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Callus induction method of amomum villosum rhizome buds

A technology of callus and rhizome, which is applied in the field of callus induction, can solve the problems of low reproduction coefficient and limited use time, and achieve the effect of abundant sources, stable medicinal properties, and not easy to mutate

Inactive Publication Date: 2018-12-21
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the former type of work has not been induced by callus, its reproduction coefficient is low, and it is impossible to use callus for further research such as gene transformation.
The latter type of work uses seeds or seeds to germinate seedlings as explants, and the availability of seeds is limited by seasons, and the use time is limited

Method used

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  • Callus induction method of amomum villosum rhizome buds
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  • Callus induction method of amomum villosum rhizome buds

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A method for inducing the callus of Yangchunsha rhizome buds, comprising the following steps:

[0032] Select healthy field-cultivated rhizome buds of Yangchunsha, cut them into small sections of about 3 cm after aseptic operation and disinfection, cut them longitudinally into two petals of similar size along the central axis, and then place them horizontally Inoculate in the prepared and sterilized medium for cultivation, inoculate 20 bottles, observe and record every 3 days. The well-growing callus was selected by culture, and the successfully induced callus was subcultured once every 30 days.

[0033] The environmental conditions for cultivation are as follows: temperature: 22°C; relative air humidity: 50%; light control is 10 hours of light, and light intensity is 1500Lx.

[0034] The medium formula is: MS+0.5mg / L 6-BA+0.1mg / L 2,4-D+0.05mg / L NAA

Embodiment 2

[0036] A method for inducing the callus of Yangchunsha rhizome buds, comprising the following steps:

[0037] Select healthy field-cultivated rhizome buds of Yangchunsha, cut them into small sections of about 3 cm after aseptic operation and disinfection, cut them longitudinally into two petals of similar size along the central axis, and then place them horizontally Inoculate in the prepared and sterilized medium for cultivation, inoculate 20 bottles, observe and record every 3 days. The well-growing callus was selected by culture, and the successfully induced callus was subcultured once every 30 days.

[0038] The environmental conditions for cultivation are as follows: temperature: 25°C; relative air humidity: 80%; light control is 15 hours of light, and light intensity is 2000Lx.

[0039] The medium formula is: MS+2.5mg / L 6-BA+2.0mg / L 2,4-D+2.0mg / L NAA

Embodiment 3

[0041] A method for inducing the callus of Yangchunsha rhizome buds, comprising the following steps:

[0042] Select healthy field-cultivated rhizome buds of Yangchunsha, cut them into small sections of about 3 cm after aseptic operation and disinfection, cut them longitudinally into two petals of similar size along the central axis, and then place them horizontally Inoculate in the prepared and sterilized medium for cultivation, inoculate 20 bottles, observe and record every 3 days. The well-growing callus was selected by culture, and the successfully induced callus was subcultured once every 30 days.

[0043] The environmental conditions for cultivation are as follows: temperature: 22°C; relative air humidity: 80%; light control is 15 hours of light, and light intensity is 1800Lx.

[0044] The medium formula is: MS+1.0mg / L 6-BA+0.5mg / L 2,4-D+0.1mg / L NAA

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Abstract

The invention provides a callus induction method of amomum villosum rhizome buds. Rhizome buds of amomum villosum are used as explants to be sterilized, inoculated and induce cultured to obtain callus, the induction culture medium is MS+0.5 to 2.5 mg / L 6-BA+0.1 to 2 mg / L 2,4-D+0.05 to 2 mg / L NAA, the illumination time of a culture environment is 10 h, the illumination intensity is 2000 Lx, and theculture temperature is 25 DEG C. The rhizome buds of the amomum villosum are used as the explants, the complete drug property of a parent can be ensured by adopting vegetative propagation, and no variation is likely to occur, so that the drug property is stable, the treatment effect of the drug can be ensured, the explant material is easy to obtain, and the explant material can be obtained at anytime for the propagation culture.

Description

technical field [0001] The invention relates to a callus induction method, which belongs to the technical field of tissue culture. Background technique [0002] Amomum villosum Lour. is a perennial evergreen herb of the genus Amomum Roxb. in the ginger family. It is used as medicine with its dried and ripe fruit. It is also known as "Amomum villosum Lour." , has a history of more than 1300 years of application. Spring amomum is warm in nature and pungent in taste. It has the effects of reducing dampness and appetizing, warming spleen and stopping diarrhea, regulating qi and preventing miscarriage. Yangchun sand is mainly distributed in Guangdong, Guangxi, Yunnan and other places, among which the amomum produced in Guangdong Yangchun has the best medicinal effect. Amomum has a large demand, and the annual demand is estimated to exceed 2.2×10 6 kg, due to the high fruit drop rate and low yield of Amomum edulis, the output of Amomum amomum is far from meeting the demand of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 何国振李明晓陈树辉徐杰何卓航林吉
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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