Novel method for analyzing tyrosinase activity using carbon quantum dots as fluorescence probes

Abstract

The invention discloses a novel method for analyzing tyrosinase activity using carbon quantum dots as fluorescence probes, characterized in that when an assay system contains only tyrosinase or dopamine ,the fluorescence of the carbon quantum dots remains stable; and when the assay system contains tyrosinase and dopamine simultaneously, the tyrosinase catalyzes the oxidation of dopamine to producequinonoids, resulting in quenching of the fluorescence of the carbon quantum dots; based on which a fluorescence analysis method for detecting tyrosinase is established. The novel method for analyzing tyrosinase activity using carbon quantum dots as fluorescence probes has the advantages of being fast, sensitive and simple. Further, the quenching of the fluorescence of the carbon quantum dots hasa good linear correlation with the concentration of tyrosinase, and the detection limit is as low as 0.039 U / mL. Meanwhile, a recovery rate of tyrosinase in actual human serum samples is in a range from 93.3% to 112.7%. Therefore, the novel method for analyzing tyrosinase activity using carbon quantum dots as fluorescence probes is expected to be an effective method for clinically detecting tyrosinase.

Description

Technical field [0001] The invention relates to the application field of carbon nanomaterials, in particular to a fluorescence analysis method based on carbon quantum dots to detect the activity of tyrosinase. Background technique [0002] (1) Tyrosinase (TYR) is a copper-containing polyphenol oxidase that is widely present in animals, plants and even microorganisms. It is a vital enzyme for organisms, and plays a vital role in many physiological processes such as plant browning reaction, mammalian insect cuticle hardening, and melanogenesis. As a copper-containing oxidase, the properties of tyrosinase depend on the oxidation state of two copper atoms in the active site, and exhibit two catalytic properties—monooxygenase and oxidase activity. At present, the detection of tyrosinase activity at home and abroad is mostly focused on colorimetry or potentiometric method, and there are few reports on fluorescence detection of tyrosinase. Among them, although the traditional colorime...

AI Technical Summary

Problems solved by technology

However, due to the instability of intermediate products, the accuracy and sensitivity of the detection are hindered

Although the electrochemical method has the advantages of high sensitivity, it is easily disturbed by the environment, and the reproducibility of detection is poor.

Images