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Kit and application of numb gene for screening lactation performance of Kazakh horses

A technology of lactation performance and kit, applied in the identification field of animal genetic selection and breeding, to achieve good specificity, improve lactation level, and accelerate the effect of breeding

Active Publication Date: 2021-12-31
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no studies and reports on the screening of lactation performance of Kazakh horses using kit technology at home and abroad, which is completely blank; in addition, for the NUMB gene as a candidate gene affecting the lactation performance of Kazakh horses, the relevant research reports are more common in In humans and mice, rarely reported in horses, no application studies in Kazakh horses

Method used

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  • Kit and application of numb gene for screening lactation performance of Kazakh horses
  • Kit and application of numb gene for screening lactation performance of Kazakh horses
  • Kit and application of numb gene for screening lactation performance of Kazakh horses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: The present invention is used to detect the lactation performance kit of Kazakh horses

[0051] The invention provides a detection kit for detecting lactation performance of Kazakh horses of NUMB gene, wherein the allele types of NUMB gene include GA and GG.

[0052] In the present invention, allelic types GA and GG were detected from Kazakh horse plasma.

[0053] The present invention also provides a detection kit containing NUMB gene for screening the lactation performance of Kazakh horses. 31. Tip area 32, blood collection tube area 33, DNA extraction reagent area 34, PCR amplification reagent area 35 and Marker area 36, ​​96-well plate area 31, tip area 32, blood collection tube area 33, DNA extraction reagent area 34 , PCR amplification reagent area 35 and Marker area 36 are all provided with sub-covers 2, and label 2 21 is set on sub-covers 2.

[0054] In the present invention, the box body 1 is cylindrical, the center column 4 is arranged in the midd...

Embodiment 2

[0065] Example 2: The present invention is used to detect the lactation performance kit of Kazakh horses

[0066] The present invention provides a detection kit containing NUMB gene for screening the lactation traits of Kazakh horses. The box body 1 is cylindrical, and a central column 4 is arranged in the middle of the box body 1, and a shaft hole 42 is arranged at the top of the central column 4, and the cover is divided into two parts. 2. A rotating shaft 41 adapted to the shaft hole 42 is provided at the bottom, so that the sub-cover 2 can be rotated and placed on other sub-covers 2 after opening, without occupying space. The partition 3 and the sub-cover 2 make each area airtight and divided , will not interfere with each other.

[0067] In the present invention, the 96-well plate area 31, the tip area 32, the blood collection tube area 33, the DNA extraction reagent area 34, the PCR amplification reagent area 35 and the Marker area 36 are all fan-shaped areas, and the su...

Embodiment 3

[0072] Example 3: Application of a detection kit containing NUMB gene for improving lactation performance of Kazakh horses

[0073] The present invention provides the application of the test kit containing NUMB gene for improving the lactation performance of Kazakh horses, which is embodied by the detection method and specifically includes the following steps:

[0074] (1) Extraction of tissue sample genomic DNA:

[0075] Collect 5ml of jugular venous blood of the Kazakh horse to be tested in vivo, use a blood collection tube filled with sodium citrate anticoagulant, store at -20°C, use the kit provided in the above example to extract genomic DNA, store at -20°C for later use, and The results of DNA extraction are attached Figure 4 shown.

[0076] (2) NUMB gene PCR amplification:

[0077] According to the designed primers, use the kit provided in the above examples to specifically amplify the NUMB gene; the PCR reaction system is: 2×Master Mix 2.5 μl, each 0.07 μl of the u...

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Abstract

The invention discloses a NUMB gene testing kit for screening the lactation performance of Kazakh horses and its application. The detection kit of the invention is used to improve the lactation performance of Kazakh horses by detecting GA and GG alleles. There are six detection kits. Each area, including the 96-well plate area, pipe tip area, blood collection tube area, DNA extraction reagent area, PCR amplification reagent area and Marker area, is a fan-shaped area. According to the self-designed specific primers, PCR is performed using the sample genomic DNA as a template Amplify, carry out KASP typing after the PCR amplification product; The present invention not only fills up the blank of this research field at home and abroad, but also is helpful for improving the lactation level of Kazakh horses, improving the economic income of farmers and herdsmen, and speeding up Xinjiang dairy horse industry. The development has practical application value and provides a scientific service platform for the sustainable development of animal husbandry in Xinjiang.

Description

technical field [0001] The invention relates to an identification method for animal genetic selection, specifically, the invention relates to the technical field of a kit for screening Kazakh horse lactation performance. Background technique [0002] As one of the five major pastoral areas, Xinjiang has abundant resources. Kazakh horses are native to Xinjiang and are mainly distributed in the northern foothills of Tianshan Mountains, west of the Junggar Basin and the western section of the Altay Mountains. The breed has relatively stable heredity and certain breed characteristics. It has a medium size and a rough and strong physique suitable for military supplies and civilian use. It has a sensitive body, a certain speed and endurance, and has a certain meat production and lactation ability. Horse milk has high nutritional value and special health care function, and is favored by more and more consumers, and people's demand for high-yield milk horses continues to increase. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12Q1/6888C12Q1/6858
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/158C12Q2600/156C12Q2600/172C12Q2535/125C12Q2531/113
Inventor 刘武军刘玲玲方超何美升
Owner XINJIANG AGRI UNIV