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Protectant for lyophilization preservation of phellinus linteus strains and preservation method thereof

A technology of P. clefthoofus and protective agent, which is applied in the field of protective agent for freeze-drying and preservation of P. clefthoofus strains, and can solve the problems of unfavorable protection and continuation of rare species.

Active Publication Date: 2018-12-21
广州汉方医学生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are very few studies on the preservation of P. clefthoofus strains, which is a major shortcoming for this rare medicinal fungus species, and it is also unfavorable for the protection and continuation of rare species.

Method used

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  • Protectant for lyophilization preservation of phellinus linteus strains and preservation method thereof
  • Protectant for lyophilization preservation of phellinus linteus strains and preservation method thereof
  • Protectant for lyophilization preservation of phellinus linteus strains and preservation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of sporulation medium

[0026] The first culture: wash wheat grains, soybeans, and black rice, weigh 10g each into an inoculation bottle, add 12g of deionized water, sterilize with high-temperature steam for 30min, and set aside;

[0027] The second culture: In addition, weigh 2.2g of glucose, 1.0g of glycine, 0.6g of potassium dihydrogen phosphate and 0.6g of magnesium sulfate, 0.22g each of glutamic acid, L-isoleucine, and asparagine, and cyclic adenylic acid 0.09g, vitamin 0.15g, dissolve it in 18g deionized water, use a 0.22μm sterile filter to filter and sterilize, and the pH is natural. The above vitamins are B vitamins 1 , Vitamin B 12 , Vitamin B 6 an equivalent composition of

[0028] The above two cultures were mixed uniformly under aseptic operation to obtain a sporulation medium.

[0029] (2) Sporulation culture

[0030] Under aseptic conditions, 0.8% of the weight of the culture medium was added to the above-mentioned spore-forming med...

Embodiment 2

[0049] (1) Preparation of sporulation medium

[0050] The first culture: wash wheat grains, soybeans, and black rice, weigh 13g each into an inoculation bottle, add 20g of deionized water, sterilize with high-temperature steam for 30min, and set aside;

[0051] The second culture: In addition, weigh 2.2g of glucose, 1.0g of glycine, 0.9g of potassium dihydrogen phosphate and 0.8g of magnesium sulfate, 0.25g each of glutamic acid, L-isoleucine, and asparagine, and cyclic adenylic acid 0.14g, vitamin 0.21g, dissolve it in 27g deionized water, use a 0.22μm sterile filter to filter and sterilize, and the pH is natural. The above vitamins are B vitamins 1 , Vitamin B 12 , Vitamin B 6 an equivalent composition of

[0052] The above two cultures were mixed uniformly under aseptic operation to obtain a sporulation medium.

[0053] (2) Sporulation culture

[0054] Under sterile conditions, add 1.0% of the weight of the culture medium to the above-mentioned spore-forming medium wi...

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Abstract

The invention relates to the field of preservation of phellinus linteus strains, in particular to a protectant for lyophilization preservation of phellinus linteus strains and a preservation method thereof. According to the technical solution, from the viewpoint of optimizing the protectant, a culture residue is added to the protectant, wherein the culture residue is obtained by sporulation culture of a sporulation medium of phellinus linteus, and drying and crushing of a culture left after spore extraction, and contains metabolites produced during sporulation of the phellinus linteus, including various oligosaccharides, peptides, amino acids, vitamins and other low molecular weight substances of which specific chemical compositions have not been discovered. The low molecular weight substances can protect cell membranes of the strains from being damaged in the external freezing environment, prevent cells from losing physiological activity to die due to freezing and drying so as to improve the survival rate of the freeze-dried strains.

Description

technical field [0001] The invention relates to the field of preservation of Phylofora clefthoof strains, in particular to a protective agent for freeze-dried preservation of Phylofora clefthoof strains and a preservation method thereof. Background technique [0002] Phylofora clefthoof is a precious folk traditional Chinese medicinal material, which has the effect of promoting blood circulation. New research has found that Phylonotus clefthoof has outstanding effects on inhibiting tumors and improving the body's immunity. Due to the scarcity of wild P. clefthoof resources, people began to domesticate the wild P. clefthooves strains and realize artificial cultivation to meet the market demand. A method for cultivating Phalopodium clefthoof on solid medium. [0003] In the actual production process of people, technicians have improved the production performance of the strains of P. clefthoofus, and obtained many batches of strains with excellent production performance. There...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/04C12N3/00C12R1/645
CPCC12N1/04C12N1/14C12N3/00
Inventor 涂桂洪王曙聘潘忠林谭志康祝廷霞
Owner 广州汉方医学生物科技有限公司
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