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Pestalotiopsis for biocontrol of Eichhornia crassipes

A technology for the control of Polychaeta pseudodiscoides and biocontrol fungi, which is applied in the direction of fungi, microorganisms, biocides, etc., to achieve strong pathogenicity control effects, good control effects, potential commercial development and application value

Inactive Publication Date: 2013-07-03
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More than ten species of water hyacinth pathogenic fungi have been reported in my country, among which a species of pseudoalternaria (Nimbya alternantherae SF193 strain) has been protected by a Chinese patent (patent number: 200710019832), so far no A detailed report on the further research and application of several other pathogens

Method used

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  • Pestalotiopsis for biocontrol of Eichhornia crassipes
  • Pestalotiopsis for biocontrol of Eichhornia crassipes
  • Pestalotiopsis for biocontrol of Eichhornia crassipes

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1: Isolation, Purification and Screening of Herbicidal Fungi

[0026] 1. Isolation and purification of water hyacinth pathogenic bacteria

[0027]1.1. Isolation of strains: Select leaves or stems with typical lesions, wash them with clean water, cut out 2 mm × 2 mm tissue pieces at the junction of disease and health, and place them on the ultra-clean workbench at 70% Alcohol, 0.1% mercury liter disinfection, and then rinse in sterilized water for 3 consecutive times, after taking out, use sterilized absorbent paper to absorb excess water, put it on a PDA plate, and cultivate it in a constant temperature incubator at 25 ℃.

[0028] When the colony on the PDA plate medium grows to a certain size, it is separated and purified, and after being tested for pathogenicity, it is transferred to the slant of the PDA test tube and stored in the refrigerator at 4 °C for later use.

[0029] 1.2. Source and cultivation of host plants: Collect relatively healthy plants wi...

Embodiment 2

[0037] Embodiment 2: Biological characteristics of Polychaete pseudodiscoides of water hyacinth

[0038] 1. Effect of culture medium on bacterial colony growth and sporulation

[0039] On the colonies cultured at a constant temperature of 25 ℃ for 5 days, the bacteria cakes with a diameter of 5.0 mm were punched along the edge of the colonies with a puncher and inoculated on PDA, PSA, CAA, WA, WLA, OSA, WS and WD medium plates (9 cm) central. Incubate at a constant temperature of 25°C and observe continuously. After the 5th day, use the vertical cross method to measure and record the diameter of the colony daily; after the 18th day, measure the amount of sporulation, add sterilized distilled water, gently scrape the surface of the colony with a sterilized glass slide, then elute, filter, and add a certain volume of 0.1 % Tween-80 was made into a suspension, and the spore suspension was sucked up with a pipette gun and dropped on a Neubauer hemocytometer, counted under a ...

Embodiment 3

[0050] Embodiment 3: pathogenic bacteria crude toxin extraction

[0051] Toxicity-producing culture of Polychaete pseudodiscoides in water hyacinth

[0052] On the ultra-clean workbench, take out the cultivated water hyacinth plate of Polychaeta pseudodiscoides strain, use a puncher with a diameter of 5 mm to punch out the fungus cake, and put it into the potato sucrose (PS) culture solution respectively, 4 for each bottle. Truffle Pie. They were placed in a shaker for shaking culture, the temperature was set at 25 °C, the rotation speed was 110 r / min, and the darkness was alternated for 12 h, and the culture was continued for 7 days.

[0053] Crude toxin extraction

[0054] The obtained fermentation broth was filtered once with four layers of gauze, and the obtained filtrate was filtered with double-layer chromatography filter paper, and then the filtrate was centrifuged for 20 min at a speed of 10,000 r / min, and the obtained supernatant was the crude extract of the t...

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Abstract

A pestalotiopsis crassipes WH010 bacterial strain (with the storage number of CGMCCNO.4418) is obtained from water hyacinth natural disease plant leaves by means of separation, and is quite easy to produce spores under conventional culture conditions, the optimum culture medium is potato sucrose agar (PDA), the optimum temperature is 28 DEG C, the optimum pH (potential of hydrogen) is 6.5, and the best germ infection pathogenic conditions include that the inoculation concentration is larger than 105 / ml, moisture preservation is performed for 24 hours after inoculation, and the temperature is kept in a range from 25 DEG C to 28 DEG C. A bacterium preparation and toxin liquid have quite high pathopoiesis and weed killing effects for water hyacinth which is worst weed, the pestalotiopsis crassipes can be used for producing environment-friendly mycoherbicide by fermentation bacterium or toxin biomimetic synthesis technology, and has a potential business development and application value. Fungal endophyte culture supernatants are extracted by ethyl acetate and evaporated by a rotary evaporator in a vacuum manner to obtain pathogenic crude toxin. A control effect of water aqua and powder of the bacterial strain to the water hyacinth in field natural conditions can be higher than 76%. Suspension and toxin liquid of the bacterial strain are safe for 21 types of important crops such as rice, and do not have adverse effects.

Description

technical field [0001] The invention belongs to the technical field of weed biological control, and in particular relates to a water hyacinth biocontrol fungus Polychaetosporum ( Pestalotiopsis crassipes ) strains. Background technique [0002] Water hyacinth( Eichhornia crassipes ), also known as water hyacinth, is one of the top ten harmful weeds in the world and one of the important invasive plants in my country. Widely distributed in many countries and regions in Asia, North America, Oceania and Africa. Water hyacinth was introduced into my country in the 1930s and is currently widely distributed in South China, Central China and East China, especially in provinces and cities such as Yunnan, Sichuan, Hunan, Hubei, Jiangxi, Jiangsu, Zhejiang, Fujian and Taiwan. The grass has strong adaptability, rapid growth, and amazing spreading speed. It is difficult to effectively control the over-breeding, which brings serious ecological damage and biological pollution to the wat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01P13/02C12R1/645
Inventor 胡丽谭万忠
Owner SOUTHWEST UNIV
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