Small spore phoma microspora for controlling conyza sumatrensis

Abstract

The invention discloses small spore phoma microspora SMBC022 with a biological activity for controlling conyza sumatrensis, which belongs to the field of biological control over agricultural weeds. The small spore phoma microspora is separated from a host in a natural environment and is identified to be small spore phoma microspora according to morphological characteristics and molecular biology;the optimal culture medium for growing the small spore phoma microspora is a PDA (potato dextrose agar); the optimal temperature is 28 DEG C; and the optimal pH is 7.2. Pathogenic crude toxins can beobtained from a fungal culture liquid through ethyl acetate extraction. The suspension liquid of the microspora and toxin solution thereof do not have adverse effect on 13 important crops such as rice; the SMBC022 strain preparation and the toxin solution have very strong pathogenic and weed-killing effects on malignant weed conyza sumatrensis, and can be produced into an environment-friendly fungal weedicide by a fermented bacterium or toxin biomimetic synthesizing technology. The small spore phoma microspora SMBC022 has important commercial developmental value and application value.

Description

technical field [0001] The invention relates to the technical field of biological control of agricultural weeds, in particular to the use of pathogenic microorganisms with strong pathogenic and control effects on agricultural weeds as an environment-friendly biological herbicide, specifically a plant used to prevent and control Sumen liquor Phomosporum microsporum of grasses. Background technique [0002] Sumen baijiu grass is native to South America and was introduced to China in the middle of the 19th century. In recent years, Sumen baijiu grass grows rapidly, reproduces fast, produces a large number of seeds, and is widely distributed (especially along the Yangtze River). Around them, almost The growth of native plants cannot be seen, especially in places where the original vegetation has been destroyed. This kind of plants take advantage of the gap and preempt growth, forming a growth advantage, which not only causes a large reduction in the output of field crops, bu...

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Problems solved by technology

[0002] Sumen baijiu grass is native to South America and was introduced to China in the middle of the 19th century. In recent years, Sumen baijiu grass grows rapidly, reproduces fast, produces a large number of seeds, and is widely distributed (especially along the Yangtze River). Around them, almost The growth of native plants cannot be seen, especially in places where the original vegetation has been destroyed. This kind of plants take advantage of the gap and preempt growth, forming a growth advantage, which not only causes a large reduction in the output of field crops, but also affects the economic structure and society. Stable, and because it grows in large quantities in the Yangtze River Basin, it is easy to cause ecological security and cause serious consequences. It is a typical bad grass. At this stage, there are few domestic studies on Sumen liquor grass, mainly focusing on its morphological characteristics, Occurrence characteristics and hazards and other biological and ecological characteristics and control countermeasures, etc., while foreign studies on Sumen Baijiu mainly focus on the phylogenetic evolution, reproductive and breeding characteristics, photosynthetic physiological characteristics and genetics of Chrysanthemum genus

The measures to control Sumen liquorgrass in my country are mainly manual removal and the use of chemical herbicides such as glyphosate. Manual weeding is time-consuming and difficult to achieve the desired effect, and excessive use of chemical herbicides will cause environmental pollution and residual problems ( Lou Yuanlai et al., 2002)

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