Application of alternaria alternata in preventing and controlling external invasion of weed solanum rostratum

A technology of thorny eggplant and foreign invasion, applied in the direction of application, chemicals for biological control, herbicides and algicides, etc., can solve the problems of single targeting, environmental pollution, time-consuming and labor-intensive, etc., and achieve good control effect , strong toxic lethal effect, strong pathogenic effect

Inactive Publication Date: 2018-12-25
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Artificial control can only work in a short period of time and in a small range, and it is time-consuming and laborious; chemical herbicides are prone to produce antibodies because of their single target, and chemical herbicides will pollute the environme...

Method used

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  • Application of alternaria alternata in preventing and controlling external invasion of weed solanum rostratum
  • Application of alternaria alternata in preventing and controlling external invasion of weed solanum rostratum
  • Application of alternaria alternata in preventing and controlling external invasion of weed solanum rostratum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Isolation, purification and screening of pathogenic bacteria in Solanum japonica:

[0044] Isolation and purification of pathogenic bacteria of Solanum japonica:

[0045] Isolation of bacterial strains: select leaves with typical lesion collected in natural habitat, wash them with clean water, cut out 2mm × 2mm tissue pieces at the junction of disease and health, and wash them in 70% alcohol successively on the ultra-clean workbench, Sterilize in 0.1% mercuric chloride, then rinse continuously in sterilized water for 3 times, take it out, absorb excess water with sterilized absorbent paper, put it on a PDA plate, and cultivate it in a constant temperature incubator at 25°C;

[0046] When the colony on the potato sucrose medium (PDA) plate medium grows to a certain size, it is separated and purified, and after pathogenicity determination, it is transferred to the slant of the potato sucrose medium (PDA) test tube and stored in the refrigerator at 4°C for later use;

[0...

Embodiment 2

[0070] The isolation, purification and screening of the pathogenic bacteria of Solanum japonica are based on Example 1:

[0071] Pathogenicity Test and Preparation of Crude Toxins of Pathogenic Bacteria on Solanum japonica:

[0072] Extraction of crude toxins from pathogenic bacteria:

[0073] Toxin-producing culture of Alternaria chrysalis:

[0074] On the ultra-clean workbench, transplant the Alternaria strains to the test tube potato sucrose medium (PDA) slant medium for activation, and transfer the activated Alternaria strains to the potato sucrose medium (PDA) plate medium for cultivation After 5 days, use a puncher with a diameter of 5mm to take out the fungus cake and put it into the potato dextrose (PDB) culture solution (2 pieces / 100mL), put it into a shaker for shaking culture, set the temperature at 25°C, and rotate at 110r / min , continuously cultivated for 7 days to obtain a fermentation broth;

[0075] Extraction of crude toxins:

[0076] The fermented liquid ...

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Abstract

The invention relates to application of alternaria alternata in preventing and controlling external invasion of weed solanum rostratum. Alternaria alternata is obtained by separating and purifying naturally infected plant leaves of solanum rostratum in a field natural population of Xinjiang Changji solanum rostratum, a conventional method is adopted to respectively prepare conidium suspension andcrude toxin from alternaria alternata obtained by separation and purification, the conidium suspension and the crude toxin are uniformly sprayed onto solanum rostratum plants respectively, and alternaria alternata has strong pathogenic effect and control effect on worst weed solanum rostratum. Culture operation is simple, a culture matrix material is easy to obtain, the conidium suspension and thetoxin are high in stability, the obtaining process is simple, and alternaria alternata is supportive of mass production; alternaria alternata can be used for stem-leaf spraying, is convenient in use,can effectively kill target weed solanum rostratum and has high environment safety.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to the application of a fungus Alternaria in the prevention and control of foreign invasive weeds Solanum solani. Background technique [0002] Invasion of alien organisms is a worldwide problem. Invasive organisms reduce biodiversity by replacing the original species or inhibiting the survival of new species in the invaded area, thereby destroying the stability of the ecosystem and affecting the local economic development. my country is one of the countries most seriously harmed by biological invasion. Especially in recent years, with the rapid development of economy and the increasing prosperity of international trade, some alien species have quietly entered our country through commerce, tourism and other channels. According to statistics, there are currently more than 600 species of alien invasive plants in my country, of which more than 50 species are listed among th...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P13/02
CPCA01N63/30
Inventor 邵华魏彩霞王威张弛韩彩霞
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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