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SNP molecular marker related to body length of Macrobrachium rosenbergii and dactylopodite length of second paraeiopod and application thereof

A technique of Macrobrachium rosenbergii and molecular markers, applied in the field of molecular marker breeding, to achieve the effects of saving production costs, large economic application value and scientific research value, and accelerating genetic progress

Inactive Publication Date: 2018-12-18
FOSHAN UNIVERSITY
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the breeding area of ​​Macrobrachium rosenbergii in China is as high as more than 600,000 mu, but the number of fine species of Macrobrachium rosenbergii is far from meeting its huge industrial scale and huge seed demand. Therefore, the early breeding of Macrobrachium rosenbergii is very urgent and significant

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  • SNP molecular marker related to body length of Macrobrachium rosenbergii and dactylopodite length of second paraeiopod and application thereof
  • SNP molecular marker related to body length of Macrobrachium rosenbergii and dactylopodite length of second paraeiopod and application thereof
  • SNP molecular marker related to body length of Macrobrachium rosenbergii and dactylopodite length of second paraeiopod and application thereof

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Embodiment

[0025] 1. Detection of SNP molecular markers

[0026] 1. Design primers: design primer pairs that specifically amplify the gene fragment containing the T658C site of the Macrobrachium rosenbergii ND1 gene.

[0027] Utilize the software Primer 5.0, use the ND1 gene (GenBank: NC_006880.1) of Macrobrachium rosenbergii in GenBank as the specificity amplification primer of reference design, the primer sequence that amplifies is

[0028] Upstream F: 5'-GATAAAGTAGGCTATGCTGG-3' (SEQ ID №1), 20bp;

[0029] Downstream R: 3'-CCTCCTCTGTACTCGGTATT-5' (SEQ ID No. 2), 20 bp.

[0030] The optimum temperature is 48.8°C, the product length is 542bp, and it was synthesized by Guangzhou Sangon Bioengineering Co., Ltd.

[0031] 2. Extraction of Macrobrachium rosenbergii Genomic DNA

[0032] Using OMEGAD whole blood kit, the specific steps are as follows:

[0033] (1) Take 150 μL blood sample for each sample, add Elution Buffer, and vortex for 10 seconds;

[0034] (2) Add 500 μL of Buffer GL l...

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Abstract

The invention discloses a SNP molecular marker related to the body length of Macrobrachium rosenbergii and the dactylopodite length of the second paraeiopod and application thereof in the technical field of molecular marker breeding. The SNP molecular marker corresponds to the T658C site of the ND1 gene of Macrobrachium rosenbergii, wherein the base is T or C. The SNP molecular marker of the invention is related to the body length of Macrobrachium rosenbergii and the dactylopodite length of the second paraeiopod, and is a new molecular marker. By determining the genotype of the SNP site of Macrobrachium rosenbergii to be tested, early breeding can be carried out according to the body length of Macrobrachium rosenbergii and the dactylopodite length of the second paraeiopod. Thereby, production costs can be effectively saved and genetic progress can be accelerated, better serving the breeding of Macrobrachium rosenbergii. The SNP molecular marker can satisfy the huge market demand and has great economic application value and scientific value.

Description

technical field [0001] The invention belongs to the technical field of molecular marker breeding, in particular to a SNP molecular marker related to the body length of Macrobrachium rosenbergii and the length of the second phalanx and its application. Background technique [0002] Marker Assisted Selection (MAS) uses the characteristics of molecular markers closely linked with genes that determine target traits, and screens important economic traits (such as weight, growth rate, stress resistance, etc.) in the process of animal and plant breeding. Related molecular markers, and construct a breeding population with related molecular markers in the breeding process to achieve the purpose of selecting target traits. It has the characteristics of strong targeting and high accuracy, and can greatly improve Breeding efficiency, shorten the breeding period. [0003] Single Nucleotide Polymorphisms (Single Nucleotide Polymorphisms, SNP) is a base variation produced by a single nucl...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 冯艺陈文强李浩杰袁启志李镇养刘咸张志浩陈文坚杨虹陈建酬
Owner FOSHAN UNIVERSITY