estrogen, and the drop in estrogen and malnutrition will also reduce bone density and affect the health of athletes. Eating disorders include severe eating disorders and atypical eating disorders. This problem has become a serious problem among athletes and even the general public. It is currently believed that the occurrence of this ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/10A61P1/14A61P15/00A61P5/02A61P19/10A61P15/08C12N5/0775
CPCA61K9/0019A61K9/10A61K35/28A61P1/14A61P5/02A61P15/00A61P15/08A61P19/10C12N5/0665C12N2501/105C12N2501/21C12N2509/00
Inventor 徐妍王海亚郭世磊
Owner 徐妍
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